The Bacterial Bioassay and Laboratory Assessments of Waste Disposal Activities at DWD-106

  • Ralph F. Vaccaro
  • Mark R. Dennett
Part of the Marine Science book series (MR, volume 12)


Changes in the bacterial uptake of 14C labeled glucose in sea-water are used to quantify some sublethal consequences of Edgemoor and Grasselli waste disposal at Deep Water Dumpsite 106.

The fractional amounts of waste in seawater which led to a 50 percent reduction in 14C uptake ranged from 0.01–0.02 percent for Edgemoor waste and from 0.10–0.20 percent for Grasselli waste.

Both Edgemoor and Grasselli wastes cause an inhibitory bacterial response which exceeds that associated with their respective acid and caustic chemical compositions. Heavy metals appear to be the principle toxic components of Edgemoor waste whereas organic species appear to produce the toxicity of Grasselli waste.

Resistance to chemical alteration as demonstrated by ultraviolet radiation and persulfate oxidation may imply an environmental persistence for Grasselli waste.

Edgemoor waste reacts with seawater with a precipitation of its heavy metal content. Such behavior is certain to influence its distribution kinetics and its impact on life processes of the ocean.

Mixtures of Edgemoor and Grasselli wastes impart an inhibitory response which is measurably less than that anticipated from the sum of their individual effects. This suggests the possibility of positive benefits from their coordinated release within the Dumpsite area.

An unambiguous approach to improve bioassay interpretations in potentially reactive, multi-waste situations was developed. The method relies upon a graphical solution to differentiate between the net response induced by mixed wastes and a hypothetical response corresponding to the sum of the individual response patterns observed independently.


Heavy Metal Heavy Metal Content Laboratory Assessment Potassium Persulfate Woods Hole Oceanographic Institution 
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  1. Aaronson, S. (1978) Excretion of organic matter by phytoplankton in vitro, Limnol. Oceanogr., 23, 838.CrossRefGoogle Scholar
  2. Andrews, P. and P. J. LeB. Williams (1971) Heterotrophic utilization of dissolved organic carbon in the sea. II. Measurements of the oxidation rates and concentrations of glucose and amino acids in seawater. J. Mar. Biol. Assoc. U.K., 51, 121–126.Google Scholar
  3. Armstrong, F. A. J., P. M. Williams and J. D. H. Strickland (1966) Photooxidation of organic matter in seawater by ultraviolet radiation, analytical and other applications. Nature, 211, 481–483.CrossRefGoogle Scholar
  4. Gillespie, P. A. and R. F. Vaccaro (1978) A bacterial bioassay for measuring the copper-chelation capacity of seawater. Limnol. Oceanogr., 23, 543–548.CrossRefGoogle Scholar
  5. Hobbie, J. E. and R. T. Wright (1965) Bioassay with bacterial uptake kinetics: glucose in freshwater. Limnol. Oceanogr., 10, 471–474.CrossRefGoogle Scholar
  6. Lampert, W. (1978) Release of dissolved organic carbon by grazing Zooplankton. Limnol. Oceanogr., 23, 831–834.CrossRefGoogle Scholar
  7. Menzel, D. W. and R. F. Vaccaro (1964) The measurement of dissolved organic and particulate carbon in seawater. Limnol. Oceanogr. 2, 138–142.CrossRefGoogle Scholar
  8. Parsons, T. R. and J. D. H. Strickland (1961) On the production of particulate organic oceanic carbon by heterotrophic processes in seawater. Deep-Sea Res., 8, 211–222.CrossRefGoogle Scholar
  9. Parsons, T. R. and H. Seki (1970) Importance and general implications of organic matter in aquatic environments. In: Organic Matter in Natural Waters, D. W. Hood, editor, Univ. Alaska, 1-27.Google Scholar
  10. Sunda, W. G. and R. R. L. Guillard (1976) The relationship between cupric ion activity and the toxicity of copper to phytoplankton. J. Mar. Res., 34, 511–529.Google Scholar
  11. Sunda, W. G. and J. A. M. Lewis (1978) Effect of complexation by natural organic ligands on the toxicity of copper to a unicellular algal Monochrysis lutheri. Limnol. Oceanogr., 23, 870–876.CrossRefGoogle Scholar
  12. Vaccaro, R. F. (1966) Studies on heterotrophic activity in seawater based on glucose assimilation. Limnol. Oceanogr., 11, 596–607.CrossRefGoogle Scholar
  13. Vaccaro, R. F., F. Azam and R. E. Hodson (1977) Response of natural marine bacterial populations to copper: Controlled ecosystem pollution experiment. Bull. Mar. Sci., 27, 17–22.Google Scholar
  14. Webb, K. L. and R. E. Johannes (1967) Studies of the release of dissolved free amino acids by marine Zooplankton, Limnol. Oceanogr., 12, 376–382.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Ralph F. Vaccaro
    • 1
  • Mark R. Dennett
    • 1
  1. 1.Woods Hole Oceanographic InstitutionWoods HoleUSA

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