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Hormonal Control of Steroid Receptors in Human Endometrium during the Menstrual Cycle

  • Paul Robel
  • Rodrigue Mortel
  • Etienne-Emile Baulieu
Part of the Biochemical Endocrinology book series (BIOEND)

Abstract

In the past two decades, the development of saturation-analysis methods has permitted specific and accurate measurements of circulating sex-steroid hormones, and their cyclic changes during the menstrual cycle are well documented. For the past ten years, a large body of evidence has been accumulated indicating that hormones interact with a receptor system before triggering cellular responses. Hormone receptors were first identified and physicochemically studied for steroids, particularly estradiol in the rat uterus. Once reliable measurements of hormone receptors became available in animal models, correlations were attempted between receptor concentrations and circulating levels of various hormones. It was discovered that the concentration of receptor molecules is not fixed, but varies with the physiological state of the animals. In particular, variations of the concentrations and subcellular distributions of receptors were observed during the estrous cycle and mimicked by injecting estradiol and progesterone into hormone-deprived animals (Bau-lieu et al., 1975).

Keywords

Progesterone Receptor Receptor Site Human Endometrium Estradiol Receptor Human Uterus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Anderson, J., Clark, J.H., and Peck, E.J., 1972, Oestrogen and nuclear binding sites: Determination of specific sites by 3H-oestradiol exchanges, Biochem. J. 126: 561–567.Google Scholar
  2. Batra, S., Grundsell, H., and Sjöberg, N.O., 1977, Estradiol-1713 and progesterone concentrations in human endometrium during the menstrual cycle, Contraception 16: 217–224.CrossRefGoogle Scholar
  3. Baulieu, E.-E., Atger, M., Best-Belpomme, M., Corvol, P., Courvalin, J.C., Mester, J., Milgrom, E., Robel, P., Rochefort, H., and De Catalogne, D., 1975, Steroid hormones receptors, Vitam. Horm. 33: 649–736.CrossRefGoogle Scholar
  4. Bayard, F., Louvet, J.P., Monrozies, M., Boulard, A., and Pontonnier, G., 1975, Endometrial progesterone concentrations during the menstrual cycle, J. Clin. Endocrinol. Metab. 41: 412–414.CrossRefGoogle Scholar
  5. Bayard, F., Damilano, S., Robel, P., and Baulieu, E.-E., 1978, Cytoplasmic and nuclear estradiol and progesterone receptors in human endometrium, J. Clin. Endocrinol. Metab. 46: 635–648.CrossRefGoogle Scholar
  6. Brenner, R.M., Resko, J.A., and West, N.B., 1974, Cyclic changes in oviductal morphology and residual cytoplasmic estradiol binding capacity induced by sequential estradiolprogesterone treatment of spayed rhesus monkeys, Endocrinology 95: 1094–1104.CrossRefGoogle Scholar
  7. Briggs, M.H., 1975, Comparative steroid binding to the human uterine progesterone-receptor, Carr. Med. Res. Opinion 3: 95–98.CrossRefGoogle Scholar
  8. Brush, M.G., Taylor, R.W., and King, R.J.B., 1967, The uptake of (6,7–3H) oestradiol by the normal human female reproductive tract, J. Endocrinol. 39: 599–607.CrossRefGoogle Scholar
  9. Brush, M.G., King, R.J.B., and Taylor, R.W., 1978, Endometrial Cancer,Baillière Tindall,London.Google Scholar
  10. Coffer, Al.,. Milton, P.J.D., Pryse-Davies, J., and King, R.J.B., 1977, Purification of oestradiol receptor from human uterus by affinity chromatography, Mol. Cell. Endocrine!. 6: 231–246.CrossRefGoogle Scholar
  11. Collins, J.A., and Jewkes, D.M., 1974, Progesterone metabolism by proliferative and secretory human endometrium, Am. J. Obstet. Gynecol. 118: 179–185.Google Scholar
  12. Crocker, S.G., Milton, P.J.D., and King, R.J.B., 1974, Uptake of 6,7 3H-estradiol 17$ by normal and abnormal human endometrium, J. Endocrinol. 62: 145–152.CrossRefGoogle Scholar
