Neuronal-Glial Interactions during Neural Growth in Culture

  • Antonia Vernadakis


Although there exists a vast body of information concerning brain maturation, we know relatively very little about neuronal-glial interrelationships during neural growth and differentiation. In view of the fact that glial cells in the central nervous system (CNS) outnumber neurons by a factor of ten, their role in CNS function continues to be the subject of much investigation. Glial cells have been proposed to be involved in myelination (3) to act as spatial ionic buffers (10), to be electrically coupled to neurons (23), and to proliferate with increased neuronal activity (4, 11). More recently we (22), and others (6), have proposed that glial cells may also modulate neuronal activity by regulating the amount of neurotransmitter substances in the microenvironment. In this paper we will review and discuss responses of glial cells to hormones and glial cell-hormone-neurotransmitter interrelationship.


Glial Cell Chick Embryo Cerebral Hemisphere Central Nervous System Function Neural Growth 
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  1. 1.
    Arnold, E.B. and Vernadakis, A. (1979): Development of tyrosine hydroxylase activity in dossociated cerebral cell cultures. Develop. Neurosci. 2: 46–50.CrossRefGoogle Scholar
  2. 2.
    Booher, J. and Sensenbrenner, M. (1972): Growth and cultivation of dissociated neurons and glial cells from embryonic chick, rat and human brain in flask cultures. Neurobiology 2: 97–105.PubMedGoogle Scholar
  3. 3.
    Bunge, R.P. (1968): Glial cells and the central myelin sheath. Physiol. Rev. 48: 197–251.PubMedGoogle Scholar
  4. 4.
    Diamond, M.C., Law, F., Rhodes, H., Lidner, B., Rosenzeig, M.R., Krech, D. and Bennett, E.L. (1966): Increases in cortical depth and glial numbers in rats subjected to enriched environment. J. Comp. Neurol. 128: 117–126.PubMedCrossRefGoogle Scholar
  5. 5.
    Hanaway, J. (1967): Formation and differentiation of external layer of the chick cerebellum. J. Comp. Neurol. 131: 1–14.PubMedCrossRefGoogle Scholar
  6. 6.
    Henn, F.A. and Hamberger, A. (1971): Glial cell function uptake of transmitter substances. Proc. Nat. Acad. Sci., USA 68: 2686–2690.CrossRefGoogle Scholar
  7. 7.
    Iversen, L.L. (1971): Role of transmitter uptake mechanisms in synaptic neurotransmission. Brit. J. Pharmacol. 4: 571–591.Google Scholar
  8. 8.
    Iversen, L.L., and Salt, P.J. (1970): Inhibition of catecholamine uptake2 by steroids in the isolated heart. Brit. J. Pharmacol. 4: 571–591.Google Scholar
  9. 9.
    Kellogg, C., Vernadakis, A. and Rutledge, C.O. (1971): Uptake and metabolism of 3H-norepinephrine in the cerebral hemispheres of chick embryos. J. Neurochem. 18: 1931–1938.PubMedCrossRefGoogle Scholar
  10. 10.
    Kuffler, S.W. (1967): Neuroglial cells: physiological properties and a potassium mediated effect of neuronal activity on the glial membrane potential. Proc. Roy. Soc. (London) B. 168: 1–21.CrossRefGoogle Scholar
  11. 11.
    Kuffler, S.W. and Nicholls, J.G. (1966): The physiology of neuroglial cells. Ergeb. Physiol. 57: 1–90.PubMedGoogle Scholar
  12. 12.
    Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951): Protein measurement with the tolin phenol reagent. J. Biol. Chem. 193: 265–275.PubMedGoogle Scholar
  13. 13.
    McEwen, B.S., Weiss, J.M. and Schwartz, L.S. (1969): Uptake of corticosterone by rat brain and its concentration by certain limbic structures. Brain Res. 16: 227–241.PubMedCrossRefGoogle Scholar
  14. 14.
    Sensenbrenner, M., Booher, J. and Mandel, P. (1971): Cultivation and growth of dissociated neurons from chick embryo cerebral cortex in the presence of different substrates. Z. Zellforsch. 117: 559–569.PubMedCrossRefGoogle Scholar
  15. 15.
    Vernadakis, A. (1971): Hormonal factors in the proliferation of glial cells in culture. In: Influence of Hormones on the Nervous System, D.H. Ford (ed.), Proc. Int. Soc. of Psychoneuroendocrinology, Brooklyn, New York, 1970, S. Karger, Basel.Google Scholar
  16. 16.
    Vernadakis, A. (1973): Uptake of 33H-norepinephrine in the cerebral hemispheres and cerebellum of the chicken throughout the lifespan. Mech. Age. Dev. 2: 371–379.CrossRefGoogle Scholar
  17. 17.
    Vernadakis, A. (1973): Changes in nucleic acid content and butyrylcholinesterase activity in CNS structures during the life span of the chicken. J. Gerontol. 28: 281–286.PubMedGoogle Scholar
  18. 18.
    Vernadakis, A. (1974): Neurotransmission: A proposed mechanism of steroid hormones in the regulation of brain function. In: Proceedings of the Mie Conference of the International Society for Psychoneuroendocrinology, N. Hatotani (ed.), S. Karger AG, Basel.Google Scholar
  19. 19.
    Vernadakis, A. and Culver, B. (1979): Neural tissue culture: A biochemical tool. In: The Biochemistry of Brain, S. Kumar (ed.), Pergamon Press (in press).Google Scholar
  20. 20.
    Vernadakis, A. and Nidess, R. (1976): Biochemical characteristics of C-6 glial cells. Neurochem. Res. 1: 385–402.CrossRefGoogle Scholar
  21. 21.
    Vernadakis, A., Culver, B. and Nidess, R. (1978): Actions of steroid hormones on neural growth in culture: Role of glial cells. In: Proceedings of the 7th International Congress of the International Society for Psychoneuroendocrinology, Psychoneuroendocrinology 3: 47–64.Google Scholar
  22. 22.
    Walker, F.D. and Hild, W.J. (1969): Neuroglia electrically coupled to neurons. Science 165: 602–603.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Antonia Vernadakis
    • 1
  1. 1.Departments of Psychiatry and PharmacologyUniversity of Colorado School of MedicineDenverUSA

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