Biochemical Characterization of Repair-Deficient Mutants of Drosophila
Excision Repair — mei-9, mus (2)201, mus (2)205, mus(3)308
Postreplication Repair — mei-41, mus(1)101, mus(1)104, mus (2)205, mus (3)302, mus (3)310, mus (3)311
DNA Synthesis — mus(1)101, mus(1)104, mus (2)205, mus (3)307, mus (3)308
The pleiotropic effect of several of these mutants indicates that their wild type alleles normally participate in more than one of these processes. Since mutants in at least three of these classes [mei-9, mei-41, mus(1)101] also alter meiotic recombination, their biochemical analysis in the more plentiful somatic cells can potentially reveal the function of the normal alleles in the rare meiotic tissues. A survey of the mutant properties is presented in Table 3.
The existence of a photorepair system has been documented in Drosophila, although no genetic blocks in that process have been identified. Identification of an AP-endonuclease activity suggests that Drosophila may possess a base excision mechanism. A technical advance is also described which will permit biochemical analysis of stocks in which homozygous females are sterile.
KeywordsExcision Repair Meiotic Recombination Pyrimidine Dimer Homozygous Female Brain Ganglion
Unable to display preview. Download preview PDF.
- 1.Baker, B. S., Effects of repair-deficient loci on mitotic and meiotic chromosome behavior, this volume.Google Scholar
- 3.Boyd, J. B., DNA repair in Drosophila, In: DNA Repair Mechanisms, P. C. Hanawalt, E. C. Friedberg and C. R. Fox (Eds.), Academic Press, New York, 1978, pp. 449–452.Google Scholar
- 4.Boyd, J. B., unpublished observations.Google Scholar
- 6.Boyd, J. B., and P. V. Harris, Excision repair in Drosophila: Analysis of strand breaks appearing in DNA of mei-9 cells following exposure to UV and N-acetoxy-N-acetyl-2-amino-fluorene, in preparation.Google Scholar
- 10.Boyd, S., unpublished observations.Google Scholar
- 13.Friedberg, E. C., T. Bonura, R. Cone, R. Simmons, and C. Anderson, Base excision repair of DNA, In: DNA Repair Mechanisms, P. C. Hanawalt, E. C. Friedberg and C. R. Fox (Eds.), Academic Press, New York, 1978, pp. 163–173.Google Scholar
- 14.Green and Smith, unpublished observations.Google Scholar
- 16.Harris, P. V., and J. B. Boyd, in preparation.Google Scholar
- 17.Harris, P. V., K. E. Smith, and J. B. Boyd, in preparation.Google Scholar
- 18.Hanawalt, P. C., E. C. Friedberg and C. R. Fox, (Eds.), DNA Repair, Academic Press, New York, 1978.Google Scholar
- 21.Meyer, H. U., Photoreactivation of ultraviolet mutagenesis in the polar cap of Drosophila (abstr.), Genetics, 36 (1951) 565.Google Scholar
- 23.Osgood, C., unpublished observations.Google Scholar
- 26.Sankaranarayanan, K., and F. Sobels, Radiation genetics, In: The Genetics and Biology of Drosophila, M. Ashburner and E. Novitski (Eds.), Academic Press, New York, 1976, Vol. 1c, pp. 1090–1250.Google Scholar
- 27.Smith, P. D., R. D. Snyder, and R. L. Dusenbery, Isolation and characterization of repair-deficient mutants of Drosophila melanogaster, This volume, P. 175.Google Scholar
- 29.Valencia, J. I., and W. Plaut, X-ray-induced DNA synthesis in polytene chromosomes (abstr.), J. Cell Biol., 43 (1969) 151a.Google Scholar
- 30.Würgler, F. E., and U. Graf, Mutation induction in repair-deficient strains of Drosophila, This volume, p. 223.Google Scholar