Abstract
The cells of mammalian nervous tissue divide, differentiate, migrate, and mature in a highly ordered pattern. Each aspect of neuronal development is expressed at a precise time, for a defined purpose. A defect in the regulation of any of the aforenamed steps can result in abnormal nervous tissue. Depending on the type of defective regulation, the stage of development during which it occurs, and the types of cells (neuronal vs. glial) that are involved, an abnormality of nervous tissue could be expressed in the form of malignancy or of various other neurological disorders. Therefore, understanding of the regulation of growth rate, differentiation, migration, maturation, and the expression of individual differentiated functions in nerve cells would be helpful in developing new approaches to the therapy of neurological diseases. Obviously, these problems cannot be systematically investigated in a complex experimental system such as the intact mammalian organism. Techniques of cell culture that allow mammalian nervous tissue to be “simplifed” prior to experimentation provide the possibility of increasing our understanding of these problems.
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© 1981 Plenum Press, New York
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Prasad, K.N. (1981). Cell Culture. In: Lahue, R. (eds) Methods in Neurobiology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3806-2_4
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DOI: https://doi.org/10.1007/978-1-4684-3806-2_4
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