  13. Davis, M.E., Wiener, M., Jacobson, H.I., and Jensen, E.V., 1963, Estradiol metabolism in pregnant and non-pregnant women, Am. J. Obstet. Gynecol. 87: 979–990.Google Scholar
  14. Evans, L.H., and Hähnel, R., 1971, Oestrogen receptors in human uterine tissue, J.Endocrine!. 50: 209–229.CrossRefGoogle Scholar
  15. Evans, L.H., Martin, J.D., and Hähnel, R., 1974, Oestrogen receptor concentration in normal and pathological human uterine tissues, J. Clin. Endocrinol. Metab. 38: 23–32.CrossRefGoogle Scholar
  16. Feil, P.D., Glasser, S.R., Toft, D.O., and O’Malley, B.W., 1972, Progesterone binding in the mouse and rat uterus, Endocrinology 91: 738–746.CrossRefGoogle Scholar
  17. Guerrero, R., Landgren, B.M., Montiel, R., Cekan, Z., and Diczfalusy, E., 1975, Unconjugated steroids in the human endometrium, Contraception 11: 169–177.CrossRefGoogle Scholar
  18. Gurpide, E., Gusberg, S.B., and Tseng, L., 1976, Estradiol binding and metabolism in human endometrial hyperplasia and adenocarcinoma, J. Steroid Biochem. 7: 891–896.CrossRefGoogle Scholar
  19. Gurpide, E., Tseng, L., and Gusberg, S.B., 1977, Estrogen metabolism in normal and neoplastic endometrium, Am. J. Obstet. Gynecol. 129: 809–816.Google Scholar
  20. Gusberg, S.B., 1976, The individual at high risk for endometrial carcinoma, Am. J. Obstet. Gynecol. 126: 535–542.Google Scholar
  21. Hähnel, R., 1971, Properties of the estrogen receptor in the soluble fraction of human uterus, Steroids 17: 105–132.CrossRefGoogle Scholar
  22. Hähnel, R., Twaddle, E., and Bundle, L., 1974, Influence of enzymes on the estrogen receptors of human uterus and breast carcinoma, Steroids 24: 489–506.CrossRefGoogle Scholar
  23. Haukkamaa, M., 1974, Binding of progesterone by rat myometrium during pregnancy and by human myometrium in late pregnancy, J. Steroid Biochem. 5: 73–79.CrossRefGoogle Scholar
  24. Haukkamaa, M., and Luukkainen, T., 1974, The cytoplasmic progesterone receptor of human endometrium during the menstrual cycle, J. Steroid Biochem. 5: 447–452.CrossRefGoogle Scholar
  25. Heyns, W., and De Moor, P., 1971, The binding of 17ß-hydroxy-5a-androstan-3-one to the steroid binding ß globulin in human plasma, as studied by means of ammonium sulfate precipitation, Steroids 18: 709–730.CrossRefGoogle Scholar
  26. Hsueh, A.J.W., Peck, E.J., and Clark, J.H., 1975, Progesterone antagonism of the oestrogen receptor and oestrogen induced uterine growth, Nature (London) 254: 337–339.CrossRefGoogle Scholar
  27. lllingworth, D.V., Wood, G.P., Flickinger, G.L., and Mikhail, B., 1975, Progesterone receptor of the human myometrium, J. Clin. Endocrinol. Metab. 40: 1001–1008.CrossRefGoogle Scholar
  28. Jänne, O., Kontula, K., Luukkainen, T., and Vihko, R., 1975, Oestrogen induced progesterone receptor in human uterus, J. Steroid Biochem. 6: 501–509.CrossRefGoogle Scholar
  29. Jänne, O., Kontula, K., and Vihko, R., 1976, Progestin receptors in human tissues: Concentrations and binding kinetics, J. Steroid Biochem. 7: 1061–1068.CrossRefGoogle Scholar
  30. Koenders, A.J., Geurts-Moespot, H., Kho, K.H., and Benraad, T.J., 1978, Estradiol and progesterone receptor activities in stored lyophilised target tissue, J. Steroid Biochem. 9: 947–950.CrossRefGoogle Scholar
  31. Kontula, K., 1975, Progesterone-binding protein in human myometrium: Binding site correlation in relation to endogenous progesterone and estradiol-17ß levels, J. Steroid Biochem. 6: 1555–1561.CrossRefGoogle Scholar
  32. Kontula, K., Jänne, O., Luukkainen, T., and Vihko, R., 1974, Progesterone-binding protein in human myometrium: Influence of metal ions on binding, J. Clin. Endocrinol. Metab. 38: 500–503.CrossRefGoogle Scholar
