Assay Methods for Prostaglandins and Thromboxanes: Gas Chromatographic — Mass Spectrometric Methods and Radioimmunoassay

  • Elisabeth Granström
Part of the NATO Advanced Study Institutes Series book series


The metabolites of polyunsaturated fatty acids constitute a large and steadily growing family of compounds, with the classical prostaglandins as the first identified members, and the thromboxanes, prostacyclins and leukotrienes as more recently discovered products. Most kinds of studies in this increasingly complicated area involve detection and often also quantitation of these compounds, which generally occur in extremely small amounts. Thus, sensitive and specific assay methods are needed.


Stannous Chloride Deuterium Atom Prostaglandin Endoperoxide Enoic Acid Narrow Mass Range 


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  1. 1.
    E. Granström and B. Samuelsson, Quantitative measurement of prostaglandins and thromboxanes: General considerations. In: “Advances in Prostaglandin and Thromboxane Research,” Vol. 5, J. C. Frölich ed., Raven Press, New York, (1978), pp. 1–13.Google Scholar
  2. 2.
    B. Samuelsson, M. Ramberg and C. Sweeley, Quantitative gas chromatography of prostaglandin El at the nanogram level: Use of deuterated carrier and multiple-ion analyzer. Anal. Biochem., 38: 301 (1970).PubMedCrossRefGoogle Scholar
  3. 3.
    K. Green, M. Ramberg, B. Samuelsson, M. Smigel and J. C.Frölich, Measurement of prostaglandins, thromboxanes, prostacyclin and their metabolites by gas-liquid chromatography–mass spectrometry. In: “Advances in Prostaglandin and Thromboxane Research”, Vol. 5. J. C. Frölich ed., Raven Press, New York, (1978), pp. 39–94.Google Scholar
  4. 4.
    K. Green, E. Granström, B. Samuelsson and U. Axen, Methods for quantitative analysis of PGF2a, PGE2, 9a, lla-dihydroxy15-keto-prost-5-enoic acid and 9a,11a,15-trihydroxyprost-5enoic acid from body fluids using deuterated carriers and gas chromatography - mass spectrometry. Anal. Biochem. 54: 434 (1973).PubMedCrossRefGoogle Scholar
  5. 5.
    B. Samuelsson, M. Goldyne, E. Granström, M. Ramberg, S. Hammarström and C. Malmsten, Prostaglandins and thromboxanes. Ann. Rev. Biochem., 47: 997 (1978).PubMedCrossRefGoogle Scholar
  6. 6.
    E. Granström, Radioimmunoassay of prostaglandins. Prostaglandins, 15: 3 (1978).PubMedGoogle Scholar
  7. 7.
    E. Granström and H. Kindahl, Radioimmunoassay of prostaglandins and thromboxanes. In: “Advances in Prostaglandin and Thromboxane Research”, Vol. 5. J. C. Frölich ed., Raven Press, New York (1978), pp. 119–210.Google Scholar
  8. 8.
    M. Hambers, J. Svensson, T. Wakabayashi and B. Samuelsson, Isolation and structure of two prostaglandin endoperoxides that cause platelt aggregation. Proc. Natl. Acad. Sci. USA, 71: 345 (1974).CrossRefGoogle Scholar
  9. 9.
    M. Hamberg, J. Svenssen and B. Samuelsson, Prostaglandin endoperoxides. A new concept concerning the mode of action and release of prostaglandins. Proc. Natl. Acad. Sci. USA, 71: 3824, (1974).CrossRefGoogle Scholar
  10. 10.
    M. Hamberg, J. Svensson and B. Samuelsson, Thromboxanes: A new group of biologically active compounds derived from prostaglandin endoperoxides. Proc. Natl. Acad. Sci. USA, 72: 2994 (1975).PubMedCrossRefGoogle Scholar
  11. 11.
    E. Granström, H. Kindahl and B. Samuelsson, A method for measuring the unstable thromboxane A2: Radioimmunoassay of the derived mono-0-methyl-thromboxane B2. Prostaglandins, 12: 929 (1976).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Elisabeth Granström
    • 1
  1. 1.Department of ChemistryKarolinska InstitutetStockholmSweden

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