Correlation of Amino Acid Concentrations, Permeability and Structural Changes in Monkey Brain Following both Sustained and Transient Middle Cerebral Artery Occlusion

  • K. A. Conger
  • J. H. Garcia


The effect of ischemia on the extravasation of inulin was determined following acute middle cerebral artery (MCA) occlusion by comparing amounts of inulin present in ischemic areas of rhesus monkey brain to values obtained from non-ischemic areas. The degree of ischemic damage present was evaluated by both biochemical and structural methods. Increased alanine to glutamate (A:G) ratios (a biochemical index of ischemia) correlated well with increased extravasation of inulin. In a separate study, transient ischemia was studied in monkeys whose MCA was occluded for one hour followed by one week’s reperfusion. Throughout the study, local cerebral blood flow (CBF) was monitored. Following a week of reperfusion, no differences in sorbitol or inulin compartments were noted between hemispheres; in addition, A:G ratios had returned to preocclusive levels. These results are interpreted as evidence of blood-brain barrier (BBB) repair and brain metabolic recovery. Light microscopic demonstration of heterogeneous vacuolation correlated well with a biochemical permeability index (sorbitol:inulin ratio) calculated after equilibration of tissue slices in sorbitol and inulin. Equilibrium marker measurements may be useful adjuncts to light and electron microscopic evaluation of ischemic injury.


Middle Cerebral Artery Occlusion Caudate Nucleus Brain Edema Amino Acid Concentration Insular Cortex 
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Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • K. A. Conger
    • 1
  • J. H. Garcia
    • 1
  1. 1.Department of PathologyUniversity of Alabama Medical CenterBirminghamUSA

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