  33. Kontula, K., Jänne, O., Vihko, R., De Jaer, E., De Visser, J., and Zeelen, F., 1975, Progesterone-binding proteins: In Vitro binding and biological activity of different steroidal ligands, Acta Endocrinol. 78: 574–592.Google Scholar
  34. Kreitmann, B., Derache, B., and Bayard, F., 1978, Measurement of the corticosteroid binding globulin, progesterone and progesterone “receptor” content in human endometrium, J. Clin. Endocrinol. Metab. 47: 350–353.CrossRefGoogle Scholar
  35. Krishnan, A.R., Hingorani, V., and Laumas, K.R., 1973, Binding of:’H-oestradiol with receptors in the human endometrium and myometrium, Acta Endocrinol. 74: 756–758.Google Scholar
  36. Levy, C., Mortel, R., Eychenne, B., Robel, P., and Baulieu, E.-E., 1980a, Unoccupied nuclear oestradiol-receptor sites in normal human endometrium, Biochem. J. 185: 733–738.Google Scholar
  37. Levy, C., Eychenne, B., and Robel, P., 1980b, Assay of nuclear estradiol receptor by exchange on glass fiber filters, Biochim. Biophys. Acta 630: 301–305.CrossRefGoogle Scholar
  38. Levy, C., Robel, P., Gautray, J.P., DeBrux, J., Verma, U., Descomps, B., Baulieu, E.-E., and Eychenne, B., 1980e, Estradiol and progesterone receptors in human endometrium: Normal and abnormal menstrual cycles and early pregnancy, Am. J. Obstet. Gynecol. 136: 646–651.Google Scholar
  39. Lippman, M., Huff, K., Bolman, G., and Neifeld, J.P., 1977, Interactions of R5020 with progesterone and glucocorticoid receptors in human breast cancer and peripheral blood lymphocytes in vitro, in: Progesterone Receptors in Normal and Neoplastic Tissues ( W.L. McGuire, J.Y. Raynaud, and E.-E. Baulieu, eds.), pp. 193–210, Raven Press, New York.Google Scholar
  40. Lunan, C.B., and Green, B., 1975, Oestradiol-l7ß uptake in vitro into the nuclei of endometrium from different regions of the human uterus, Acta Endocrinol. 78: 353–363.Google Scholar
  41. MacLaughlin, D.T., and Richardson, G.S., 1976, Progesterone binding by normal and abnormal human endometrium, J. Clin. Endocrinol. Metab. 42: 667–678.CrossRefGoogle Scholar
  42. Makler, A., and Eisenfeld, A.J., 1974,In vitro binding of 3H-estradiol to macromolecules from the human endometrium, J. Clin. Endocrinol. Metab. 38: 628–633.CrossRefGoogle Scholar
  43. Mercier-Bodard, C., Alfsen, A., and Baulieu, E.-E., 1970, Sex steroid binding plasma protein (SBP), Acta Endocrinol. 64 (Suppl. 147): 204–224.Google Scholar
  44. McGuire, W.L., Raynaud, J.P., and Baulieu, E.-E. (eds.), 1977, Progesterone Receptors in Normal and Neoplastic Tissues, Raven Press, New York.Google Scholar
  45. Mester, J., Martel, D., Psychoyos, A., and Baulieu, E.-E., 1974, Hormonal control of oestrogen receptor in uterus and receptivity for ovoimplantation in the rat, Nature (London) 250: 776–778.CrossRefGoogle Scholar
  46. Milgrom, E., and Baulieu, E.-E., 1970, Progesterone in uterus and plasma. 1. Binding in rat uterus 105,000 g supernatant, Endocrinology 87: 276–287.CrossRefGoogle Scholar
  47. Milgrom, E., Atger, M., Perrot, M., and Baulieu, E.-E., 1972, Progesterone in uterus and plasma. VI. Uterine progesterone receptors during the estrus cycle and implantation in the guinea pig, Endocrinology 90: 1071–1078.CrossRefGoogle Scholar
  48. Milgrom, E., Luu Thi, M., Atger, M., and Baulieu, E.-E., 1973, Mechanisms regulating the concentration and the conformation of progesterone receptor(s) in the uterus, J.Biol. Chem. 248: 6366–6374.Google Scholar
  49. Notides, A.C., Hamilton, D.E., and Rudolph, J.H., 1972, Estrogen binding proteins of the human uterus, Biochim. Biophys. Acta 271: 214–224.CrossRefGoogle Scholar
  50. Philibert, D., and Raynaud, J.P., 1974, Binding of progesterone and R 5020, a highly potent progestin, to human endometrium and myometrium, Contraception 10: 457–466.CrossRefGoogle Scholar
