Advertisement

Metabolic Patterns in Cerebral Capillaries

  • B. B. Mršulja
  • B. M. Djuričić
  • D. V. Micic

Abstract

Brain capillaries and parenchyma are morphologically and metabolically separate and distinct compartments; the function of endothelial cells in transporting substances from blood to brain requires some specialized properties. The simplicity in isolating cerebral capillaries permits the investigation on their biochemical properties. Such an approach has revealed that the enzymatic organization of brain capillaries differs from that of the parenchyma (4–7, 11, 15). The brain capillaries which we have been studying are most probably a part of the blood-brain barrier (BBB); hence it is tentatively proposed that the specific biochemical organization of the brain microvasculature regulates the transport of substrates across the blood to the brain.

Keywords

Amino Acid Transport Neutral Amino Acid Metabolic Pattern Cerebral Capillary Glycogen Synthetase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Abe, T., Abe, K3, Swink, M.E., Klatzo, I. and Spatz, M. (1978): The H-norepinephrine uptake and fate in the isolated cerebral capillaries. Soc. Neuroscience, 8th Ann. Meet. (Abstracts), Vol. 4, p. 441.Google Scholar
  2. 2.
    Bertler, A., Falck, B., Owman, Ch. and Rosengren, E. (1966): The localization of monoaminergic blood-brain barrier mechanism. Pharmacol. Rev. 18: 369–385.PubMedGoogle Scholar
  3. 3.
    Christensen, H.N., Aspen, A.J. and Rice, E.G. (1956): Metabolism in the rat of three amino acids lacking alpha-hydrogen. J. Biol. Chem. 220: 287–294.PubMedGoogle Scholar
  4. 4.
    Djuricié, B.M. and Mrsulja, B.B. (1977): Enzymatic activity of the brain: microvessels vs. total brain homogenate. Brain Res. 138: 561–564.CrossRefGoogle Scholar
  5. 5.
    Diuricié, B.M. and Mrsulja, B.B. (1979): Brain micro-vessel hexokinase: kinetic properties. Experientia 35: 169–171.CrossRefGoogle Scholar
  6. 6.
    Diuricié, B.M., Rogac, Lj., Spatz, M., Rakié, Lj.M. and Mrsulja, B.B. (1978): Brain microvessels. I. Enzymic activities. In: Adv. Neurology, Vol. 20, J. Cervos-Navarro, et al. (eds.), pp. 197–205, Raven Press, New York.Google Scholar
  7. 7.
    Goldstein, G.W., Wolinsky, J.S., Csejtey, J. and Diamond, I. (1975): Isolation of metabolically active capillaries from rat brain. J. Neurochem. 25: 715–717.PubMedCrossRefGoogle Scholar
  8. 8.
    Hardebo, J.E., Edvinson, L., Falck, B., Lindvall, M., Owmen, Ch., Rosengren, E. and Svengaard, N.A. (1976): Experimental models for histochemical and chemical studies of the enzymatic blood-brain harrier for amine precursors. In: The Cerebral Vessel Wall, J. Cervos-Navarro et al., (eds.), pp. 233–244, Raven Press, New York.Google Scholar
  9. 9.
    Kaplan, G.P., Hartman, B.K. and Creveling, C.R. (1979): Immunohistochemical demonstration of catechol-O-methyl transferase in mammalian brain. Brain Res. 167: 241–250.PubMedCrossRefGoogle Scholar
  10. 10.
    Miéic, D.V., Swink, M., Midid, J., Klatzo, I. and Spatz, M. (1979): The ischemic and postischemic effects on the uptake of neutral amino acids in isolated cerebral capillaries. Experientia 35: 625–626.CrossRefGoogle Scholar
  11. 11.
    Mrsulja, B.B. and Djuricie, B.M. (1979): Biochemical characteristics of cerebral capillaries. Symposium: The Cerebral Microvasculature, Galveston (in press).Google Scholar
  12. 12.
    Mrsulja, B.B., Mrsulja, B.J., Fujimoto, T., Klatzo, I. and Spatz, M. (1976): Isolation of brain capillaries: a simplified technique. Brain Res. 110: 361–365.PubMedCrossRefGoogle Scholar
  13. 13.
    Orlowski, M. (1976): Possible role of glutathione in transport processes. In: Transport Phenomena in the Nervous System, G. Levy et al., (eds.) pp. 13–28, Plenum Press, New York.Google Scholar
  14. 14.
    Rapoport, S.I. (1976): Blood-Brain Barrier in Physiology and Medicine, Raven Press, New York.Google Scholar
  15. 15.
    Sessa, G., Orlowski, M. and Green, J.P. (1976): Isolation from bovine brain of a fraction containing capillaries and fraction containing membrane fragments of the choroid plexus. J. Neurobiol. 7: 51–61.PubMedCrossRefGoogle Scholar
  16. 16.
    Spatz, M., Midid, D.V., Fujimoto, T., Mrsulja, B.B. and Klatzo, I. (1979): Transport phenomena in cerebral ischemia. In: Pathophysiology of Cerebral Energy Metabolism. B.B. Mrsulja, et al., (eds.) pp. 143–153, Plenum Press, New York.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • B. B. Mršulja
    • 1
  • B. M. Djuričić
    • 1
  • D. V. Micic
    • 1
  1. 1.Laboratory for Neurochemistry, Institute of BiochemistryFaculty of MedicineBelgradeYugoslavia

Personalised recommendations