In Vivo Quantitation of Induced Ultrasonic Contrast Effects in the Mouse
The ability of certain aqueous solutions to enhance ultrasonic echogenicity from the intact mouse in vivo was examined. Substances which in solution increase the velocity of sound as a function of increasing concentration were found to intensify the “contrast” of whole mice visualized sonographically. Increase in contrast was determined by photometric measurement of the average integrated screen luminance of the area of the screen representing the mouse. The ability to enhance screen luminance correlated with the ratio of the slope of the change in speed of sound vs. concentration to the maximum non-lethal drug dose attainable, as determined by acute LD50 studies. Neither body temperature nor osmolarity changes appeared to be significant contributing factors in the enhancement of “contrast” produced by the agents tested. These results indicate that compounds with a high slope of the change of speed of sound vs. concentration in solution and with low toxicity can act in vivo as ultrasonic “contrast” agents when given systemically.
KeywordsSodium Citrate Contrast Effect Calcium Gluconate Median Lethal Dose Disodium Edetate
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