Serotonin pp 327-345 | Cite as

Serotonin-Receptors Coupled with an Adenylate Cyclase in the Rat Brain: Non-Identity with 3H-5-HT Binding Sites

  • J. Bockaert
  • D. L. Nelson
  • A. Herbet
  • J. Adrien
  • A. Enjalbert
  • M. Hamon
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 133)


Recently, two biochemical approaches have been used for studying serotonin (5-HT) receptors in the central nervous system (CNS). One consists of measuring the stimulation of a specific adenylate cyclase by 5-HT (Ahn and Makman,1978; Daszuta et al., 1979; Enjalbert et al., 1978 a,b; Fillion et al.,1979; Nelson et al.,1979 a,b; Pagel et al.,1976; Von Hungen et aí.,1975) while the other involves the labelling of specific receptors with 3H-5-HT (Bennett and Snyder,1976; Fillion et al.,1976; 1978; Nelson et al.1978; 1979 a,b; Schwarcz et aí.,1977), 3H-LSD (Bennett and Snyder,1976; Fillion et al.1978) or 3H-spiroperidol (Creese and Snyder,1978; Hamon et al.,1979 b; Leysen et al., (1978). An important question is whether the specific 5-HT receptors detected with these different biochemical tools are identical or not. So far, this question has not been solved. In this report, the kinetic characteristics, ontogenetic development, topographical and subcellular distributions and the pharmacological properties of the 5-HT-sensitive adenylate cyclase and 3H-5-HT binding sites have been compared.


Adenylate Cyclase Kainic Acid Adenylate Cyclase Activity Lumbar Spinal Cord Ontogenetic Development 
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Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • J. Bockaert
    • 1
  • D. L. Nelson
    • 2
  • A. Herbet
    • 2
  • J. Adrien
    • 3
  • A. Enjalbert
    • 1
  • M. Hamon
    • 2
  1. 1.Laboratoire de Physiologie cellulaireCollège de FranceParis Cedex 05France
  2. 2.Groupe NB-INSERM U-114Collège de FranceParis Cedex 05France
  3. 3.Unité de Recherches neurophysiologiquesINSERM U-3ParisFrance

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