The cross-linking of the polysaccharides with epichlorhydrin has been known since early in this century. However, the acidic polysaccharides such as pectate and alginate do not behave as the neutral polydextrans or agarose. So, a systematic program was undertaken to study this reaction and a critical cross-linking allowed us to prepare an affinity matrix for the polygalacturonase (Pectalihydrolase) purification (1). Further a methanol ammonia treatment modifying the COOH-groups of the cross-linked pectate, provided a second affinity matrix for the Pectinesterase purification (2).
KeywordsBiological Activity Yeast Strain Affinity Chromatography Cell Immobilization Flow Property
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- 2.VIJAYALAKSHMI, M.A., et al. “Affinity chromatography” (O. Hoffman-Ostenhof, Ed.) Pergamon Press, New York, 1978.Google Scholar
- 3.VIJAYALAKSHMI, M. & Porath, J. to be published in J. Chromatography, 1979.Google Scholar