Profiles of Target-Cell Prolactin and Adrenocorticotropin during Lactational Diestrus

  • Janet M. Nolin
Part of the Biochemical Endocrinology book series (BIOEND)


Endocrinology can be viewed as a very old science when one considers that ablation of certain of the glands of internal secretion, in particular the gonads, was practiced very early on in our history. (One can even postulate that the bravest among some of our ancestors got hold of a saber-toothed tiger long enough to turn him into a pussycat!) It was not until whole organs, homogenates, or extracts were tested for activity that could repair a deficiency, however, that modern endocrinology began about a century ago. This sort of qualitative bioassay approach continued to be used well past the first third of the present century and involved hormones exclusively in their glands of origin, i.e., hormones in very large amounts. As bioassay sensitivities were improved, detection of a relatively wide range of concentrations became possible in the late 1940’s and 1950’s, and during this period it even became possible to detect some hormones in transit to their targets, but again, the amounts that could be detected were present at the lower concentrations that could be measured in tissues of origin, and therefore the improved methods could detect hormones in blood only if they were present in supraphysiological amounts. Then, in the late 1960’s and early 1970’s, came radioimmunoassays (RIAs) and radioreceptor assays (RRAs) that allowed the study of hormones in transit, not only in physiological, but also in subphysiological, concentrations.


