Mutagen-Sensitive Mutants in Neurospora

  • Alice L. Schroeder
  • Loree D. Olson
Part of the Basic Life Sciences book series (BLSC, volume 15)


Initial work on the fungus Neurospora crassa has shown that at least two DNA-repair systems exist in this eukaryote: excision repair and a mutation-prone repair. The evidence suggests that there is also a third repair system. Recently, new mutagen-sensitive strains have been isolated in several laboratories, but they are not yet fully characterized. A hunt for cytoplasmically inherited UV sensitivity has failed to turn up any such mutants among 25 new UV-sensitive isolates.


Neurospora Crassa Sensitive Mutant Cytoplasmic Factor Thymine Dimer Mendelian Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Boyce, R. P., and P. Howard-Flanders, Release of ultraviolet light-induced thymine dimers from DNA in E. coli, Proc. Natl. Acad. Sci. (U.S.), 51 (1964) 293–300.CrossRefGoogle Scholar
  2. 2.
    Chang, L. T., and R. W. Tuveson, Ultraviolet-sensitive mutants in Neurospora crassa, Genetics, 56 (1967) 801–810.PubMedGoogle Scholar
  3. 3.
    Chang, L. T., R. W. Tuveson, and M. H. Munroe, Non-nuclear inhertiance of UV sensitivity in Neurospora crassa, Can. J. Genet. Cytol., 10 (1968) 920–927.PubMedGoogle Scholar
  4. 4.
    Clark, A. J., and A. D. Margulies, Isolation and characterization of recombination-deficient mutants of Escherichia coli K-12, Proc. Natl. Acad. Sci. (U.S.), 53 (1965) 451–459.CrossRefGoogle Scholar
  5. 5.
    Fincham, J. R. S., and P. R. Day, in: Fungal Genetics, 3rd ed., Botanical Monographs, Vol. 4, Blackwell Scientific, Oxford-Edinburgh, 1971, pp. 314, 320.Google Scholar
  6. 6.
    Fraser, M. J., S. Kwong, D. M. Galer, and T. Y.-K. Chow, Regulation by proteinases of a putative Rec nuclease of Neurospora, in: DNA Repair Mechanisms, P. C. Hanawalt, E. C. Friedberg, and C. F. Fox, Eds., Academic Press, New York, 1978, pp. 441–444.Google Scholar
  7. 7.
    Fraser, M. G., Alkaline deoxyribonucleases released from Neurospora crassa mycelia: two activities not released by mutants with multiple sensitivities to mutagens, Nucl. Acids Res., 6 (1979) 231–246.PubMedCrossRefGoogle Scholar
  8. 8.
    Fraser, M. J., T. Y.-K. Chow, and E. Käfer, Nucleases and their control in wild-type and nuh mutants of Neurospora, This volume, p. 63.Google Scholar
  9. 9.
    Howard-Flanders, P., and R. P. Boyce, DNA repair and genetic recombination: studies on mutants of Escherichia coli defective in these processes, Radiat. Res. (Suppl.), 6 (1966) 156–181.CrossRefGoogle Scholar
  10. 10.
    Ishikawa, T., A. Toh-e, I. Unc, and K. Hasunuma, Isolation and characterization of nuclease mutants of Neurospora crassa, Genetics, 63 (1969) 75–95.PubMedGoogle Scholar
  11. 11.
    Joester, W., K.-E. Joester, B. van Dorp, and P. H. Hofschneider, Purification and properties of DNA-dependent DNA-polymerases from Neurospora crassa, Nucl. Acids Res., 5 (1978) 3043–3055.PubMedCrossRefGoogle Scholar
  12. 12.
    Käfer, E., Sensitivity to methyl-methane-sulfonate in Neurospora, Neurospora Newsl., 25 (1978) 19.Google Scholar
  13. 13.
