Isolation and Characterization of Repair-Deficient Mutants of Drosophila melanogaster

  • P. Dennis Smith
  • Ronald D. Snyder
  • Ruth L. Dusenbery
Part of the Basic Life Sciences book series (BLSC, volume 15)


Mutagen-sensitive mutants of Drosophila melanogaster are being utilized to determine the number and function of genes which control various aspects of DNA metabolism including replication, repair and recombination and to define the relationship of each of these functions to mutation production.

Nineteen loci which confer enhanced sensitivity to killing by methyl methanesulfonate have been detected on the X and second chromosomes. These loci can be grouped into five separate classes on the basis of cross-sensitivity to methyl methanesulfonate, x-ray, UV and nitrogen mustard. Some loci affect both somatic and meiotic functions, as assayed by mutagen sensitivity and recombination deficiency, while others affect only somatic functions.

Genetic methods have been employed to determine whether these mutants, which are able to survive in the absence of mutagen treatment, represent loci which have dispensable functions or leaky alleles of loci which have indispensable cellular functions. In several cases, both leaky and null alleles have been identified, indicating that at least some of these loci represent apparently dispensable cellular functions.

The mutagen sensitivity of doubly-mutant mus strains is being employed to assign mutants to common pathways for DNA repair in order to corroborate data obtained from biochemical studies and to explore whether a genetic ordering of metabolic steps in each repair pathway is possible. These data may be used in conjunction with the effects on the frequency and nature of mutations produced by various agents in each of the mutant strains to identify which of these pathways involve error-free or error-prone repair processes.