Mammary Gland Granulosa Cell Adrenal Cortex Activity Cycle Follicular Fluid 


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  1. Astwood, E.B., 1941, The regulation of corpus luteum function by hypophysial luteotrophin, Endocrinology 28: 309.CrossRefGoogle Scholar
  2. Baker, B.L., 1970, Studies on hormone localization with emphasis on the hypophysis, J. Histochem. Cytochem. 18: 1.CrossRefGoogle Scholar
  3. Baker, T.G., and Hunter, R.H.F., 1978, Interrelationships between the oocyte and somatic cells within the Graafian follicle of mammals. Ann. Biol. Anim. Biochem. Biophys. 18: 419.CrossRefGoogle Scholar
  4. Channing, C.Y., 1979, Intraovarian inhibitors of follicular function, in: Ovarian Follicular Development and Function ( A.R. Midgley and W.A. Sadler, eds.), pp. 59–64, Raven Press, New York.Google Scholar
  5. Crisp T.M., 1979, Studies on the binding of labelled prolactin (PRL) to rat granulosa cell cultures, Anat. Rec. 193: 512 (abstract).Google Scholar
  6. Davis, J.O., 1975, Regulation of aldosterone secretion, in: Handbook of Physiology, Section 7, Endocrinology, Vol. VI, Adrenal Gland (R.O. Greep and E.B. Astwood, section eds.), pp. 77–106, American Physiological Society, Washington, D.C.Google Scholar
  7. Dornhorst, A., and Gann, D.S., 1978, Immunoperoxidase stains cortisol in adrenal and pituitary, J. Histochem. Cvtochem. 26: 909.CrossRefGoogle Scholar
  8. Grosvenor, C.E., Mena, F., Dhariwal, A.Y.S., and McCann, S.M., 1967, Reduction of milk secretion by prolactin-inhibiting factor: Further evidence that exteroceptive stimuli can release pituitary prolactin in rats, Endocrinology 81: 1021.CrossRefGoogle Scholar
  9. Haynes, R.C., Jr., 1975, Theories on the mode of action of ACTH in stimulating secretory activity of the adrenal cortex, in: Handbook of Physiology, Section 7, Endocrinology, Vol. VI, Adrenal Gland (R.O. Greep and E.B. Astwood, section eds.), pp. 69–76, American Physiological Society, Washington, D.C.Google Scholar
  10. Hutson, J.C., Gardner, P.J., and Childs, M.G., 1977, Immunocytochemical localization of a follicle-stimulating hormone-like molecule in the testis, J. Histochem. Cytochem. 25: 1119.CrossRefGoogle Scholar
  11. Le Cam, A., Maxfield, F., Willingham, M., and Pastan, I., 1979, Insulin stimulation of amino acid transport in isolated rat hepatocytes is independent of hormone internalization, Biochem. Biophys. Res. Commun. 88: 873.CrossRefGoogle Scholar
  12. Lefkowitz, R.J., Roth, J., Pricer, W., and Pastan, 1., 1970, ACTH receptors in the adrenal: Specific binding of ACTH-1251 and its relation to adenyl cyclase, Proc. Natl. Acad. Sci. U.S.A. 65: 745.CrossRefGoogle Scholar
  13. McKerns, K.W., 1978, Regulation of gene expression in the nucleus by gonadotropins, in: Structure and Function of the Gonadotropins ( K.W. McKerns, ed.), pp. 315–338, Plenum Press, New York.Google Scholar
  14. McNatty, K.P., Hunter, W.M., McNeilly, A.S., and Sawers, R.S., 1975, Changes in the concentration of pituitary and steroid hormones in the follicular fluid of human Graafian follicles throughout the menstrual cycle, J. Endocrinol. 64: 555.CrossRefGoogle Scholar
  15. McNatty, K.P., McNeilly, A.S., and Sawers, R.S., 1977, Prolactin and progesterone secretion by human granulosa cells in vitro, in: Prolactin and Human Reproduction ( P.G. Crosignani and C. Robyn, eds.), pp. 109–117, Academic Press, New York.Google Scholar
  16. Midgley, A.R., Jr., 1972, Gonadotropin binding to frozen sections of ovarian tissue, in: Gonadotropins ( B.B. Saxena, C.G. Beling, and H.M. Gandy, eds.), pp. 248–260, John Wiley, New York.Google Scholar
  17. Nakane, P.K., 1968, Simultaneous localization of multiple tissue antigens using the peroxidase-labeled antibody method: A study on pituitary glands of the rat, J. Histochem. Cytochem. 16: 557.CrossRefGoogle Scholar
  18. Nekola, M.V., and Nalbandov, A.V., 1971, Morphological changes of rat follicular cells as influenced by occytes, Biol. Reprod. 4: 154.Google Scholar
  19. Nolin, J.M., 1978a, Intracellular prolactin in rat corpus luteum and adrenal cortex, Endocrinology 102: 402.CrossRefGoogle Scholar
  20. Nolin, J.M., 1978b, “Anticipant” receptors—a concept based on comparisons between endogenous PRL and free PRL-binding sites in individual milk secretory cells, Fed. Proc. Fed. Am. Soc. Exp. Biol. 37:265 (Abstract 284).Google Scholar
  21. Nolin, J.M., 1978c, Apparent incorporation of endogenous prolactin by dictyate mammalian oocytes, J. Cell Biol. 79: 177a (Abstract G 1007).Google Scholar
  22. Nolin, J.M., 1978d, Target cell prolactin, in: Structure and Function of Gonadotropins ( K.W. McKerns, ed.), pp. 151–182, Plenum Press, New York.Google Scholar
  23. Nolin, J.M., 1979a, Intracellular sites of action for endogenous ACTH?, Proc. Endocr. Soc., p. 135 (Abstract 249).Google Scholar
  24. Nolin, J.M., 1979b, The prolactin incorporation cycle of the milk secretory cell: An integral component of the prolactin response cycle, J. Histochem. Cytochem. 27: 1203.CrossRefGoogle Scholar
  25. Nolin, J.M., 1979e, Does PRL inhibit oocyte maturation? Undergo transcellular transport by granulosa cells (GC)? Act directly on GC nuclei?, Biol. Reprod. 20: 66a (Abstract 109).Google Scholar
  26. Nolin, J.M., 1980a, Incorporation of endogenous prolactin by granulosa cells and dictyate oocytes in the postpartum rat: Effects of estrogen, Biol. Reprod. 22: 417.Google Scholar
  27. Nolin, J.M., 19806, Incorporation of regulatory peptide hormones by individual cells of the adrenal cortex: Prolactin-adrenocorticotrophin differences, Peptides 1:249.Google Scholar
  28. Nolin, J.M., and Bogdanove, E.M., 1980, Effects of estrogen on prolactin (PRL) incorporation by lutein and milk secretory cells and on pituitary PRL secretion in the postpartum rat: Correlations with target cell responsiveness to PRL, Biol. Reprod. 22: 393.Google Scholar
  29. Nolin, J.M., and Witorsch, R.J., 1976, Detection of endogenous immunoreactive prolactin in rat mammary epithelial cells during lactation, Endocrinology 99: 949.CrossRefGoogle Scholar
  30. Ogle, T.F., and Kitay, J.I., 1979, Interactions of prolactin and adrenocorticotropin in the regulation of adrenocortical secretions in female rats, Endocrinology 104: 40.CrossRefGoogle Scholar
  31. Peaker, M., 1977, The aqueous phase of milk: Ion and water transport, in: Comparative Google Scholar
  32. Aspects of Lactation (M. Peaker, ed.), pp. 113–134, Academic Press, New York.Google Scholar
  33. Rolland, R., and Hammond, J.M., 1975, Demonstration of a specific receptor for prolactin in porcine granulosa cells, Endocr. Res. Commun. 2: 281.CrossRefGoogle Scholar
  34. Rubin, R.Y., and Laychock, S.G., 1978, Prostaglandins and calcium—membrane interactions in secretory glands, Ann. N.Y. Acad. Sci. 307: 377.CrossRefGoogle Scholar
  35. Saacke, R.G., and Heald, C.W., 1974, Cytological aspects of milk formation and secretion, in: Lactation Ll ( B.L. Larson and V.R. Smith, eds.), pp. 147–189, Academic Press, New York.Google Scholar
  36. Schuetz, A.W., 1974, Role of hormones in oocyte maturation, Biol. Reprod. 10:150. Sternberger, L.A., Petrali, J.P., Joseph, S.A., Meyer, H.G., and Mills, K.R., 1978Google Scholar
  37. Specificity of the immunocytochemical luteinizing hormone-releasing hormone receptorGoogle Scholar
  38. reaction, Endocrinology 102:63.Google Scholar
  39. Szego, C.M., Rakich, D.R., Seder, B.J., and Gross, R.S., 1974, Lysosomal labilization: Rapid, target-specific effect of ACTH, Endocrinology 95: 863.CrossRefGoogle Scholar
  40. Szöllösi, D., 1972, Changes of some cell organelles during oogenesis in mammals, in: Oogenesis ( J.D. Biggers and A.W. Schuetz, eds.), pp. 47–64, University Park Press, Baltimore.Google Scholar
  41. Thatcher, W.W., Wilcox, C.J., Bazer, F.W., Collier, R.J., Eley, R.M., Stover, D.G., and Bartol, F.F., 1979, Bovine conceptus effects prepartum and potential carryover effects postpartum, Proceedings of the Beltsville Symposium on Animal Reproduction, May 14–17, 1978, Beltsville, Maryland.Google Scholar
  42. Topper, Y.J., 1970, Multiple hormone interactions in the development of mammary gland in vitro, in: Recent Progress in Hormone Research ( E.B. Astwood, ed.), pp. 287–308, Academic Press, New York.Google Scholar
  43. Veldhuis, J.D., Klase, P.A., and Hammond, J.M., 1979, Prolactin induction or inhibition of steroidogenesis in cultured porcine granulosa cells, Biol. Reprod. 20: 70A (Abstract 118).Google Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Janet M. Nolin
    • 1
  1. 1.Department of PhysiologyMedical College of VirginiaRichmondUSA

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