Effects of Recombination-Deficient and Repair-Deficient Loci on Meiotic and Mitotic Chromosome Behavior in Drosophila Melanogaster
The results of recent genetic and cytological studies on recombination-defective and repair-defective mutants of Drosophila melanogaster are summarized. These studies show that there is substantial overlap between the functions used in various aspects of DNA metabolism in Drosophila. Most loci first identified by either recombination-defective or mutagen-sensitive mutants have been shown also to function in nonmutagenized mitotic cells where their action is necessary to maintain the integrity of the genome: mutants at particular loci produce elevated frequencies of chromosome breakage, mitotic exchange, mutation, and/or chromosome loss.
Genetic studies of meiotic recombination show that many of the loci identified by recombination-defective mutants restrict where along the chromosome arms exchange may occur. Recent EM studies suggest that the products of at least some of these loci are components of recombination nodules. Region-specific control of DNA metabolism is also indicated by the finding of nonrandom patterns of chromosome breakage in some mutagen-sensitive mutants.
Recombination-defective mutants at two loci have been studied for their effects on sister chromatid exchanges (SCEs) and x-ray induced aberrations. Mutants at both loci are defective in steps necessary for the production of symmetrical chromatid interchanges but have little effect on SCEs.
KeywordsChromosome Aberration Synaptonemal Complex Sister Chromatid Exchange Meiotic Recombination Chromosome Breakage
Unable to display preview. Download preview PDF.
- 4.Baker, B. S., and J. C. Hall, Meiotic mutants: genetic control of meiotic recombination and chromosome segregation, In: The Genetics and Biology of Drosophila, Vol. la, M. Ashburner and E. Novitski, Eds., Academic Press, New York — London, 1976, pp. 350–434.Google Scholar
- 7.Baker, B. S., and D. A. Smith, In preparation.Google Scholar
- 11.Boyd, J. B., P. V. Harris, C. J. Osgood, and K. E. Smith, Biochemical characterization of repair-deficient mutants of Drosophila, This volume, p. 209.Google Scholar
- 17.Carpenter, A. T. C., and B. S. Baker, In preparation.Google Scholar
- 22.Gatti, M., S. Pimpinelli, and B. S. Baker, Relationships between chromatid interchanges, sister chromatid exchanges and meiotic recombination in Drosophila melanogaster, Proc. Natl. Acad. Sci. (U.S.), (1980), in press.Google Scholar
- 29.Lawlor, T. A., Genetic and cytological localization of mei-9, Drosophila Inform. Serv., in press.Google Scholar
- 31.Lewis, E. B., and W. Gencarella, Claret and nondisjunction in Drosophila melanogaster, Genetics (Abstr.) 37(1952) 600–601.Google Scholar
- 32.Lindsley, D. L., L. Sandler, B. Nicoletti, and G. Trippa, Mutants affecting meiosis in natural populations of Drosophila melanogaster, In: Replication and Recombination of Genetic Material, W. J. Peacock and R. D. Brock, Eds., Australian Acad. Sci., Canberra, 1968, pp. 253–267.Google Scholar
- 35.Mason, J. M., M. M. Green, K. E. Smith, and J. B. Boyd, Genetic analysis of X-linked mutagen-sensitive mutants of Drosophila melanogaster, submitted.Google Scholar
- 37.Nguyen, T. D., and J. B. Boyd, The mei-9 locus of Drosophila melanogaster is deficient in repair replication of DNA, Genetics, 83 (1976) s55.Google Scholar
- 43.Sandler, L., Induction of autosomal meiotic mutants by EMS in D. melanogaster, Drosophila Inform. Serv., 47 (1971) 68.Google Scholar
- 46.Smith, D. A., and B. S. Baker, In preparation.Google Scholar
- 49.Smith, P. D., Mutagen sensitivity of Drosophila melanogaster, IV. Interactions of X chromosome mutants, In: DNA Repair Mechanisms (ICN-UCLA Symposia on Molecular and Cellular Biology IX), P. C. Hanawalt, E. C. Friedberg, and C. F. Fox, Eds., Academic Press, New York, 1978, pp. 453–456.Google Scholar
- 51.Smith, P. D., R. D. Snyder, and R. L. Dusenbery, Isolation and characterization of repair-deficient mutants of Drosophila melanogaster, This volume.Google Scholar
- 52.Würgler, F. E., and U. Graf, Mutation induction in repair-deficient strains of Drosophila, This volume.Google Scholar