Serotonin pp 327-345 | Cite as

Serotonin-Receptors Coupled with an Adenylate Cyclase in the Rat Brain: Non-Identity with 3H-5-HT Binding Sites

  • J. Bockaert
  • D. L. Nelson
  • A. Herbet
  • J. Adrien
  • A. Enjalbert
  • M. Hamon
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 133)

Abstract

Recently, two biochemical approaches have been used for studying serotonin (5-HT) receptors in the central nervous system (CNS). One consists of measuring the stimulation of a specific adenylate cyclase by 5-HT (Ahn and Makman,1978; Daszuta et al., 1979; Enjalbert et al., 1978 a,b; Fillion et al.,1979; Nelson et al.,1979 a,b; Pagel et al.,1976; Von Hungen et aí.,1975) while the other involves the labelling of specific receptors with 3H-5-HT (Bennett and Snyder,1976; Fillion et al.,1976; 1978; Nelson et al.1978; 1979 a,b; Schwarcz et aí.,1977), 3H-LSD (Bennett and Snyder,1976; Fillion et al.1978) or 3H-spiroperidol (Creese and Snyder,1978; Hamon et al.,1979 b; Leysen et al., (1978). An important question is whether the specific 5-HT receptors detected with these different biochemical tools are identical or not. So far, this question has not been solved. In this report, the kinetic characteristics, ontogenetic development, topographical and subcellular distributions and the pharmacological properties of the 5-HT-sensitive adenylate cyclase and 3H-5-HT binding sites have been compared.

