Mutation Induction in a Radiation-Sensitive Variant of Mammalian Cells

  • Koki Sato
Part of the Basic Life Sciences book series (BLSC, volume 15)


Ultraviolet light (UV)-induced mutations were compared between a UV-sensitive variant and its parental mouse lymphoma cell line. The variant was originally isolated for its sensitivity to 4-nitroquinoline-1-oxide by employing a replica plating method and proved to be sensitive to the killing by UV as well. Caffeine potentiation of UV killing was observed to a similar extent in the variant and the parental cells, indicating that the caffeine-insensitive process is responsible for UV sensitivity in the variant. The induced mutation frequency as determined by resistance to 6-thioguanine was higher in the variant than in the parental cells per unit dose of UV as well as at the comparable survival level.


Parental Cell Xeroderma Pigmentosum Ataxia Telangiectasia Replica Plating Postreplication Repair 
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Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Koki Sato
    • 1
  1. 1.Division of GeneticsNational Institute of Radiological SciencesChiba 260Japan

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