Interactions among LH, FSH, PRL, LH-RH, and Sex Steroids in the Control of Testicular LH, FSH, and PRL Receptors in the Rat

  • P. A. Kelly
  • F. Labrie
  • C. Auclair
  • C. Séguin
  • A. Bélanger
  • M. R. Sairam
Part of the Biochemical Endocrinology book series (BIOEND)


Within the last several years, improved methods to label highly purified polypeptide hormones to a high level of specific activity, with a retention of biological activity, along with advances in subcellular-fractionation techniques have allowed an important increase in our understanding of the mechanisms of action of peptide hormones. Binding of hormones to receptors located in the plasma membrane is the first event in the action of these hormones in their target tissues, and specific receptors for a large number of hormones have been identified (Kahn, 1976).


Luteinizing Hormone Leydig Cell Testis Weight Luteinizing Hormone Receptor Dwarf Mouse 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Aragona, C., Bohnet, H.G., and Friesen, H.G., 1977, Localization of prolactin binding in prostate and testis: The role of serum Prolactin concentrations on the testicular LH receptor, Acta Endocrinol. 84: 402.Google Scholar
  2. Arimura, A., Serafini, P., Talbot, S., and Schally, A.V., 1979, Reduction of testicular luteinizing hormone/human chorionic gonadotropin receptors by [O-Trpl-luteinizing hormone-releasing hormone in hypophysectomized rats, Biochem. Biophys. Res. Commun. 90: 687.CrossRefGoogle Scholar
  3. Auclair, C., Kelly, P.A., Coy, D.H., Schally, A.V., and Labrie, F., 1977a, Potent inhibitory activity of [D-Leu6,des-Gly-NH_“’]LHRH ethylamide on LH/hCG and PRL testicular receptor levels in the rat, Endocrinology. 101:1890CrossRefGoogle Scholar
  4. Auclair, C., Kelly, P.A., Labrie, F., Coy, D.H., and Schally, A.V., 19776, Inhibition of testicular luteinizing hormone receptor level by treatment with a potent luteinizing hormone-releasing hormone agonist or human chorionic gonadotropin, Biochem. Biophys. Res. Commun. 76: 855.CrossRefGoogle Scholar
  5. Auclair, C., Ferland, L., Cusan, L., Kelly, P.A., Labrie, F., Azadian-Boulanger, G., and Raynaud, J.-P., 1978, Effet inhibiteur de la LHRH sur les récepteurs de la LH dans le testicule chez le rat, C. R. Acad. Sci. 286: 1305.Google Scholar
  6. Bartke, A., Williams, K.I.H., and Dalterio, S., 1977, Effect of estrogens on testicular testosterone production in vitro, Biol. Reprod. 17: 645.CrossRefGoogle Scholar
  7. Bartke, A., Hafiez, A.A., Bex, F.J., and Dalterio, S., 1978, Hormonal interaction in regulation of androgen secretion, Biol. Reprod. 18: 44.CrossRefGoogle Scholar
  8. Bélanger, A., Auclair, C., S;guin, C., Kelly, P.A., and Labrie, F., 1979, Down-regulation of testicular androgen biosynthesis and LH receptor levels by an LHRH agonist: Role of prolactin, Mo!. Ce!!. Endocrinol. 13: 47.CrossRefGoogle Scholar
  9. Bélanger, A., Caron, S., and Picard, V., 1980, Simultaneous radioimmunoassay of progestins, androgens and estrogens in rat testis, J. Steroid Biochem. 13: 123.CrossRefGoogle Scholar
  10. Bex, F.J., and Bartke, A., 1977, Testicular LH binding in the hamster: Modification by photoperiod and prolactin, Endocrinology 100: 1223.CrossRefGoogle Scholar
  11. Bohnet, H.G., and Friesen, H.G., 1976, Effect of prolactin and growth hormone on prolactin and LH receptors in the dwarf mouse, J. Reprod. Fertil. 48: 307.CrossRefGoogle Scholar
  12. Catt, K.J., Dufau, M.L., and Tsuruhara, T., 1972, Radioligand receptor assay of luteinizing hormone and chorionic gonadotropin, J, Clin. Endocrinol. Metab. 34: 123.CrossRefGoogle Scholar
