Studies of Voltage-Sensitive Sodium Channels in Cultured Cells Using Ion-Flux and Ligand-Binding Methods

  • William A. Catterall


This chapter surveys the development of ion-flux and ligand-binding methods for studies of voltage-sensitive sodium channels and their application to excitable cells in culture. These techniques have proven to be complementary to the electrophysiological methods described in other chapters of this volume in providing new information on the properties of excitable cells in culture and in giving new insight into the fundamental mechanisms of electrical excitability. Electrophysiological methods allow analysis of individual cells; biochemical methods allow analysis of cell populations. Electrophysiological methods give excellent time resolution; biochemical methods are more convenient for pharmacological studies and allow quantitation of ion channel density. One of the principal advantages of cell culture systems for study of electrical excitability is the ability to apply both electrophysiological and biochemical methods in parallel experiments.


Sodium Channel Receptor Site Culture Muscle Cell Scorpion Venom Scorpion Toxin 
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Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • William A. Catterall
    • 1
  1. 1.Department of PharmacologyUniversity of WashingtonSeattleUSA

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