Abstract
Though large vessel endothelium has been studied extensively in vitro as an isolated cell type, microvascular endothelium has been studied in vitro on only a very limited basis, usually as whole microvessel isolates (1–5). This is due mainly to the difficulty of isolating and maintaining pure microvascular endothelium, though some attempts have been made (4,6–8). In a recent study (9) we isolated microvessels from mouse cerebrum and described a culture procedure for obtaining endothelial and smooth muscle cells in primary culture. We also have data on the establishment of an endothelial cell line, designated ME-2, derived from similar microvessel explants (10). This report describes, in greater detail, some of the properties or characteristics of the ME-2 endothelial cell line.
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© 1980 Plenum Press, New York
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DeBault, L.E., Cancilla, P.A. (1980). Some Properties of Isolated Endothelial Cells in Culture. In: Eisenberg, H.M., Suddith, R.L. (eds) The Cerebral Microvasculature. Advances in Experimental Medicine and Biology, vol 131. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3752-2_6
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DOI: https://doi.org/10.1007/978-1-4684-3752-2_6
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