Electrophysiological and Structural Studies of Neurons in Dissociated Cell Cultures of the Central Nervous System

  • Phillip G. Nelson
  • Elaine A. Neale
  • Robert L. MacDonald


The dissociated cell culture approach to the analysis of central nervous system (CNS) function begins with a radical disassembly of the system into its single-cell components. Reconstitution of the system must be done in such a way that the reassembled components convincingly capture those neurobiological properties or processes that are of concern to the investigator. In the most general scheme, this would involve separation of the single cell suspension into pure subpopulations corresponding to each major CNS cell type. The intrinsic properties of each cell type and their development with time could be determined by appropriate electrophysiological, morphological, and biochemical studies. Interactions among the different cell types could then be studied in a variety of cultures formed from combinations of the different cell types. Some of these interactions would presumably be specific to particular cell combinations and would contribute to an understanding of the assembly of specifically organized neuronal systems in the brain.


Spinal Cord Dorsal Root Ganglion Spinal Cord Neuron Postsynaptic Cell Dorsal Root Ganglion Cell 
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Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Phillip G. Nelson
    • 1
  • Elaine A. Neale
    • 1
  • Robert L. MacDonald
    • 2
  1. 1.Laboratory of Developmental Neurobiology, National Institute of Child Health and Human DevelopmentNational Institutes of HealthBethesdaUSA
  2. 2.Department of NeurologyUniversity of MichiganAnn ArborUSA

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