DNA Repair in Nuclei Isolated from Hela Cells

  • Umberto Bertazzoni
  • Carmen Attolini
  • Miria Stefanini
Part of the Basic Life Sciences book series (BLSC, volume 15)


We have studied the DNA repair synthesis in HeLa cell isolated nuclei in presence of aphidicolin, which selectively inhibits DNA polymerase α but not DNA polymerases β and γ. The drug strongly depresses the DNA synthesis rate both in vivo and in the subcellular system but seems not to interfere with DNA repair. The enhancement in nucleotide incorporation by isolated nuclei after UV-irradiation is observed only in the presence of ATP.


HeLa Cell Hairy Cell Leukemia Nucleotide Incorporation Control Nucleus Hairy Cell Leukemia Patient 
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Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Umberto Bertazzoni
    • 1
  • Carmen Attolini
    • 1
  • Miria Stefanini
    • 1
  1. 1.Laboratorio CNR di Genetica Biochimica ed Evoluzionistica and Istituto di Geneticadell’Universita’ di PaviaPaviaItaly

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