Immobilization of Biocatalysts Using Ultrafiltration Techniques
Continuous operation with a coenzyme dependent multi-enzyme-system is possible in a membrane reactor, when a soluble polymer-bound coenzyme is used. The soluble enzymes and the soluble artificially enlarged coenzyme are retained by means of an ultrafiltration membrane acting as a frit, while the reactants of low molecular weight are forced across the membrane by means of a conveying pump. Thus, a continuous homogeneous catalysis system using a coenzyme as transport metabolite can be established.
KeywordsMembrane Reactor Soluble Enzyme Continuous Stir Tank Reactor Soluble Polymer Ultrafiltration Membrane
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- 1.Gesellschaft fur Biotechnologische Forschung, “Verfahren zur Herstellung von an Makromolekule gebundenen, ein Andeninringsystem enthaitended Coenzymen” Deutsche Auslegeschrift (DE-AS) Nr. 28 41 414, 1979.Google Scholar