Normal and Altered Regional Cerebral Blood Flow and Solute Movement Across the Cerebral Capillary in Rat

  • M. Pollay
  • A. Stevens
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 131)


Prior to the pioneering efforts of Crone (1), the integrity of the blood-brain barrier was measured by the quantity of labeled protein entering brain tissue after its introduction into blood. The indicator diffusion technique presented by Crone allowed not only an evaluation of the exclusionary properties of the blood-brain barrier (BBB), but, more importantly, the specialized transport systems operating across this barrier. This technique measures the single pass extraction of a diffusible solute in transit through cerebral capillary beds as compared to a reference compound which normally is excluded from the brain. This experimental method measures events involving the entire cerebrum and its precision is effected by interlaminar diffusion (Taylor effect). Modifications of the indicator dilution technique have been utilized to demonstrate the carrier- mediated transport of a number of sugars and amino acids across the blood-brain barrier (2).


Cerebral Blood Flow Blood Flow Measurement Brain Capillary Tritiated Water Back Diffusion 
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Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • M. Pollay
    • 1
  • A. Stevens
    • 1
  1. 1.Neurosurgery SectionUniversity of Oklahoma Health Sciences Center and Research Service, Oklahoma Veterans Administration HospitalOklahoma CityUSA

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