Control of Spermatogonial Multiplication

  • M. T. Hochereau-de-Reviers
Part of the Biochemical Endocrinology book series (BIOEND)


In the seminiferous tubules of the testis of adult mammals, spermatogonia are enclosed in the basal compartment of the seminiferous epithelium, with the nuclei and the basal cytoplasm of Sertoli cells, close to the basal membrane (Dym and Fawcett, 1970).


Follicle Stimulate Hormone Sertoli Cell Primary Spermatocyte Cyproterone Acetate Seminiferous Epithelium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ahmad, N., Haltmayer, G.C., and Eik-Nes, K., 1975, Maintenance of spermatogenesis with testosterone or dihydrotestosterone in hypophysectomized rats, J. Reprod. Fertil. 44: 103–107.CrossRefGoogle Scholar
  2. Aizawa, S., and Nishimune, Y., 1979, In vitro differentiation of type A spermatogonia in mouse cryptorchid testis. J. Reprod. Fertil. 56: 99–104.CrossRefGoogle Scholar
  3. Attal, J., and Courot, M., 1963. Développement testiculaire et établissement de la spermatogenèse chez le taureau, Ann. Biol. Anim. Biochim. Biophys. 3: 219–242.CrossRefGoogle Scholar
  4. Aumüller, G., Schenk, B., and Neumann, F., 1975, Fine structure of monkey (Macaca mulatta) Sertoli cells after treatment with cyproterone, Andrologia 7: 317–328.CrossRefGoogle Scholar
  5. Baker, H.W.G., Bremner, W.J., Burger, H.G., De Kretser, D.M., Dulmanis, A., Eddie, L.W., Hudson, B., Keogh, E.J., Lee, V.W.K., Rennie, G.C., 1976, Testicular control of FSH secretion, Recent Prog. Horm. Res. 32: 429–469.Google Scholar
  6. Baker, H.W.G., Burger, H.G., De Kretser, D.M., Eddie, L.W., Higginson, R.E., Hudson, B., Lee, V.W.K., 1978, Studies on purification of inhibin from ovine testicular secretions using an in vitro bioassay, Int. J. Andr. Suppl. 2: 115–124.CrossRefGoogle Scholar
  7. Blanc, M.R., and Dacheux, J.L., 1976, Existence of inhibin activity in the ram rete testis fluid: Effect of physiological doses on plasma LH and FSH, RCS Med. Sci. 4: 460.Google Scholar
  8. Blanc, M.R., Cahoreau, C., Courot, M., Dacheux, J.L., Hochereau-de-Reviers, M.T., and Pisselet, C., 1978, Plasma follicle stimulating hormone (FSH) and luteinizing hormone (LH) suppression in the cryptorchid ram by a nonsteroid factor (inhibin) from ram rete testis fluid, Int. J. Androl. Suppl. 2: 139–146.CrossRefGoogle Scholar
  9. Boccabella, A.V., 1963, Reinitiation and restoration of spermatogenesis with testosterone propionate and other hormones after a long term post hypophysectomy regression period, Endocrinology 72: 787–798.CrossRefGoogle Scholar
  10. Bowler, K., 1972, The Effect of repeated applications of heat on spermatogenesis in the rat: A histological study, J. Reprod. Fertil. 28: 325–334.CrossRefGoogle Scholar
  11. Bullough, W.S., 1962, The control of mitotic activity in the adult mammalian tissue, Biol. Rev. 37: 307.CrossRefGoogle Scholar
  12. Cahoreau, C., Blanc, M.R., Dacheux, J.L., Pisselet, C., Courot, M., 1978, Inhibin activity in ram rete testis fluid: Depression of plasma FSH and LH in the castrated and cryptorchid ram, J. Reprod. Fert. Suppl. 26: 97–116.Google Scholar
  13. Chasalow, F., Marr, H., Haour, F., and Saez, J.M., 1979, Testicular steroidogenesis after human chorionic gonadotropin desensitization in rats, J. Biol. Chem. 254: 5613–5617.Google Scholar
  14. Chemes, H.E., Podesta, E., and Rivarola, M.A., 1976, Action of testosterone, dihydrotestosterone and 5a-androstan-3a-l7ß diol on spermatogenesis of immature rat testis, Endocrinology 72: 787–798.Google Scholar
