Polarized Fluorescence in an Electric Field

  • G. Weill
Part of the NATO Advanced Study Institutes Series book series (NSSB, volume 64)


As for the other electro-optics effects, the change of polarization of fluorescence observed in an electric field results from the orientation of the molecules. The specific advantages which were looked for when we started to develop the technique1 were, when compared to the more classical electric birefringence and dichroism:
  • the possibility of measuring not only the second, but also the fourth moment of the orientation function and therefore to test the separation of the electrical and optical factors and the mechanism of orientation.

  • the possibility of obtaining separate information about the conformations of chromophores with essentially overlapping absorption bands but different quantum yields of fluorescence; they will appear as a single class in electric dichroism but can eventually be separated in a polarized fluorescence experiment, as for example, if one of them has zero quantum yield.


Quantum Yield Orientation Function Fourth Moment Linear Dichroism Electric Birefringence 


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Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • G. Weill
    • 1
  1. 1.Centre de Recherches sur les MacromoleculesUniversité L. PasteurStrasbourgFrance

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