Nitrogen Mustard Mutagen Sensitivity Mutagen Treatment Methyl Methanesulfonate Mutation Production 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Baker, B. S., and A. T. C. Carpenter, Genetic analysis of sex chromosomal meiotic mutants in Drosophila melanogaster, Genetics, 71 (1972) 255–286.PubMedGoogle Scholar
  2. 2.
    Baker, B. S., A. T. C. Carpenter, and P. Ripoll, The utilization during mitotic cell division of loci controlling meiotic recombination and disjunction in Drosophila melanogaster, Genetics, 90 (1978) 531–578.PubMedGoogle Scholar
  3. 3.
    Baker, B. S., and D. A. Smith, The effects of mutagen-sensitive mutants of Drosophila melanogaster in non-mutagenized cells, Genetics, in press.Google Scholar
  4. 4.
    Boyd, J. B., M. D. Golino, T. D. Nguyen, and M. M. Green, Isolation and characterization of X-linked mutants of Drosophila melanogaster which are sensitive to mutagens, Genetics, 84 (1976) 485–506.PubMedGoogle Scholar
  5. 5.
    Boyd, J. B., M. D. Golino, and R. B. Setlow, The mei-9a mutant of Drosophila melanogaster increases mutagen sensitivity a and decreases excision repair, Genetics, 84 (1976) 527–544.PubMedGoogle Scholar
  6. 6.
    Boyd, J. B., and R. B. Setlow, Characterization of postreplication repair in mutagen sensitive strains of Drosophila melanogaster, Genetics, 84 (1976) 507–526.PubMedGoogle Scholar
  7. 7.
    Clark, A. J., and M. R. Volkert, A new classification of pathways repairing pyrimidine dimer damage in DNA. In: DNA Repair Mechanisms, P. C. Hanawalt, E. C. Friedberg, and C. F. Fox (Eds.), Academic Press, New York, 1978, pp. 57-57–72.Google Scholar
  8. 8.
    de Serres, F. J., Mutation induction in repair-deficient strains of Neurospora, This volume, p. 75.Google Scholar
  9. 9.
    Game, J. C., and B. S. Cox, Epistatic interactions between four rad loci in yeast, Mutat. Res., 16 (1972) 353–362.PubMedCrossRefGoogle Scholar
  10. 10.
    Graf, U., and F. E. Würgler, Mutagen sensitive mutants in Drosophila: relative MMS sensitivity and maternal effects, Mutat. Res., 52 (1978) 381–394.CrossRefGoogle Scholar
  11. 11.
    Graf, U., E. Vogel, U. P. Biber, and F. E. Würgler, Genetic control of mutagen sensitivity in Drosophila melanogaster. A new allele at the mei-9 locus on the X-chromosome, Mutat. Res., 59 (1979) 129–133.PubMedCrossRefGoogle Scholar
  12. 12.
    Haynes, R. H., DNA repair and the genetic control of radiosensitivity in yeast. In: Molecular Mechanisms for Repair of DNA, Part B, P. C. Hanawalt and R. B. Setlow (Eds.), Plenum Press, New York-London, 1975, pp. 529–540.Google Scholar
  13. 13.
    Haynes, R. H., Workshop summary: DNA repair in lower eukaryote eukaryotes. In: DNA Repair Mechanisms, P. C. Hanawalt, E. C. Friedberg, and C. F. Fox (Eds.), Academic Press, New York, 1978, pp. 405–411.Google Scholar
  14. 14.
    Kimball, R. F., Relationship between repair processes and mutation induction in bacteria, This volume, p. 1.Google Scholar
  15. 15.
    Lawrence, C., and R. B. Christensen, Ultraviolet light induced mutagenesis in Saccharomyces cerevisiae. In: DNA Repair Mechanisms, P. C. Hanawalt, E. C. Friedberg, and C. F. Fox (Eds.), Academic Press, New York, pp. 437-440.Google Scholar
  16. 16.
    Lawlor, T., personal communication.Google Scholar
  17. 17.
    Mason, J., personal communication.Google Scholar
  18. 18.
    Morimyo, M., and Y. Shimazu, Evidence that gene uvrB is indispensable for a polymerase-I deficient strain of E. coli, Molec. Gen. Genet., 147 (1976) 243–250.PubMedCrossRefGoogle Scholar
  19. 19.
    Prakash, L., The relation between repair of DNA and radiation and chemical mutagenesis in Saccharomyces cerevisiae. Mutat. Res., 41 (1976) 241–248.PubMedCrossRefGoogle Scholar
  20. 20.
    Prakash, L. and S. Prakash, Isolation and characterization of MMS-sensitive mutants of Saccharomyces cerevisiae. Genetics, 86 (1977) 33–55.PubMedGoogle Scholar
  21. 21.
    Prakash, L. and S. Prakash, Pathways of DNA repair in yeast. In: DNA Repair Mechanisms, P. C. Hanawalt, E. C. Friedberg and C. F. Fox (Eds.), Academic Press, New York, 1978, pp. 413–416.Google Scholar
  22. 22.
    Prakash, L., and S. Prakash, Genetic analysis of error-prone repair systems in Saccharomyces cerevisiae, This volume, 141.Google Scholar
  23. 23.
    Samson, L., and J. Cairns, A new pathway of DNA repair in Escherichia coli, Nature, 267 (1977) 281–282.PubMedCrossRefGoogle Scholar
  24. 24.
    Smith, K. C., Multiple pathways of DNA repair in bacteria and their role in mutagenesis, Photochem. Photobiol., 28 (1978) 121–129.PubMedCrossRefGoogle Scholar
  25. 25.
    Smith, P. D., Mutagen sensitivity of Drosophila melanogaster. I. Isolation and preliminary characterization of a methyl methanesulfonate-sensitive strain, Mutat. Res., 20 (1973) 215–220.PubMedCrossRefGoogle Scholar
  26. 26.
    Smith, P. D., and C. G. Shear, X-ray and ultraviolet light sensitivities of a methyl methanesulfonate-sensitive strain of Drosophila melanogaster, In: Mechanisms in Recombination, R. F. Grell (Ed.), Plenum Press, New York-London, 1974, pp. 399–403.CrossRefGoogle Scholar
  27. 27.
    Smith, P. D., Mutagen sensitivity of Drosophila melanogaster. III. X-linked loci governing sensitivity to methyl methanesulfonate, Molec. Gen. Genet., 149 (1976) 73–85.PubMedCrossRefGoogle Scholar
  28. 28.
    Snyder, R. D., unpublished data.Google Scholar
  29. 29.
    Snyder, R. D. and P. D. Smith, in preparation.Google Scholar
  30. 30.
    Witkin, E. M., Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli, Bacteriol. Rev., 40 (1976) 869–907.PubMedGoogle Scholar
  31. 31.
    Yamamoto, Y., M. Katsuki, M. Sekiguchi, and N. Otsuji, Escherichia coli gene that controls sensitivity to alkylating agents, J. Bacteriol., 135 (1978) 144–152.PubMedGoogle Scholar
  32. 32.
    Yamamoto, Y., and M. Sekiguchi, Pathways for repair of DNA damaged by alkylating agent in Escherichia coli, Molec. Gen. Genet., 171 (1979) 251–256.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • P. Dennis Smith
    • 1
  • Ronald D. Snyder
    • 1
  • Ruth L. Dusenbery
    • 1
  1. 1.Department of BiologyEmory UniversityAtlantaUSA

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