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Serological and Cellular Recognition of Human Histocompatibility Antigens

  • Peter Cresswell
  • Peter E. Andreotti
  • John R. Apgar
  • M. Louise Markert

Abstract

The human major histocompatibility complex (HLA) consists of a region of chromosome 6 (Smith et al., 1976; Francke and Pellegrino, 1977) that contains a number of genes that code for highly polymorphic integral membrane glycoproteins. The allelic products of these genes are generally detected by their reactivity with human alloantisera from multiparous women or from individuals immunized either with lymphocytes (deliberately or by blood transfusions) or by organ grafts. Currently serologically defined are the genes HLA-A, -B, -C, and -DR. HLA-A, -B, and -C code for 44,000-dalton molecules that are expressed noncovalently associated with β 2-microglobulin (Springer and Strominger, 1976; Grey et al., 1973), a 12,000-dalton protein coded for by a gene on chromosome 15 (Good-fellow et al., 1975). HLA-DR molecules on the surface of B-lymphoid cells and monocytes consist of two noncovalently associated glycoproteins with approximate molecular weights of 35,000 and 27,000 (Cress-well and Geier, 1975; Springer et al., 1977). Which of these molecules is the true product of the HLA-DR gene is not absolutely certain; conflicting evidence suggests that both the isolated 27,000-dalton and the 35,000-dalton molecule can bind alloantibodies to HLA-DR following denaturation and separation (Tosi et al., 1978; Klareskog et al., 1978).

