The Effect of Severe Hypoxia on Cerebral Glucose Flux

  • D. D. Gilboe
  • D. Costello
  • J. H. FitzpatrickJr.
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 131)


We have already studied the changes that result in altered glucose transport and metabolism when the isolated canine brain is perfused with blood having a normal glucose concentration, but an arterial PO2 of less than 10 mmHg (1,2). After the first minute following initiation of anoxic perfusion, the rate of glucose utilization increases and, at normal arterial glucose concentrations, actually exceeds the capacity for glucose to be transported across the blood-brain barrier (BBB). The result is that the whole brain glucose concentration rapidly falls to less than 24% of normal after 10 minutes of anoxic perfusion and to nearly zero after a total of 30 minutes of anoxic perfusion (3).


Glucose Concentration Glucose Transport Capillary Endothelial Cell Brain Glucose Glucose Flux 


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  1. 1.
    Betz AL, Gilboe DD, Drewes LR: Effects of anoxia on net uptake and unidirectional transport of glucose into the isolated dog brain. Brain Res 67: 307–316, 1974.CrossRefPubMedGoogle Scholar
  2. 2.
    Betz AL, Gilboe DD, Drewes LR: Accelerative exchange diffusion kinetics of glucose between blood and brain and its relation to transport during anoxia. Biochim Biophys Acta 401: 416–428, 1975.CrossRefPubMedGoogle Scholar
  3. 3.
    Drewes LR, Gilboe DD, Betz AL: Metabolic alterations in brain during anoxic-anoxia and subsequent recovery. Arch Neurol 29: 385–390, 1973.CrossRefPubMedGoogle Scholar
  4. 4.
    Gilboe DD, Drewes LR, Kintner D: Edema formation in the isolated dog brain: Anoxia vs ischemia. In, Pappius H and Feindel W (eds): Dynamics of Brain Edema, New York, Springer-Verlay, 1976, pp 228–235.Google Scholar
  5. 5.
    Spatz M, Klatzo I: Pathological aspects of brain transport phenomena. Adv Exp Biol Med 69: 479–495, 1976.CrossRefGoogle Scholar
  6. 6.
    KJjitner D, Costello DJ, Gilboe DD: Brain metabolism following thirty minutes of hypoxic or anoxic perfusion, or ischemia. Am J Physiol, In press.Google Scholar
  7. 7.
    Drewes LR, Gilboe DD: Glycolysis and the permeation of glucose and lactate in the isolated, perfused dog brain during anoxia and post-anoxic recovery. J Biol Chem 218: 2489–2496, 1973.Google Scholar
  8. 8.
    Betz AL, Gilboe DD, Drewes LR: Kinetics of unidirectional leucine transport into brain: Effects of isoleucine, valine and anoxia. Am J Physiol 228: 895–900, 1975.PubMedGoogle Scholar
  9. 9.
    Gilboe DD, Betz AL, Langebartel DA: A guide for the isolation of the canine brain. J Appl Physiol 34: 534–537, 1973.PubMedGoogle Scholar
  10. 10.
    Gilboe DD, Andrews RL, Dardenne G: Factors affecting glucose uptake by the isolated dog brain. Am J Physiol 219: 767–773, 1970.PubMedGoogle Scholar
  11. 11.
    Betz AL, Gilboe DD, Yudilevich DL, et al: Kinetics of uni directional glucose transport into the isolated dog. Am J Physiol 225: 586–592, 1973.PubMedGoogle Scholar
  12. 12.
    Cleland WW: The statistical analysis of enzyme kinetic data. Adv Enzymol 29: 1–32, 1967.PubMedGoogle Scholar
  13. 13.
    Drewes LR, Frazin L, Levin A: Blood flow in the isolated, perfused canine brain: Normal oxygenation vs anoxic anoxia. Physiologist 18: 198, 1975.Google Scholar
  14. 14.
    Bloch R: Human erythrocyte sugar transport kinetic evidence for an asymmetric carrier. J Biol Chem 249: 3543–3550, 1974.PubMedGoogle Scholar
  15. 15.
    Levine M, Oxender DL, Stein WD: The substrate-facilitated transport of the glucose carrier across the human erythrocyte membrane. Biochim Biophys Acta 109: 151–163, 1965.CrossRefPubMedGoogle Scholar
  16. 16.
    Lund-Anderson H: Transport of glucose from blood to brain. Physiol Rev 59: 305–352, 1979.Google Scholar
  17. 17.
    Buschiazzo PM, Terrell EB, Regen DM: Sugar transport across the blood-brain barrier. Am J Physiol 219: 1505–1513, 1970.PubMedGoogle Scholar
  18. 18.
    Crone C: Facilitated transfer of glucose from blood into brain tissue. J Physiol (Lond) 181: 103–113, 1965.CrossRefGoogle Scholar
  19. 19.
    Oldendorf WH: Brain uptake of radiolabeled amino acids, amines and hexoses after arterial injection. Am J Physiol 221: 1629–1639, 1971.PubMedGoogle Scholar
  20. 20.
    Pappenheimer JR, Setchell BP: Cerebral glucose transport and oxygen consumption in sheep and rabbits. J Physiol 233: 529–551, 1973.CrossRefPubMedPubMedCentralGoogle Scholar
  21. 21.
    Oldendorf WH, Brown WS: Greater numbers of capillary endothelial cell mitochondria in brain than muscle. Proc Soc Exp Biol Med 149: 736–738, 1975.CrossRefPubMedGoogle Scholar
  22. 22.
    Drewes LR, Remick DG: Uncoupling of brain respiratory metabolism by pentachlorophenol. Fed Proc 37: 1628, 1978.Google Scholar
  23. 23.
    Sen AK, Widdas WF: Determination of the temperature and Ph dependence of glucose transfer across the human erythrocyte membrane measured by glucose exit. J Physiol 160: 392–403, 1962.CrossRefPubMedPubMedCentralGoogle Scholar
  24. 24.
    Ljunggren B, Schutz H, Siesjo BK: Changes in energy state and acid-base parameters of the rat brain during complete compression ischemia. Brain Res 73: 277–289, 1974.CrossRefPubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • D. D. Gilboe
    • 1
    • 2
  • D. Costello
    • 1
    • 2
  • J. H. FitzpatrickJr.
    • 1
    • 2
  1. 1.Department of NeurosurgeryUniversity of Wisconsin Medical SchoolMadisonUSA
  2. 2.Department of PhysiologyUniversity of Wisconsin Medical SchoolMadisonUSA

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