Cross-Linked Pectate

  • Mookambeswaran A. Vijayalakshmi
  • Daniel Picque
  • Georges B. Broun


The cross-linking of the polysaccharides with epichlorhydrin has been known since early in this century. However, the acidic polysaccharides such as pectate and alginate do not behave as the neutral polydextrans or agarose. So, a systematic program was undertaken to study this reaction and a critical cross-linking allowed us to prepare an affinity matrix for the polygalacturonase (Pectalihydrolase) purification (1). Further a methanol ammonia treatment modifying the COOH-groups of the cross-linked pectate, provided a second affinity matrix for the Pectinesterase purification (2).


Biological Activity Yeast Strain Affinity Chromatography Cell Immobilization Flow Property 
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  1. 1.
    VIJAYALAKSHMI, M.A., et al. Lebens. Wiss. u. Tekhn. 11: 288–290, 1978.Google Scholar
  2. 2.
    VIJAYALAKSHMI, M.A., et al. “Affinity chromatography” (O. Hoffman-Ostenhof, Ed.) Pergamon Press, New York, 1978.Google Scholar
  3. 3.
    VIJAYALAKSHMI, M. & Porath, J. to be published in J. Chromatography, 1979.Google Scholar
  4. 4.
    VIJAYALAKSHMI, M.A., et al. Annals N.Y. Academy of Sciences, 326, 1979, pp. 249–254.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Mookambeswaran A. Vijayalakshmi
    • 1
  • Daniel Picque
    • 1
  • Georges B. Broun
    • 1
  1. 1.Institut de Technologie des Surfaces ActivesUniversite de Technologie de CompiegneCompiegneFrance

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