DNA Repair in Nuclei Isolated from Hela Cells
We have studied the DNA repair synthesis in HeLa cell isolated nuclei in presence of aphidicolin, which selectively inhibits DNA polymerase α but not DNA polymerases β and γ. The drug strongly depresses the DNA synthesis rate both in vivo and in the subcellular system but seems not to interfere with DNA repair. The enhancement in nucleotide incorporation by isolated nuclei after UV-irradiation is observed only in the presence of ATP.
KeywordsHeLa Cell Hairy Cell Leukemia Nucleotide Incorporation Control Nucleus Hairy Cell Leukemia Patient
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