Keywords

Cytotoxic Cell Mutant Cell Line Human Major Histocompatibility Complex Integral Membrane Glycoprotein Cellular Recognition 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Ayres, J., and Cresswell, P., 1976, HLA-B specificities and w4, w6 specificities are on the same polypeptide, Eur. J. Immunol. 6:794.PubMedCrossRefGoogle Scholar
  2. Bright, S., 1976, Second locus HL-A antigens and 4a or 4b, Tissue Antigens 7:23.PubMedCrossRefGoogle Scholar
  3. Chess, L., MacDermott, R.P., and Schlossman, S.F., 1974, Immunologic function of isolated human lymphocyte subpopulations. II. Antigen triggering of T and B cells in vitro, J. Immunol. 113:1122.Google Scholar
  4. Cook, R.G., Vitetta, E.S., Uhr, J.W., and Capra, J.D., 1979, Structural studies on the murine Ia alloantigens. V. Evidence that the structural gene for the I-E/C beta polypeptide is encoded within the I-A subregion, J. Exp. Med. 149:981.PubMedCrossRefGoogle Scholar
  5. Cresswell, P., 1977, Human B cell alloantigens: Separation from other membrane molecules by affinity chromatography, Eur. J. Immunol. 7:636.PubMedCrossRefGoogle Scholar
  6. Cresswell, P., and Ayres, J.L., 1976, HLA antigens: Rabbit antisera reacting with all A series or all B series specificities, Eur. J. Immunol. 6:82.PubMedCrossRefGoogle Scholar
  7. Cresswell, P., and Geier, S.S., 1975, Antisera to human B-lymphocyte membrane glycoproteins block stimulation in mixed lymphocyte culture, Nature(London) 257:147.CrossRefGoogle Scholar
  8. Dorval, G., Welsh, K.I., and Wigzell, H., 1974, Labeled staphylococcal protein A as an immunological probe in the analysis of cell surface markers, Scand. J. Immunol. 3:405.PubMedCrossRefGoogle Scholar
  9. Francke, U., and Pellegrino, M.A., 1977, Assignment of the major histocompatibility complex to a region of the short arm of human chromosome 6, Proc. Natl. Acad. Sci. U.S. A. 74:1147.PubMedCrossRefGoogle Scholar
  10. Goodfellow, P.N., Jones, E.A., van Heyningen, V., Solomon, E., Bobrow, M., Miggiano, V., and Bodmer, W.F., 1975, The β 2-microglobulin gene is on chromosome 15 and not in the HL-A region, Nature(London) 254:267.CrossRefGoogle Scholar
  11. Grey, H.M., Kubo, R.T., Colon, S.M., Poulik, M.D., Cresswell, P., Springer, T., Turner, M.J., and Strominger, J.L., 1973, The small subunit of HL-A antigens is β 2-microglobulin, J. Exp. Med. 138:1608.PubMedCrossRefGoogle Scholar
  12. Jones, P.P., 1977, Analysis of H-2 and Ia molecules by two-dimensional gel electrophoresis, J. Exp. Med. 146:1261.PubMedCrossRefGoogle Scholar
  13. Jones, P.P., Murphy, D.B., and McDevitt, H.O., 1978, Two-gene control of the expression of a murine Ia antigen, J. Exp. Med. 148:925.PubMedCrossRefGoogle Scholar
  14. Klareskog, L., Rask, L., Fohlman, J., and Peterson, P.A., 1978, Heavy HLA-DR (Ia) antigen chain is controlled by the MHC region, Nature (London) 275:762.CrossRefGoogle Scholar
  15. Kristensen, T., Grunnet, N., and Kissmeyer-Nielsen, F., 1975, Cell mediated lympholysis in man: Varying strength of the HL-A (LA and Four) antigens as sensitizing or target determinants, Tissue Antigens 6:221.PubMedCrossRefGoogle Scholar
  16. Legrand, L., and Dausset, J., 1975, The complexity of the HL-A gene product. II. Possible evidence for a “public” determinant common to the first and second HL-A series, Transplantation 19:177.PubMedCrossRefGoogle Scholar
  17. Lohrmann, H.P., Novikovs, L., and Graw, R.G., 1974, Stimulatory capacity of human T and B lymphocytes in the mixed leukocyte culture, Nature (London) 250:144.CrossRefGoogle Scholar
  18. McMichael, A.J., Ting, A., Zweerink, H.J., and Askonas, B.A., 1977, HLA restriction of cell-mediated lysis of influenza virus-infected human cells, Nature (London) 270:524.CrossRefGoogle Scholar
  19. O’Farrell, P.H., 1975, High resolution two-dimensional electrophoresis of proteins, J. Biol. Chem. 250:4007.PubMedGoogle Scholar
  20. Parham, P., Albert, B.N., Orr, H.T., and Strominger, J.L., 1977, Carbohydrate moiety of HLA antigens—Antigenic properties and amino acid sequences around the site of glycosylation, J. Biol. Chem. 252:7555.PubMedGoogle Scholar
  21. Pious, D., Hawley, P., and Forrest, G., 1973, Isolation and characterization of HL-A variants in cultured human lymphoid cells, Proc. Natl. Acad. Sci. U.S.A. 70:1397.PubMedCrossRefGoogle Scholar
  22. Pious, D., Soderland, C., and Gladstone, P., 1977, Induction of HLA mutations by chemical mutagens in human lymphoid cells, Immunogenetics 4:437.CrossRefGoogle Scholar
  23. Robinson, M.A., Noreen, H.J., Amos, D.B., and Yunis, E.J., 1978, Target antigens of cell-mediated lympholysis: Discrimination of HLA subtypes by cytotoxic lymphocytes, J. Immunol. 121:1486.PubMedGoogle Scholar
  24. Silver, J., Walker, L.E., Reisfeld, R.A., Pellegrino, M.A., and Ferrone, S., 1979, Structural studies of murine I-E and human DR antigens, Mol. Immunol. 16:37.PubMedCrossRefGoogle Scholar
  25. Smith, M., Gold, P., Shuster, J., Tanigaki, N., and Pressman, D., 1976, Chromosomal assignment of the HL-A common antigenic determinants in man-mouse somatic cell hybrids, J. Immunogenet. 3:105.PubMedCrossRefGoogle Scholar
  26. Springer, T.A., and Strominger, J.L., 1976, Detergent-soluble HLA antigens contain a hydrophilic region at the COOH-terminus and a penultimate hydrophobic region, Proc. Natl. Acad. Sci. U.S.A. 73:2481.PubMedCrossRefGoogle Scholar
  27. Springer, T.A., Kaufman, J.F., Terhorst, C., and Strominger, J.L., 1977, Purification and structural characterisation of human HLA-linked B cell antigens, Nature (London) 268:213.CrossRefGoogle Scholar
  28. Tosi, R., Tanigaki, N., Centis, D., Ferrara, G.B., and Pressman, D., 1978, Immunological dissection of human Ia molecules, J. Exp. Med. 148:1592.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1981

Authors and Affiliations

  • Peter Cresswell
    • 1
  • Peter E. Andreotti
    • 1
  • John R. Apgar
    • 1
  • M. Louise Markert
    • 1
  1. 1.Division of Immunology, Department of Microbiology and ImmunologyDuke University Medical CenterDurhamUSA

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