  15. Chiu, M.L., and Irvin, J.L., 1978, Basic chromosomal proteins in evaluation of the arrest and restoration of spermatogenesis, Biol. Reprod. 19: 984–993.CrossRefGoogle Scholar
  16. Chowdhury, A.K., 1979, Dependence of testicular germ cell on hormones: A quantitative study in hypophysectomized testosterone treated rats, J. Endocrinol. 82: 331–340.CrossRefGoogle Scholar
  17. Chowdhury, A.K., and Steinberger, E., 1964, A quantitative study of the effect of heat on germinal epithelium of rat testis, Am. J. Anat. 115: 509–524.CrossRefGoogle Scholar
  18. Clermont, Y., and Antar, M., 1973, Duration of the cycle of the seminiferous epithelium and the spermatogonial renewal in the monkey Macaca arctoides, Am. J. Anat. 136: 153–166.CrossRefGoogle Scholar
  19. Clermont, Y., and Bustos-Obregon, E., 1968, Reexamination of spermatogonial renewal in the rat by means of seminiferous tubules mounted “in toto,” Am. J. Anat. 122: 237–248.CrossRefGoogle Scholar
  20. Clermont, Y., and Harvey, S.C., 1967, Effects of hormones on spermatogenesis in the rat, in: Endocrinology of the Testis, Ciba Foundation Colloquium No. 16 (G.E.W.Wolstenholme and M. O’Connor, eds.), pp. 173–189, Churchill, London.Google Scholar
  21. Clermont, Y., and Mauger, A., 1974, Existence of a spermatogonial chalone in the rattestis, Cell Tissue Kinet. 7: 165–172.Google Scholar
  22. Clermont, Y., and Mauger, A., 1976, Effect of a spermatogonial chalone in the growing rat testis. Cell Tissue Kinet. 9: 99–104Google Scholar
  23. Clermont, Y., and Morgentaler, H., 1955, Quantitative study of spermatogenesis in the hypophysectomized rat, Endocrinology 57: 369–382.CrossRefGoogle Scholar
  24. Courot, M., 1967, Endocrine control of the supporting and germ cells of the impuberal testis, J. Reprod. Fertil. Suppl. 2: 89–101.Google Scholar
  25. Courot, M., Hochereau-de-Reviers, M.T., and Ortavant, R., 1970, Spermatogenesis, in:The Testis, Vol. I ( A.D. Johnson, W.R. Gomes, and N.L. Vandemark, eds.), pp. 339–432, Academic Press, New York.Google Scholar
  26. Courot, M., Ortavant, R., and de Reviers, M.M., 1971, Variations du contrôle gonadotrope du testicule selon l’âge des animaux, Exp. Anim. 4: 201–211.Google Scholar
  27. Courot, M., Hochereau-de-Reviers, M.T., Monet-Kuntz, C., Locatelli, A., Pisselet, C., Blanc, M.R., and Dacheux, J.L., 1979, Endocrinology of spermatogenesis in the hypophysectomized ram, J. Reprod. Fertil. Suppl. 26: 165–173.Google Scholar
  28. Cunningham, G.R., and Huckins, C., 1979a, Failure to identify a spermatogonial chalone in adult irradiated testes, Cell Tissue Kinet. 12: 81–89.Google Scholar
  29. Cunningham, G.R., and Huckins, C., 1979b, Persistence of complete spermatogenesis in the presence of low intratesticular concentrations of testosterone, Endocrinology 105: 177–186.CrossRefGoogle Scholar
  30. Debeljuk, L., and Mancini, R.E., 1971, Pituitary gonadotrophin activity in male rats with damage of the germinal epithelium induced by busulfan, J. Reprod. Fertil. 26: 247–250.CrossRefGoogle Scholar
  31. de Jong, F.H., Welschen, R., Hermans, W.P., Smith, S.D., and Van der Molen, H.J., 1978, Effects of testicular and ovarian inhibin-like activity, using in vitro and in vivo systems, Int. J. Androl. Suppl. 2: 125–138.CrossRefGoogle Scholar