  13. Catt, K.D., Bankal, A.J., Davies, T.F., and Dufau, M.L., 1979, Luteinizing hormone releasing hormone-induced regulation of gonadotropin and prolactin receptors in the rat testis, Endocrinology 104: 17.CrossRefGoogle Scholar
  14. Chen, Y.D.I., and Payne, A.J., 1977, Regulation of testicular LH receptors by homologous hormne: In vitro studies on receptor occupancy and receptor loss, Biochem. Biophys. Res. Commun. 74: 1589.CrossRefGoogle Scholar
  15. Chen, Y.D.I., Payne, A.H., and Kelch, R.P., 1976, Stimulation of Leydig cell function in the hypophysectomized immature rat, Proc. Soc. Exp. Biol. Med. 153: 473.Google Scholar
  16. Costlow, M.E., and McGuire, W.L., 1977, Autoradiographic localization of binding of /251-labeled prolactin to rat tissues in vitro, J. Endocrinol. 75: 221.CrossRefGoogle Scholar
  17. De Jong, F.H., Hey, A.H., and Van der Molen, H.J., 1973, Effect of gonadotropins on the secretion of estradiol-17ß and testosterone by rat testis, J. Endocrinol. 57: 277.CrossRefGoogle Scholar
  18. Desjardins, C., Zeleznik, A.J., and Midgley, A.R., 1975, Binding of radioiodinated gonadotropins to testes after induction of testicular dysfunction, Endocrinology 96: 145A.Google Scholar
  19. ElSafoury, S., and Bartke, A., 1974, Effects of follicle-stimulating hormone and luteinizing hormone on plasma testosterone levels in hypophysectomized and in intact immature adult male rats, J. Endocrinol. 61: 193.CrossRefGoogle Scholar
  20. Gavin, P.A., Roth, J., Neville, D.M., De Meyts, P., and Buell, D.M., 1974, Insulin-dependent regulation of insulin receptor concentrations: A direct demonstration in cell culture, Proc. Natl. Acad. Sci. U.S.A. 71: 84.CrossRefGoogle Scholar
  21. Hauger, R.L., Chen, Y.D.I., Kelch, R.P., and Payne, A.H., 1977, Pituitary regulation of Leydig cell function in the adult male rat, J. Endocrino. 74: 57.CrossRefGoogle Scholar
  22. Hinkle, P.M., and Tashjian, H.H., 1976, Thyrotropin-releasing hormone regulates the number of its own receptors in the GH3 strain of pituitary cells in culture, Biochemistry 14: 3845.CrossRefGoogle Scholar
  23. Hsueh, A.J.W., and Erickson, G.F., 1979, Extrapituitary inhibition of testicular function by luteinizing hormone-releasing hormone, Nature (London) 281: 66.CrossRefGoogle Scholar
  24. Hsueh, A.J.W., and Dufau, M., and Catt, K.J., 1976, Regulation of luteinizing hormone receptors in testicular interstitial cells by gonadotropin, Biochem. Biophys. Res. Commun. 72: 1145.CrossRefGoogle Scholar
  25. Hsueh, A.J.W., Dufau, M.L., and Catt, K.J., 1977, Gonadotropin-induced regulation of luteinizing hormone receptors and desensitization of testicular 3’,5’-cyclic AMP and testosterone responses, Proc. Natl. Acad. Sci. U.S.A. 74: 592.CrossRefGoogle Scholar
  26. Kahn, C.R., 1976, Membrane receptors for hormones and neurotransmitter, J. Cell Biol. 70: 261.CrossRefGoogle Scholar
  27. Kalla, N.R., Nisula, B.C., Ménard, R.H., and Loriaux, D., 1977, Estrogen modulation of Leydig cell function, Endocrinology 100: 82A.Google Scholar
  28. Kelly, P.A., De Léan, A., Auclair, C., Cusan, L., and Labrie, F., 1979, Regulation of peptide hormone receptors, in: Clinical Endocrinology: A Pathological Approach ( G. Tolis, F. Labrie, J.B. Martin, and F. Naftolin, eds.), pp. 183–197, Raven Press, New York.Google Scholar
  29. Kramer, C.Y., 1956, Extension of multiple range test to group means with unequal numbers of replications, Biometrics 12: 307.CrossRefGoogle Scholar
  30. Labrie, F., Auclair, C., Cusan, L., Kelly, P.A., Pelletier, G., and Ferland, L., 1978, Inhibitory effects of LHRH and its agonists on testicular gonadotropin receptors and spermatogenesis in the rat, Int. J. Andrology, Suppl. 2: 303.Google Scholar