  51. Pollow, K., Lübbert, H., Boquoi, E., Kreuzer, G., and Pollow. B., 1975a, Characterization and comparison of receptors for l7ß-estradiol and progesterone in human proliferative endometrium and endometrial carcinoma, Endocrinology 96: 319–328.CrossRefGoogle Scholar
  52. Pollow, K., Lübbert, H., Boquoi, E., Kreuzer, G., Jeske, R., and Pollow, B., 1975b, Studies on l7ß-hydroxysteroid dehydrogenase in human endometrium and endometrial carcinoma, Acta Endocrinol. 79: 134–145.Google Scholar
  53. Pollow, K., Lübbert, H., Boquoi, E., and Pollow, B., 1975c, Progesterone metabolism in normal endometrium during the menstrual cycle and in endometrial carcinoma, J. Clin. Endocrinol. Metab. 41: 729–737.CrossRefGoogle Scholar
  54. Pollow, K., Boquoi, E., Schmidt-Gollwitzer, M., and Pollow, B., 1976, The nuclear estradiol and progesterone receptors of human endometrium and endometrial carcinoma, J. Mol. Med.,1:325–342.Google Scholar
  55. Pollow, K., Schmidt-Gollwitzer, M., Boquoi, E., and Pollow, B., 1978, Influence of estrogens and gestagens on 17ß-hydroxysteroid dehydrogenase in human endometrium and endometrial carcinoma, J. Mol. Med. 3: 81–89.Google Scholar
  56. Powell, B., Garola, R.E., Chamness, G.C., and McGuire, W.L., 1979, Measurement of progesterone receptor in human breast cancer biopsies, Cancer Res. 39: 1678–1682.Google Scholar
  57. Rao, B.R., Wiest, W.G., and Allen, W.M., 1974, Progesterone “receptor” in human endometrium, Endocrinology 95: 1275–1281.CrossRefGoogle Scholar
  58. Raynaud, J.P., 1977, R 2050, a tag for the progestin receptor, in: Progesterone Receptors in Normal and Neoplastic Tissues ( W.L. McGuire, J.P. Raynaud, and E.-E. Baulieu, eds.), pp. 9–21, Raven Press, New York.Google Scholar
  59. Raynaud, J.P., Martin, P.M., Bouton, M.M., and Ojasoo, T., 1978, I1ß-Methoxy-17ethynyl-1,3,5(10)-estratriene-3,17ß-diol (Mexestrol, a tag for estrogen receptor binding sites in human tissues, Cancer Res. 38: 3044–3050.Google Scholar
  60. Richardson, G.S., and MacLaughlin, D.T. (eds.), 1978, Hormonal Biology of Endometrial Cancer, UICC Technical Reports Series, Vol. 42, UICC, Geneva.Google Scholar
  61. Robe!, P., Levy, C., Wolff, J.Y., Nicolas, J.C., and Baulieu, E.-E., 1978, Réponse à un antioestrogen comme critère d’hormonosensibilité du cancer de l’endomètre, C. R. Acad. Sci. (Paris) 287: 1353–1356.Google Scholar
  62. Robertson, D.M., Mester, J., Beilby, J., Steele, S.J., and Kellie, A.E., 1971, The measurement of high affinity oestradiol receptors in human uterine endometrium and myometrium, Acta Endocrinol. 68: 534–542.Google Scholar
  63. Sanborn, B.M., Kuo, H.S., and Held, B., 1978, Estrogen and progestagen binding site concentrations in human endometrium and cervix throughout the menstrual cycle and in tissue from women taking oral contraceptives, J. Steroid Biochem. 9: 951–955.CrossRefGoogle Scholar
  64. Schmidt-Gollwitzer, M., Genz, T., Schmidt-Gollwitzer, K., Pollow, B., and Pollow, K., 1978, Correlation between estradiol and progesterone receptor levels, 1713-HSD activity and endometrial tissue levels of estradiol, estrone and progesterone, in: Endometrial Cancer ( M.G. Brush, R.J.B. King, and R.W. Taylor, eds.), Baillière Tindall, London.Google Scholar
  65. Scublinsky, A., Marin, C. and Gurpide, E., 1976, Localization of estradiol l7ß-dehydrogenase in human endometrium, J. Steroid Biochem. 7: 745–747.CrossRefGoogle Scholar
  66. Seal, U.S., and Doe, R.P., 1966, Corticosteroid-binding globulin: Biochemistry, physiology, and phylogeny, in: Steroid Dynamics ( G. Pincus, T. Nakao, and J.F. Tait, eds.), pp. 63–90, Academic Press, New York.Google Scholar
  67. Seematter, R.J., Hoffman, P.G., Kuhn, R.W., Lockwood, L.C., and Siiteri, P.K., 1978, Comparison of 3H-progesterone and (6.7–3H)-17,21-dimethy1–19-norpregna-4,9-diene3,20-dione for the measurement of progesterone receptors in human malignant tissue, Cancer Res. 38: 2800–2806.Google Scholar