  32. de Kretser, D.M., Burger, H.G., and Hudson, B., 1973, The relationship between germinal cells and serum FSH levels in males with infertility, J. Clin. Endocrinol. Metab. 38: 787–793.CrossRefGoogle Scholar
  33. de Reviers, M. Hochereau-de-Reviers, M.T., Blanc, M.R., Brillard, J.P., Courot, M., and Pelletier, J., 1980, Control of Sertoli and germ cells populations in cock and sheep testis, Reprod. Nutr. Develop. 20: 241–249.CrossRefGoogle Scholar
  34. De Rooij, D.G., 1973, Spermatogonial stem cell renewal in the mouse. I. Normal situation, Cell Tissue Kinet. 6: 281–287.Google Scholar
  35. Dubois, M.P., de Reviers, M.M., and Courot, M., 1971, Activité gonadotrope de l’éminence médiane après hypophysectomie chez le rat: Etude en immunofluorescence, Exp. Anim. 4: 213–226.Google Scholar
  36. Dym, M., and Clermont, Y., 1970, Role of spermatogonia in the repair of the seminiferous epithelium following X irradiation of the rat testis, Am. J. Anat. 128: 265–281.CrossRefGoogle Scholar
  37. Dym, M., and Fawcett, D.W., 1970, The blood testis barrier in the rat and the physiological compartmentation of the seminiferous epithelium, Biol. Reprod. 3: 308–326.Google Scholar
  38. Eshkol, A., and Lunenfeld, B., 1971–1972, Biological effects of antibodies to gonadotropins, Gynecol. Invest. 2: 23–56.Google Scholar
  39. Fabbrini, A., Spera, G., Santiemma, V., and Fraioli, F., 1974, Autoradiographic and spectrometric study of human gonadotrophine; Localization in target organs, in: Male Fertility and Sterility ( R.E. Mancini and L. Martini, eds.), pp. 389–404, Academic Press, New York.Google Scholar
  40. Flickinger, C.J., and Loving, L.K., 1976, Fine structure of the testis and epididymis of rats treated with cyproterone acetate, Am. J. Anat. 146: 359–384.CrossRefGoogle Scholar
  41. Franchimont, P., Millet, D., Vendrely, E., Letame, J., Legros, J.J., and Netter, A., 1972, Relationship between spermatogenesis and serum gonadotrophin levels in azoospermia and oligospermia, J. Clin. Endocrinol. Metab. 34: 1003–1008.CrossRefGoogle Scholar
  42. Franchimont, P., Chad, S., Hazee-Hagelstein, M.T., Debruche, M.L., and Duraiswami, S., 1977, Evidence for existence of inhibin, in: The Testis in Normal and Infertile Men ( P. Troen and H.R. Nankin, eds.), pp. 253–270, Raven Press, New York.Google Scholar
  43. Franchimont, P., Demoulin, A., Hazee-Hagelstein, M.T., Verstraelen-Proyard, J., and Bourguignon, J.P., 1979, Control of gonadotrophin secretion by inhibin, Acta Endocrinol. Suppl. 225: 469.Google Scholar
  44. Frankenhuis, M.T., and Wensing, C.J.G., 1979, Induction of spermatogenesis in the naturally cryptorchid pig. Fertil. Steril. 31: 428–433.Google Scholar
  45. Gomes, W.R., Hall, R.W., Jain, S.K., and Boots, L.R., 1973, Serum gonadotropin and testosterone levels during loss and recovery of spermatogenesis in rats, Endocrinology 93: 800–809.CrossRefGoogle Scholar
  46. Harris, M.E., Bartke, A., Weisz, J., and Watson, D., 1977, Effects of testosterone and dihydrotestosterone on spermatogenesis, rete-testis fluid and peripheral androgen levels in hypophysectomized rats. Fertil. Steril. 28: 1113–1117.Google Scholar
  47. Heinert, G., and Taubert, H.D., 1973, Effect of cyproterone and cyproterone acetate on testicular function in the rat: A karyometric study, Endokrinologie 61: 168–178.Google Scholar
  48. Hochereau, M.T., 1967, Synthèse de l’ADN au cours des multiplications et du renouvellement des spermatogonies chez le taureau, Arch. Anat. Microsc. Morphol. Exp. 56(Suppl. 3–4):85–96.Google Scholar
