Tissue and Organ Culture

  • Werner T. Schlapfer


The term “tissue culture” has been used to denote the in vitro cultivation and maintenance of living biological materials from multicellular organisms. The techniques that have been developed for this purpose fall into four broad classes: (1) methods to dissociate cells, possibly isolating individual cell types, and to maintain and propagate the dispersed cells as monolayers or suspensions; (2) methods to reassociate previously dissociated cells into aggregates and to maintain these reaggregates in an in vitro environment; (3) methods to cultivate tissue fragments over prolonged periods of time under conditions that encourage cellular interactions as well as differentiation and maturation of the cellular elements in a way resembling the in vivo situation; and (4) methods for maintenance of organ fragments under conditions that discourage the migration of cells from the explant, and that maintain the differentiated cells as a group in a viable, organized, and functioning state. Short-term incubation methods (24 hr or less) of tissue slices or fragments, as are commonly used in biochemical experiments, short-term isolations, or in vitro procedures used in neurophysiology (e.g., isolated ganglia or nerve—muscle preparations), do not qualify as culture systems in the context of this discussion and are not included in this review.


Dorsal Root Ganglion Organ Culture Chick Embryo Nervous Tissue Sympathetic Ganglion 
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Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Werner T. Schlapfer
    • 1
  1. 1.Western Research and Development OfficeVeterans Administration Medical CenterLivermoreUSA

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