  68. Smith, H.E., Smith, R.G., Toft, D.O., Neergard, J.R., Burrows, E.P., and O’Malley, B.W., 1974, Binding of steroids to progesterone receptor proteins in chick oviduct and human uterus, J. Biol. Chem. 249: 5924–5932.Google Scholar
  69. Smith, R.G., Iramain, C.A., Buttram, V.J., Jr, and O’Malley, B.W., 1975, Purification of human uterine progesterone receptor, Nature (London) 253: 271–272.CrossRefGoogle Scholar
  70. Steggles, A.W., and King, R.J.B., 1970, Use of protamine to study 6,7–3H-estradiol-l7ß binding in rat uterus, Biochem. J. 118: 695–701.Google Scholar
  71. Sutherland, R., Mester, J., and Baulieu, E.-E., 1977, Tamoxifen is a potent “pure” anti-oestrogen in chick oviduct, Nature (London) 267: 434–435.CrossRefGoogle Scholar
  72. Sweat, M.L., and Bryson, M.J., 1970, Metabolism of progesterone in proliferative human endometrium and myometrium, Am. J. Obstet. Gynecol. 106: 193–201.Google Scholar
  73. Syrjälä, P., Kontula, K., Jänne, O., Kauppila, A., and Vihko, R., 1978, Steroid receptors in normal and neoplastic uterine tissue, in: Endometrial Cancer ( M.G. Brush, R.J.B. King, and R.W. Taylor, eds.), pp. 242–251, Baillière-Tindall, London.Google Scholar
  74. Trams, G., Engel, B., Lehmann, F., and Maass, H., 1973, Specific binding of oestradiol in human uterine tissue, Acta Endocrinol. 72: 351–360.Google Scholar
  75. Tseng, L., and Gurpide, E., 1972a, Nuclear concentration of estradiol in superfused slices of human endometrium, Am. J. Obstet. Gynecol. 114: 995–1001.Google Scholar
  76. Tseng, L., and Gurpide, E., 1972b, Changes in the in vitro metabolism of estradiol by human endometrium during the menstrual cycle, Am. J. Obstet. Gynecol. 114: 1002–1008.Google Scholar
  77. Tseng, L., and Gurpide, E., 1974, Estradiol and 20a-dihydroprogesterone dehydrogenase activities in human endometrium during the menstrual cycle, Endocrinology 94: 419–423.CrossRefGoogle Scholar
  78. Tseng, L., and Gurpide, E., 1975a, Induction of human endometrial estradiol dehydrogenase by progestins, Endocrinology 97: 825–833.CrossRefGoogle Scholar
  79. Tseng, L., and Gurpide, E., 1975b, Effects of progestins on estradiol receptor levels in human endometrium, J. Clin. Endocrinol. Metab. 41: 402–404.CrossRefGoogle Scholar
  80. Tseng, L., Gusberg, S.B., and Gurpide, E., 1977, Estradiol receptor and l7ß-dehydrogenase in normal and abnormal human endometrium, Ann. N. Y. Acad. Sci. 286: 190–198.CrossRefGoogle Scholar
  81. Tsibris, J.C.M., Cazenave, C.R., Cantor, B., Notelovitz, M., Kalra, P.S., and Spellacy, W.N., 1978, Distribution of cytoplasmic estrogen and progesterone receptors in human endometrium, Am. J. Obstet. Gynecol. 132: 449–454.Google Scholar
  82. Verma, U., and Laumas, K.R., 1973, In vitro binding of progesterone to receptors in the human endometrium and the myometrium, Biochim. Biophys. Acta 317: 403–419.Google Scholar
  83. Vu Hai, M.T., Logeat, F., and Milgrom, E., 1978, Progesterone receptors in the rat uterus: Variations in cytosol and nuclei during the oestrus cycle and pregnancy, J. Endocrinol. 76: 43–49.CrossRefGoogle Scholar
  84. Wiest, W.G., and Rao, B.R., 1971, Progesterone binding proteins in rabbit uterus and human endometrium, in: Advances in the Biosciences, Vol. 7, p. 251, Pergamon Press, Vieweg.Google Scholar
  85. Young, P.C.M., and Cleary, R.E., 1974, Characterization and properties of progesterone-binding components in human endometrium, J. Clin. Endocrinol. Metab. 39: 425–439.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Paul Robel
    • 1
  • Rodrigue Mortel
    • 1
  • Etienne-Emile Baulieu
    • 1
  1. 1.Unité de Recherches sur le Métabolisme Moléculaire et la Physio-PathologieStéroides de l’Institut National de la Santé et de la Recherche Médicale (U 33 INSERM) and ER 125 CNRSBicêtreFrance

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