  49. Hochereau-de-Reviers, M.T., 1970, Etude des divisions spermatogoniales et du renouvellement de la spermatogonie souche chez le taureau, D. Sci. thesis, Paris, C.N.R.S. A03976.Google Scholar
  50. Hochereau-de-Reviers, M.T., 1971, Etude cinétique des spermatogonies chez les mammifères—revue, in: La Cinétique de Prolifération cellulaire, Vol. 18, INSERM Symposia Series Seminar, pp. 189–216.Google Scholar
  51. Hochereau-de-Reviers, M.T., 1976, Variation in the stock of testicular stem cells and in the yield of spermatogonial divisions in ram and bull testes, Andrologia 8: 137–146.CrossRefGoogle Scholar
  52. Hochereau-de-Reviers, M.T., and Courot, M., 1978, Sertoli cells and development of seminiferous epithelium, Ann. Biol. Anim. Biochim. Biophys. 18: 573–583.CrossRefGoogle Scholar
  53. Hochereau-de-Reviers, M.T., and Lincoln, G.A., 1978, Seasonal variation in the histology of the testis of the red deer, Cervus elaphus, J. Reprod. Fertil. 54: 209–213.CrossRefGoogle Scholar
  54. Hochereau-de-Reviers, M.T., Loir, M., and Pelletier, J., 1976a, Seasonal variations in the response of the testis and LH levels to hemicastration of adult rams, J. Reprod. Fertil. 46: 203–209.CrossRefGoogle Scholar
  55. Hachereau-de Reviers, M.T., Ortavant, R., and Courot, M., 1976b, Type A spermatogonia in the ram, in: Progress in Reproductive Biology, Vol. 1, Sperm Action ( Hachereau-de Reviers, M.T., Ortavant, R., and Courot, M., ed.), pp. 13–19, S. Karger Basel.Google Scholar
  56. Hochereau-de-Reviers, M.T., Blanc, M.R., Cahoreau, C., Courot, M., Dacheux, J.L., and Pisselet, C., 1979, Histological testicular parameters in bilateral cryptorchid adult rams. Ann Biol. Anim. Biochim. Biophys. 19: 1141–1146.CrossRefGoogle Scholar
  57. Hochereau-de-Reviers, M.T., Blanc, M.R., Courot, M., Garnier, D.H., Pelletier, J., and Poirier, J.C., 1980, Hormonal profiles and testicular parameters in the lamb, in: Testicular Development, Structure and Function ( E. Steinberger and A. Steinberger, eds.), pp. 237–247, Raven Press, New York.Google Scholar
  58. Huckins, C., 1971, The spermatogonial stem cell population in adult rats. 1. Their morphology, proliferation and maturation, Anat. Rec. 169: 533–558.CrossRefGoogle Scholar
  59. Huckins, C., 1978, The morphology and kinetics of spermatogonial degeneration in normal adult rats: An analysis using a simplified classification of the germinal epithelium, Anat. Rec. 190: 905–926.CrossRefGoogle Scholar
  60. Irons, M.J., and Clermont, Y., 1979, Spermatogonial chalone. Effect on the phases of the cell cycle of type A spermatogonia in the rat, Cell Tissue Kinet. 12: 425–433.Google Scholar
  61. Kalra, S.P., and Prasad, M.R.N., 1967, Effect of FSH and testosterone propionate on spermatogenesis in immature rats treated with clomiphene, Endocrinology 81: 965–975.CrossRefGoogle Scholar
  62. Krueger, P.M., Hogden, G.D., and Sherins, R.J., 1974, New evidence for the role of the Sertoli cell and spermatogonia in feed-back control of FSH secretion in male rats, Endocrinology 95: 355–362.CrossRefGoogle Scholar
  63. Land, R.B., and Hochereau-de-Reviers, M.T., 1980, Variations in testis growth and histology in 6 month old rams, normal or hemicastrated, according to season of birth, J. Reprod. Fertil., to be published.Google Scholar
