Advertisement

Mutation-Induction in Repair-Deficient Strains of Neurospora

  • Frederick J. de Serres
Part of the Basic Life Sciences book series (BLSC, volume 15)

Summary

Various mutants sensitive to UV-induced inactivation have been used to study the process of spontaneous and induced mutation in the ad-3 region of Neurospora crassa. Studies on haploid strains have shown that the process of mutation-induction in the ad-3 region is under genetic control. Studies on two-component heterokaryons have shown that this control effects both point mutations and multi-locus deletions. Comparisons made between an excision-repair deficient two-component heterokaryon (59) have shown that the level of effect is markedly mutagen-specific. All possible effects on the process of mutation-induction in the ad-3 region have been found in the strains tested.

Keywords

Neurospora Crassa Xeroderma Pigmentosum Haploid Strain Methyl Methanesulfonate Xeroderma Pigmentosum Patient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    de Serres, F. J., Carbon dioxide stimulation of the ad-3 mutants Neurospora crassa, Mutat. Res., 3 (1966) 420–425.PubMedCrossRefGoogle Scholar
  2. 2.
    de Serres, F. J., Mutability of the UV-sensitive strains of Neurospora crassa, Genetics, 68 (1971) 14–15.Google Scholar
  3. 3.
    de Serres, F. J., Mutagenic specificity of chemical carcinogens in microorganisms. In: Proceedings of Workshop on Approaches to Assess the Significance of Experimental Chemical Carcinogenesis Data for Man, December 10–12, 1973, Brussels, Belgium (IARC Scientific Publication 10), IARC, Lyon, 1974, pp. 201–211.Google Scholar
  4. 4.
    de Serres, F. J., The utilization of leaky ad-3 mutants of Neurospora crassa in heterokaryon tests for allelic complementation, Mutat. Res., 3 (1966) 3–12.PubMedCrossRefGoogle Scholar
  5. 5.
    de Serres, F. J., Qualitative effects of UV-sensitive strains of Neurospora crassa on mutation induction at the ad-3B locus, Mutat. Res., 21 (1973) 216–217.Google Scholar
  6. 6.
    de Serres, F. J., Unpublished data.Google Scholar
  7. 7.
    de Serres, F. J., H. E. Brockman, C. Y. Hung, and T. M. Ong, Comparison of the mutagenic effects of chemical mutagens in excision-repair deficient and wild-type two-component heterokaryons of Neurospora crassa. Presented at the 9th Annual Meeting, Environmental Mutagen Society, San Francisco, California, March 9–13, 1978, Program and Abstracts Book, pp. 51–52.Google Scholar
  8. 8.
    de Serres, F. J., H. E. Brockman, C. Y. Hung, and T. M. Ong, Comparison of the mutagenic effects of 2-aminopurine and actinomycin D in excision-repair deficient and wild-type two-component heterokaryons of Neurospora crassa, Environ. Mut., 1 (1979) 132.Google Scholar
  9. 9.
    de Serres, F. J., H. E. Brockman, W. E. Barnett, and H. G. Kolmark, Allelic complementation among nitrous acid-induced ad-3B mutants of Neurospora crassa, Mutat. Res., 4 (1967) 415–424.PubMedCrossRefGoogle Scholar
  10. 10.
    de Serres, F. J., and H. V. Malling, Measurement of recessive lethal damage over the entire genome and at two specific loci in the ad-3 region of a two-component heterokaryon of Neurospora crassa, In: Chemical Mutagens: Principles and Methods for their Detection, Vol. II, A. Hollaender, Ed., Plenum Press, New York, 1971, pp. 311–342.Google Scholar
  11. 11.