Keywords

Serotonin Clozapine Piperazine Creatine Phosphate Tryptamine 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Adrien, J., 1975, Développement du sommeil lent après lésion des noyaux raphé antérieurs chez le rat nouveau-né, C.R.Acad. Sci.(Paris), 280: 1725.Google Scholar
  2. Ahn, H.S. and Makman, M.H., 1978, Stimulation of adenylate cyclase activity in monkey anterior limbic cortex by serotonin, Brain Res., 153: 636.CrossRefGoogle Scholar
  3. Beaudet, A. and Descarries, L., 1978, The monoamine innervation of rat cerebral cortex: synaptic and non-synaptic axon terminals, Neuroscience, 3: 851.PubMedCrossRefGoogle Scholar
  4. Bennett, J.P. Jr. and Snyder, S.H.,1976, Serotonin and lysergic acid diethylamide binding in rat brain membranes : relationship to postsynaptic serotonin receptors, Mol. Pharmacol., 12 : 373.PubMedGoogle Scholar
  5. Bourgoin, S., Enjalbert, A., Adrien, J., Héry, F. and Hamon, M., 1977, Midbrain raphe lesion in the new-born ra: II bio-chemical alterations in serotoninergic innvervation, Brain Res., 127: 111.PubMedCrossRefGoogle Scholar
  6. Campochiaro, P. and Coyle, J.T., 1978, Ontogenetic development of kainate neurotoxicity: correlates with glutamatergic innervation, Proc. Natl. Acad. Sci.USA, 75: 2025.PubMedCrossRefGoogle Scholar
  7. Coyle, J.T. and Schwarcz, R., 1976, Lesions of striatal neurons with kainic acid provides a model for Huntington’s chorea, Nature, 263: 244.PubMedCrossRefGoogle Scholar
  8. Creese, I. and Snyder, S.H., 1978, 3H-spiroperidol labels serotonin receptors in rat cerebral cortex and hippocampus, Eur. J. Pharmacol., 49 : 201.PubMedCrossRefGoogle Scholar
  9. Daszuta, A., Pons, F. and Cadilhac, J., 1979, Effect of serotonin on cyclic AMP level in rat hypothalamus slices during development, Eur. J. Pharmacol., 56: 397.PubMedCrossRefGoogle Scholar
  10. Dolphin, A. Adrien, J., Hamon, M. and Bockaert, J., 1979, Identity of iH-dihydroalprenolol binding sites and ß-adrenergic receptors coupled with adenylate cyclase in the central nervous system: Pharmacological properties, distribution and adaptive responsiveness, Mol. Pharmacol., 15: 1.PubMedGoogle Scholar
  11. Enjalbert, A., Bourgoin, S., Hamon, M., Adrien, J. and Bockaert, J., 1978 a, Postsynaptic serotonin-sensitive adenylate cyclase in the central nervous system. I. Development and distribution of serotonin and dopamine-sensitive adenylate cyclases in rat and guinea pig brain, Mol. Pharmacol.,14 :2.PubMedGoogle Scholar
  12. Enjalbert, A., Hamon, M., Bourgoin, S. and Bockaert, J., 1978 b, Postsynaptic serotonin-sensitive adenylate cyclase in the central nervous system. II. Comparison with dopamine-and isoproterenol-sensitive adenylate cyclases in rat brain, Mol. Pharmacol., 14 : 11.PubMedGoogle Scholar
  13. Fillion, G., Fillion, M.P., Spirakis, C., Balhers, J.M. and Jacob, J., 1976, 5-hydroxytryptamine binding to synaptic membranes from rat brain, Life Sci., 18 : 65.PubMedCrossRefGoogle Scholar
  14. Fillion, G., Rousselle, J.C., Beaudoin, D., Pradelles, P., Goiny,M., Dray, F. and Jacob, J., 1979, Serotonin-sensitive adenylate cyclase in horse brain synaptosomal membranes, Life Sci., 24: 1813.PubMedCrossRefGoogle Scholar
  15. Fillion, G.M.B., Rousselle, J.C., Fillion, M.P., Beaudoin, D.M., Goiny, M.R., Deniau, J.M. and Jacob, J.J., 1978, High-affinity binding of 3H-5-hydroxytryptamine brain synaptosomal membranes: comparison with 3H-lysergic acid diethylamide binding, Mol. Pharmacol., 14: 50.PubMedGoogle Scholar
  16. Gaddum, J.H. and Picarelli, Z.P., 1957, Two types of tryptamine receptors, Brit. J. Pharmacol., 12: 323.Google Scholar
  17. Gerschenfeld, H.M. and Paupardin-Tritsch, D., 1974, Ionic mechanisms and receptor properties underlying the responses of moluscan neurons to 5-hydroxytryptamine, J. Physiol.,(Lond.)243: 427.Google Scholar
  18. Hamon, M., Bourgoin, S., Enjalbert, A., Bockaert, J., Héry, F., Ternaux, J.P. and Glowinski, J., 1976, The effects of quipazine on 5-HT metabolism in the rat brain, Naunyn. Schmiedeberg’s Arch. Pharmacol., 294: 99.Google Scholar
  19. Hauron, M., Nelson, D.L., Herbet, A., Bockaert, J. and Glowinski,J., 1979, Characteristics of serotonin receptors in the rat brain in “Neuroactive compounds and their cell receptors” 24th Oholo Conference, Karger A.G., Basel (in press).Google Scholar
  20. Hamon, M., Nelson, D.L., Herbet, A. and Glowinski, J., 1979, Multiple receptors for serotonin in the rat brain, in “Receptors neurotransmitters and peptide hormones” Eds. S.J. Enna, M.J. Kuhar and G.C. Pepeu, Raven Press, N.Y. (in press).Google Scholar
  21. Laduron, P.M., Verwimp, M.F., Janssen, P.F.M. and Gommereh, W.R., 1975, Tissue fractionation in rat brain, kidney and liver. I. Intracellular localization of 5-methyltetrahydrofolic requiring enzyme, Biochimie, 57: 253.PubMedCrossRefGoogle Scholar
  22. Levitzki, A., 1978, The mode of coupling of adenylate cyclase to hormone receptors and its modulation by GTP, Biochem. Pharmacol., 27: 2083.PubMedCrossRefGoogle Scholar
  23. Leysen, J. and Laduron, P., 1977, Differential distribution of opiate and neuroleptic receptors and dopamine-sensitive adenylate cyclase in rat brain, Life. Sci., 20: 281.PubMedCrossRefGoogle Scholar
  24. Leysen, J.E., Niemegeers, C.J.E., Tollenaere, J.P. and Laduron,P.M., 1978, Serotonergic component of neuroleptic receptors, Nature, 272: 168.Google Scholar
  25. Mac Dermot, J., Higashida, H., Wilson, S.P., Matsuzawa, H., Minna,J. and Nirenberg, M., 1979, Adenylate cyclase and acetylcholine release regulated by separate serotonin receptors of somatic hybrid cells, Proc. Natl. Acad. Sci.USA, 76: 1135.CrossRefGoogle Scholar
  26. Nelson, D.L., Herbet, A., Adrien, J., Bockaert, J. and Hamon, M., 1979, Serotonin-sensitive adenylate cyclase and 3H-serotonin binding sites. I I. Respective subcellular and regional distributions and ontogenetic developments in the CNS of the rat, Mol. Pharmacol. (submitted).Google Scholar
  27. Nelson, D.L., Herbet, A., Bourgain, S., Glowinski, J. and Hamon,M., 1978, Characteristics of central 5-HT receptors and their adaptive changes following intracerebral 5,7-dihydroxytryptamine administration in the rat, Mol. Pharmacol., 14: 983.PubMedGoogle Scholar
  28. Nelson, D.L., Herbet, A., Enjalbert, A., Bockaert, J. and Hamon,M., 1979, Serotonin-sensitive adenylate cyclase and 3H-serotonin binding sites. I. Respective kinetic and pharmacologic properties in the CNS of the rat, Mol. Pharmacol., (submitted).Google Scholar
  29. Pagel, J., Christian, S.T., Quayle, E.S. and Monti, J.A., 1976, A serotonin-sensitive adenylate cyclase in mature rat brain synaptic membranes, Life Sci., 19: 819.PubMedCrossRefGoogle Scholar
  30. Salomon, Y., Londos, C. and Rodbell, M., 1974, A highly sensitive adenylate cyclase assay, Analyt. Biochem., 58: 541.PubMedCrossRefGoogle Scholar
  31. Schwarcz, R., Bennett, J.P. Jr. and Coyle, J.T. Jr., 1977, Loss of striatal serotonin synaptic receptor binding induced by kainic acid lesions: correlations with Huntington’s disease, J. Neurochem., 28: 867.PubMedCrossRefGoogle Scholar
  32. Schwarcz, R. and Coyle, J.T., 1977, Striatal lesions with kainic acid: neurochemical characteristics, Brain Res., 127: 235.PubMedCrossRefGoogle Scholar
  33. Von Hungen, K., Roberts, S. and Hill, D.F., 1975, Serotoninsensitive adenylate cyclase activity in immature rat brain, Brain Res., 84: 257.CrossRefGoogle Scholar
  34. Zecevic, N.R. and Molliver, M.E., 1978, The origin of the monoaminergic innervation of immature rat neocortex: on ultra-structural analysis following lesions, Brain Res., 150: 387.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • J. Bockaert
    • 1
  • D. L. Nelson
    • 2
  • A. Herbet
    • 2
  • J. Adrien
    • 3
  • A. Enjalbert
    • 1
  • M. Hamon
    • 2
  1. 1.Laboratoire de Physiologie cellulaireCollège de FranceParis Cedex 05France
  2. 2.Groupe NB-INSERM U-114Collège de FranceParis Cedex 05France
  3. 3.Unité de Recherches neurophysiologiquesINSERM U-3ParisFrance

Personalised recommendations