  64. Mancini, R.E., 1971, Effect of gonadotropin preparations and of urinary FSH and LH on human spermatogenesis, in: The Regulation pl. Mammalian Reproduction ( S.J. Segal, R. Crozier, P.A. Corfman, and P.G. Condliffe, eds.), pp. 151–162, Charles C. Thomas, Springfield, Illinois.Google Scholar
  65. Matsuyama, S., Ogasa, Q., and Yokoki, H., 1971, Quantitative study of spermatogenesis in androgen treated hypophysectomized rats, Natl. Inst. Anim. Health Q. (Tokyo) 11: 46–52.Google Scholar
  66. Means, A.R., 1977, Mechanism of action of follicle stimulating hormone (FSH), in: The Testis, Vol. IV ( A.D. Johnson and W.R. Gomes, eds.), pp. 168–188, Academic Press, New York.Google Scholar
  67. Mills, N.C., and Means, A.R., 1972, Sorbitol dehydrogenase of rat testis: Change of activity during development, after hypophysectomy and following gonadotrophic hormone administration, Endocrinology 91: 147–156.CrossRefGoogle Scholar
  68. Monastirky, R., Laurence, K.A., and Tovar, E., 1971, The effects of gonadotropin immunization of prepubertal rabbits on gonadal development, Fertil. Steril. 22: 318–324.Google Scholar
  69. Monesi, V., 1962, Autoradiographic study of DNA synthesis and the cell cycle in spermatogonia and spermatocytes of mouse testis using tritiated thymidine, J. Cell Biol. 14: 1–18.CrossRefGoogle Scholar
  70. Monet-Kuntz, C., Terqui, M., Locatelli, A., Hochereau-de-Reviers, M.T., and Courot, M., 1976, Effets de la supplémentation en testosterone sur la spermatogenèse de béliers hypophysectomisés, C. R. Acad. Sci. Ser. D 283: 1763–1766.Google Scholar
  71. Murty, G.S.R.C., Rani, C.S., Mougdal, N.R., 1979, Effects of passive immunization with specific antiserum to FSH on the spermatogenic process and fertility of adult male Bonnet monkeys (Maccaca radiata), J. Reprod. Fert. 26: 147–163.Google Scholar
  72. Nagy, F., 1972, Cell division kinetics and DNA synthesis in the immature Sertoli cells of the rat testis, J. Reprod. Fertil. 28: 389–395.CrossRefGoogle Scholar
  73. Neumann, F., and Von Berswordt-Wallrabe, R., 1966, Effects of the androgen antagonist cyproterone acetate on the testicular structure, spermatogenesis and accessory sexual glands of testosterone treated adult hypophysectomized rats, J. Endocrinol. 35: 363–371.CrossRefGoogle Scholar
  74. Oakberg, E.F., 1971, Spermatogonial stem cell renewal in the mouse, Anat. Rec. 169: 515–532.CrossRefGoogle Scholar
  75. Ortavant, R., 1958, Le cycle spermatogénétique chez le bélier, D. Sci. thesis, Paris, 127 pp, CNRS A-3118.Google Scholar
  76. Ortavant, R., Courot, M., and Hochereau-de-Reviers, M.T., 1972, Gonadotrophic control of tritiated thymidine incorporation in the germinal cells of the rat testis, J. Reprod. Fertil. 31: 451–453.CrossRefGoogle Scholar
  77. Orth, J., and Christensen, A.K., 1978, Autoradiographic localization of specifically bound ‘251 labelled follicle stimulating hormone on spermatogonia of the rat testis, Endocrinology 103: 1944–1951.CrossRefGoogle Scholar
  78. Picaper, G., Hochereau-de-Reviers, M.T., Martinat, N., and Dubois, M.P., 1979, Inhibition of post hemicastration rise in Sertoli cell stocks in prepubertal rats by proteinaceous pregnant sow corpora lutea and pubertal rat testis extracts, IRCS Med. Sci. 7: 132.Google Scholar
  79. Raj, H.G.M., Dym, M., Chemes, H.E., Kotite, N.J., Nayfeh, S.N., and French, F.S., 1978a, Effects of passive immunity to FSH on male reproduction in the immature and adult rat, Proc. 3rd Ann. Meet. Am. Soc. Androl. (abstract).Google Scholar
