HLA Polyclonal and Monoclonal Xenoantibodies

Production, Characterization, and Application to the Study of HLA Antigens
  • Barry S. Wilson
  • Ah-Kau Ng
  • Vito Quaranta
  • Soldano Ferrone


A myriad of cellular functions are mediated through cell-cell interactions and are a result of communication by surface-membrane structures; therefore, these structures, particularly human histocompatibility (HLA) antigens and their functions, are the subjects of intense analysis. The characterization of these membrane components would be quite difficult were it not that most of these molecules are antigenic and elicit the production of specific antibodies through allo- or xenoimmunization. Alloimmunization produces antibodies to antigenic determinants that are polymorphic within a species (alloantigens); the latter method produces antibodies to these determinants as well as to species-specific determinants. Virtually all allo- and xenoantisera contain multiple populations of antibodies that differ in affinity, specificity, and titer, and are the products of distinct clones of cells. These conventional allo- and xenoantisera will be referred to as polyclonal antisera.


Heavy Chain Fab2 Fragment Human Complement Common Determinant Daudi Cell 
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Copyright information

© Springer Science+Business Media New York 1981

Authors and Affiliations

  • Barry S. Wilson
    • 1
  • Ah-Kau Ng
    • 1
  • Vito Quaranta
    • 1
  • Soldano Ferrone
    • 1
  1. 1.Department of ImmunologyScripps Clinic and Research FoundationLa JollaUSA

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