Ultraviolet Light Induction of Diphtheria Toxin-Resistant Mutations in Normal and DNA Repair-Deficient Human and Chinese Hamster Fibroblasts

  • James E. Trosko
  • Roger S. Schultz
  • C. C. Chang
  • Tom Glover
Part of the Basic Life Sciences book series (BLSC, volume 15)


The role of unrepaired DNA lesions in the production of mutations is suspected of contributing to the initiation phase of carcinogenesis. Since the molecular basis of mutagenesis is not understood in eukaryotic cells, development of new genetic markers for quantitative in vitro measurement of mutations for mammalian cells is needed. Furthermore, mammalian cells, genetically deficient for various DNA repair enzymes, will be needed to study the role of unrepaired DNA lesions in mutagenesis. The results in this report relate to preliminary attempts (1) to characterize the diphtheria toxin resistance marker as a useful quantitative genetic marker in human cells and (2) to isolate and characterize various DNA repair-deficient Chinese hamster cells.


Excision Repair Diphtheria Toxin Chemical Carcinogen Xeroderma Pigmentosum Chinese Hamster Cell 


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Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • James E. Trosko
    • 1
  • Roger S. Schultz
    • 1
  • C. C. Chang
    • 1
  • Tom Glover
    • 1
  1. 1.Department of Human Development, College of Human MedicineMichigan State UniversityEast LansingUSA

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