  80. Raj, H.G.M., Dym, M. Sairam, M.R., and Chendy, R., 1978b, Effects of selective depletion of luteinizing hormone and testosterone on the pituitary gonadal axis, lilt. J. Androl. Suppl. 2: 184–189.CrossRefGoogle Scholar
  81. Regaud, C., 1901, Etude sur la structure des tubes séminifères et sur la spermatogenèse chez les mammifères, Arch. Anat. Microsc. 101: 231–380.Google Scholar
  82. Rivarola, M.A. Podesta, F.J., Chemes, H.E., and Calandra, R.S., 1977, Hormonal influences on the initiation of spermatogenesis, in: Endocrinology, Vol. I ( V.H.T. James, ed.), pp. 307–313, Excerpta Medica, Amsterdam.Google Scholar
  83. Roosen-Runge, E.C., and Giesel, L.O., 1950, Quantitative studies on spermatogenesis in the albino rat, Am. J. Anat. 87: 1–30.CrossRefGoogle Scholar
  84. Setchell, B.P., Davies, R.V., and Main, S.J., 1977, Inhibin, in: The Testis, Vol. IV (A.D.Johnson and W.R. Gomes, eds.), pp. 189–238, Academic Press, New York.Google Scholar
  85. Steinberger, A., 1979, Inhibin production by Sertoli cells in culture, J. Reprod. Jertil.Suppl. 26: 31–45.Google Scholar
  86. Steinberger, A., and Steinberger, E., 1966,In vitro culture of rat testicular cells, Exp. Cell Res. 44:443–452.CrossRefGoogle Scholar
  87. Steinberger, A., and Steinberger, E., 1971, Replication pattern of Sertoli cells in maturing rat testis in vivo and in organ cultures, Biol. Reprod. 4: 84–87.Google Scholar
  88. Steinberger, E., 1971, Hormonal control of mammalian spermatogenesis, Physiol. Rev. 51:1–22.Google Scholar
  89. Steinberger, E., and Duckett, G.E., 1967, Hormonal control of spermatogenesis, J. Reprod. Fertil. Suppl.2:75–87.Google Scholar
  90. Steinberger, E., Steinberger, A., and Perloff, H., 1963, In vitro growth of testicular fragments from rats of various ages,Anat. Rec.145:288.Google Scholar
  91. Thumann, A., and Bustos-Obregon, E., 1978, An “in vitro” system for the study of rat spermatogonial proliferation control, Andrologie 10: 22–25.Google Scholar
  92. Turner, P.C., and Johnson, A.D., 1971, The effect of anti FSH serum on the reproductive organs of the male rat, Int. J. Fertil. 16: 169–176.Google Scholar
  93. Van Keulen, C.J.G., and de Rooij, D.G., 1974, The recovery from various gradations of cell loss in the mouse seminiferous epithelium and its implication for the spermatogonial stem cell renewal theory. Cell Tissue Kinet. 7: 549–558.Google Scholar
  94. Vernon, R.G., Go, V.L.W., and Fritz, I.B., 1975, Hormonal requirements of the different cycles of the seminiferous epithelium during reinitation of spermatogenesis in long term hypophysectomized rats, J. Reprod. Fertil. 42: 77–94.CrossRefGoogle Scholar
  95. Viguier-Martinez, M.C., and Hochereau-de-Reviers, M.T., 1977, Comparative action of cyproterone and cyproterone acetate on pituitary and plasma gonadotropin levels, the male genital tract and spermatogenesis in the growing rat, Ann. Biol. Anim. Biochim. Biophys. 17: 1069–1076.CrossRefGoogle Scholar
  96. Waites, G.W., and Ortavant, R., 1968, Effets précoces d’une bréve élévation de la température testiculaire sur la spermatogenèse du bélier, Ann. Biol. Anim. Biochim. Biophys. 8: 323–331.CrossRefGoogle Scholar
  97. Wettemann, R.P., and Desjardins, C., 1979, Testicular function in boars exposed to elevated temperature, Biol. Reprod. 20: 235–241.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • M. T. Hochereau-de-Reviers
    • 1
  1. 1.Station de Physiologie de la ReproductionINRANouzillyFrance

Personalised recommendations