    de Serres, F. J., and M. E. Schüpbach, Mutagenesis at the ad-3A and ad-3B loci in haploid UV-sensitive strains of Neurospora crassa. II. Comparison of γ-ray induced inactivation and mutation-induction dose-response curves, Mutat. Res., in press.Google Scholar
  12. 12.
    Inoue, H., T. M. Ong, and F. J. de Serres, Inactivation and mutation induction by ICR-170 in UV-sensitive strains of Neurospora crassa, Mutat. Res., 38 (1976) 379.Google Scholar
  13. 13.
    Inoue, H., T. M. Ong, and F. J. de Serres, Lethal and mutagenic effects of 4-nitroquinoline-1-oxide in UV-sensitive strains of Neurospora crassa, Mutat. Res., 53 (1978) 82–83.Google Scholar
  14. 14.
    Inoue, H., T. M. Ong, and F. J. de Serres, Lethal and mutagenic effects of N-methyl-N′-nitro-N-nitrosoguanidine in UV-sensitive strain of Neurospora crassa, Mutat. Res., 31 (1975) 307–308.Google Scholar
  15. 15.
    Kilbey, B. J., F. J. de Serres, and H. V. Malling, Identification of the genetic alteration at the molecular level of ultraviolet light-induced ad-3B mutants in Neurospora crassa, Mutat. Res., 12 (1971) 47–56.PubMedCrossRefGoogle Scholar
  16. 16.
    Maher, V. M., J. J. McCormick, P. L. Grover, and P. Sims, Effect of DNA repair on the cytotoxicity and mutagenicity of polycyclic hydrocarbons derivatives in normal and xeroderma pigmentosum human fibroblasts, Mutat. Res., 43 (1977) 117–138.PubMedCrossRefGoogle Scholar
  17. 17.
    Maher, V. M., L. M. Ouellette, R. D. Curren, and J. J. McCormick, Frequency of ultraviolet light-induced mutations is higher in xeroderma pigmentosum variant cells than in normal cells, Nature 261 (1976) 593–595.PubMedCrossRefGoogle Scholar
  18. 18.
    Malling, H. V., and F. J. de Serres, Correlation between basepair transition and complementation pattern in nitrous acid-induced ad-3B mutants of Neurospora crassa, Mutat. Res., 5 (1968) 359–371.PubMedCrossRefGoogle Scholar
  19. 19.
    Malling, H. V., and F. J. de Serres, Identification of genetic alterations induced by ethylmethanesulfonate in Neurospora crassa, Mutat. Res., 6 (1968) 181–193.PubMedCrossRefGoogle Scholar
  20. 20.
    Malling, H. V., and F. J. de Serres, Identification of the spectrum of x-ray-induced intragenic alterations at the molecular level in Neurospora crassa, Rad. Res., 31 (1967) 637–638.Google Scholar
  21. 21.
    Malling, H. V., and F. J. de Serres, Relation between complementation patterns and genetic alterations in nitrous acid-induced ad-3B mutants of Neurospora crassa, Mutat. Res., 4 (1967) 425–440.PubMedCrossRefGoogle Scholar
  22. 22.
    Russell, L. B., Definition of functional units in a small chromosomal segment of the mouse and its use in interpreting the nature of radiation-induced mutation, Mutat. Res., 11 (1971) 107–123.PubMedCrossRefGoogle Scholar
  23. 23.
    Sasaki, M. S., DNA repair capacity and susceptibility to chromosome breakage in xeroderma pigmentosum cells, Mutat. Res., 20 (1973) 291–293.PubMedCrossRefGoogle Scholar
  24. 24.
    Schroeder, A. L., Genetic control of radiation sensitivity and DNA repair in Neurospora, In: Molecular Mechanisms for Repair of DNA, Part B, P. C. Hanawalt and R. B. Setlow, Eds., Plenum Press, New York, 1975, pp. 567–576.Google Scholar
  25. 25.
    Worthy, T. E., and J. L. Epler, Biochemical basis of radiation-sensitivity in mutants of Neurospora crassa, Mutat. Res., 19 (1973) 167–173.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Frederick J. de Serres
    • 1
  1. 1.National Institute of Environmental Health SciencesResearch Triangle ParkUSA

Personalised recommendations