Serotonin Binding Protein: Role in Transmitter Storage in Central and Peripheral Serotonergic Neurons
Serotonin binding protein was first found in the brain in 1974 (Tamir and Huang, 1974). The regional distribution of SBP in the brain (Tamir and Kuhar, 1975) and spinal cord (Tamir and Gershon, 1979) was shown to parallel that of serotonin (5-HT). Since, in addition, the amount of SBP in the forebrain is decreased by lesioning the nuceli of the median raphe it seems likely that the protein is an intrinsic component of serotonergic neurons (Tamir and Kuhar, 1975). This conclusion is supported by the observation that SBP accumulates above, and decreases below, lesions of the spinal cord (Tamir and Gershon, 1979). Although SBP has only partially been purified, the unusually high affinity of the protein for 5-HT, the inhibition of 5-HT bindin by reserpine, and the characteristic dependence of SBP on Fe+2 for 5-HT binding enable SBP to be recognized (Tamir et al., 1976). The 5-HT-Fe+2-SBP complex has a mobility on 10% SDS-polyacrylamide gels corresponding to 60,000 daltons (figure 1).
KeywordsSynaptic Vesicle Myenteric Plexus Serotonergic Neuron Spinal Cord Lesion Median Raphe
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