The Bacterial Bioassay and Laboratory Assessments of Waste Disposal Activities at DWD-106
Changes in the bacterial uptake of 14C labeled glucose in sea-water are used to quantify some sublethal consequences of Edgemoor and Grasselli waste disposal at Deep Water Dumpsite 106.
The fractional amounts of waste in seawater which led to a 50 percent reduction in 14C uptake ranged from 0.01–0.02 percent for Edgemoor waste and from 0.10–0.20 percent for Grasselli waste.
Both Edgemoor and Grasselli wastes cause an inhibitory bacterial response which exceeds that associated with their respective acid and caustic chemical compositions. Heavy metals appear to be the principle toxic components of Edgemoor waste whereas organic species appear to produce the toxicity of Grasselli waste.
Resistance to chemical alteration as demonstrated by ultraviolet radiation and persulfate oxidation may imply an environmental persistence for Grasselli waste.
Edgemoor waste reacts with seawater with a precipitation of its heavy metal content. Such behavior is certain to influence its distribution kinetics and its impact on life processes of the ocean.
Mixtures of Edgemoor and Grasselli wastes impart an inhibitory response which is measurably less than that anticipated from the sum of their individual effects. This suggests the possibility of positive benefits from their coordinated release within the Dumpsite area.
An unambiguous approach to improve bioassay interpretations in potentially reactive, multi-waste situations was developed. The method relies upon a graphical solution to differentiate between the net response induced by mixed wastes and a hypothetical response corresponding to the sum of the individual response patterns observed independently.
KeywordsHeavy Metal Heavy Metal Content Laboratory Assessment Potassium Persulfate Woods Hole Oceanographic Institution
Unable to display preview. Download preview PDF.
- Andrews, P. and P. J. LeB. Williams (1971) Heterotrophic utilization of dissolved organic carbon in the sea. II. Measurements of the oxidation rates and concentrations of glucose and amino acids in seawater. J. Mar. Biol. Assoc. U.K., 51, 121–126.Google Scholar
- Parsons, T. R. and H. Seki (1970) Importance and general implications of organic matter in aquatic environments. In: Organic Matter in Natural Waters, D. W. Hood, editor, Univ. Alaska, 1-27.Google Scholar
- Sunda, W. G. and R. R. L. Guillard (1976) The relationship between cupric ion activity and the toxicity of copper to phytoplankton. J. Mar. Res., 34, 511–529.Google Scholar
- Vaccaro, R. F., F. Azam and R. E. Hodson (1977) Response of natural marine bacterial populations to copper: Controlled ecosystem pollution experiment. Bull. Mar. Sci., 27, 17–22.Google Scholar