    Käfer, E., and M. Fraser, Isolation and genetic analysis of nuclease halo (nuh) mutants of Neurospora crassa, Mol. Gen. Genet., 169 (1979) 117–127.PubMedCrossRefGoogle Scholar
  14. 14.
    Mehta, R. D., and J. Weijer, U.V. mutability in gamma-ray-sensitive mutants of Neurospora crassa, in: Symposium on Use of Radiation and Radioisotopes for Genetic Improvement of Industrial Microorganisms, International Atomic Energy Agency, Vienna, 1971, pp. 63–71.Google Scholar
  15. 15.
    Newmeyer, D., and D. R. Galeazzi, The instability of Neurospora duplication Dp(I1→IR) H4250 and its genetic control, Genetics, 85 (1977) 461–487.PubMedGoogle Scholar
  16. 16.
    Newmeyer, D., A. L. Schroeder, and D. R. Galeazzi, An apparent connection between histidine recombination and repair in Neurospora, Genetics, 89 (1978) 271–279.PubMedGoogle Scholar
  17. 17.
    Prakash, L., The relation between repair of DNA and radiation and chemical mutagenesis in Saccharomyces cerevisiae, Mutat. Res., 41 (1976) 241–248.PubMedCrossRefGoogle Scholar
  18. 18.
    Raju, N. B., and D. D. Perkins, Barren perithecia in Neurospora crassa, Can. J. Genet. Cytol. 20 (1978) 54–59.Google Scholar
  19. 19.
    Schroeder, A. L., Ultraviolet-sensitive mutants of Neurospora, I. Genetic basis and effect on recombination, Mol. Gen. Genet., 107 (1970) 291–304.PubMedCrossRefGoogle Scholar
  20. 20.
    Schroeder, A. L., Ultraviolet-sensitive mutants of Neurospora, II. Radiation studies, Mol. Gen. Genet., 107 (1970) 305–320.PubMedCrossRefGoogle Scholar
  21. 21.
    Schroeder, A. L., Genetic control of radiation sensitivity and DNA repair in Neurospora, in: Molecular Mechanisms for Repair of DNA, P. C. Hanawalt and R. B. Setlow, Eds., Part B, Plenum Press, New York, 1975, pp. 567–576.Google Scholar
  22. 22.
    Schroeder, A. L., and S. L. Norton, unpublished data.Google Scholar
  23. 23.
    Setlow, R. B., and W. L. Carrier, The disappearance of thymine dimers from DNA: an error correcting mechanism, Proc. Natl. Acad. Sci. (U.S.), 51 (1964) 226–231.CrossRefGoogle Scholar
  24. 24.
    Shelby, M. D., F. J. de Serres, and Gerald J. Stine, Ultraviolet-inactivation of conidia from heterokaryons of Neurospora crassa containing UV-sensitive mutations, Mutat. Res., 27 (1975) 45–58.PubMedCrossRefGoogle Scholar
  25. 25.
    Stadler, D. R., and D. A. Smith, A new mutation in Neurospora for sensitivity to ultraviolet, Can. J. Genet. Cytol., 10 (1968) 916–919.PubMedGoogle Scholar
  26. 26.
    Tuveson, R. W., Genetic and enzymatic analysis of a gene controlling UV sensitivity in Neurospora crassa, Mutat. Res., 15 (1972) 411–424.PubMedCrossRefGoogle Scholar
  27. 27.
    Weijer, J., and N. V. Vigfussion, Sexuality in Neurospora crassa, I. Mutations to male sterility, Genet. Res., 19 (1972) 191–204.PubMedCrossRefGoogle Scholar
  28. 28.
    Worthy, T. E., and J. L. Epler, Biochemical basis of radiation sensitivity in mutants of Neurospora crassa, Mutat. Res., 19 (1973) 167–173.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Alice L. Schroeder
    • 1
  • Loree D. Olson
    • 1
  1. 1.Program in GeneticsWashington State UniversityPullmanUSA

Personalised recommendations