  31. Labrie, F., Bélanger, A., Pelletier, G., Séguin, C., Cusan, L., Kelly, P.A., Lemay, A., Auclair, C., and Raynaud, J.P., 1980, LHRH agonists: Inhibition of testicular functions and possible clinical applications, in: Clinics in Andrology, Vol. 5, Regulation of Male Fertility ( G.R. Cunningham, W.B. Schill, and E.S.E. Hafez, eds.), pp. 64–75, Martinus Nijhoff, The Hague, The Netherlands.Google Scholar
  32. Lesniak, M.A., and Roth, J., 1976, Regulation of receptor concentration by homologous hormone: Effects of human growth hormone on its receptor in 1M-9 lymphocytes, J. Biol. Chem. 251: 3720.Google Scholar
  33. Mendelson, C., Dufau, M.L., and Catt, K.J., 1975, Gonadotropin binding and stimulation of cyclic adenosine 3’,5’-monophosphate and testosterone production in isolated Leydig cells, J. Biol. Chem. 250: 8818.Google Scholar
  34. Mukherjee, C., Caron, M.G., and Lefkowitz, R.L., 1975, Catecholamine-induced subsensitivity of adenylate cyclase associated with a loss of beta-adrenergic receptor binding sites, Proc. Natl. Acad. Sci. U.S.A. 72: 1945.CrossRefGoogle Scholar
  35. Odell, W.D., and Swerdloff, R.S., 1975, The role of testicular sensitivity to gonadotropins in sexual maturation of the male rat, J. Steroid Biochem. 6: 853.CrossRefGoogle Scholar
  36. Rivier, C., Rivier, J., and Vale, W., 1979, Chronic effects of ID-Trp’’,Pros NET]luteinizing hormone-releasing factor in reproductive processes in the male rat, Endocrinology 105: 1191.CrossRefGoogle Scholar
  37. Rodbard, D., and Lewald, J.E., 1970, Computer analysis of radioligand assay and radioimmunoassay data, in: Karolinska Symposia on Research Methods in Reproductive Endocrinology, No. 2 ( E. Diczfalusy, ed.), pp. 79–103, Bogtrykkeriet, Copenhagen.Google Scholar
  38. Samuels, L.T., Short, J.G., and Huseby, 1964, The effect of diethylstilbestrol on testicular 17a-hydroxylase and 17-desmolase activities in BALB/C mice, J. Endocrinol. 63: 411.Google Scholar
  39. Schwarzstein, L., 1976, Diagnostic and therapeutic use of LHRH in the infertile man, in: Hypothalamus and Endocrine Functions ( F. Labrie, J. Meites, and G. Pelletier, eds.), pp. 73–91, Plenum Press, New York.Google Scholar
  40. Sharpe, M., 1976, Relationships between testosterone, fluid content and luteinizing hormone receptors in rat testis, Nature (London) 264: 644.CrossRefGoogle Scholar
  41. Tcholakian, R.K., Chowdhury, M., and Steinberger, E., 1974, Time action of estradiol-l70 on luteinizing hormone and testosterone, J. Endocrinol. 63: 411.CrossRefGoogle Scholar
  42. Thanki, K.H., and Sternberger, A., 1976, 1251-LH binding to rat testes at various ages and post-hypophysectomy, Endocr. Res. Commun. 3: 49.Google Scholar
  43. Zeleznik, A.J., Midgley, A.R., and Reichart, L.E., 1974, Granulosa cell maturation in theGoogle Scholar
  44. rat: Increased binding of human chorionic gonadotropin following treatment with follicle-stimulating hormone, J. Biol. Chem. 250: 8818.Google Scholar
  45. Zipf, W.P., Payne, A.A., and Kelch, R.P., 1978, Prolactin, growth hormone and luteinizing hormone in the maintenance of testicular luteinizing hormone receptors, Endocrinology 103: 595.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • P. A. Kelly
    • 1
  • F. Labrie
    • 1
  • C. Auclair
    • 1
  • C. Séguin
    • 1
  • A. Bélanger
    • 1
  • M. R. Sairam
    • 2
  1. 1.Department of Molecular EndocrinologyLe Centre Hospitalier de l’Université LavalQuebecCanada
  2. 2.Institut de Recherches CliniquesMontrealCanada

Personalised recommendations