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Enzyme Immobilization on Pyridine Containing Polymers

  • F. Pittner
  • T. Miron
  • G. Pittner
  • M. Wilchek

Abstract

The reaction of the nucleophilic pyridine nitogen with strong electrophiles such as cyanogen bromide, fluorodinitrobenzene and p-toluene-sulfonyl chloride to yield glutaconic aldehyde is well known (1,2). This reaction was the base for a quantitative method for the determination of Cyanids (3), and it was recently used as a tool for the following of activation of polysaccharides with cyanogen bromide (4). In the following, we describe that polymers containing pyridine can be also converted to polyaldehydes (glutaconic aldehyde) by the reaction with cyanogen bromide. This polyaldehyde can be used directly for the binding of protein and small ligands.

Keywords

Glass Bead Enzyme Immobilization Barbituric Acid Pyridine Derivative Weizmann Institute 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Reference

  1. 1.
    KONIG, W. J. Prakt. Chem. 69: 105–110, 1904.CrossRefGoogle Scholar
  2. 2.
    SCHWARZENBACH, G. & WEBER, R. Helv. Chim. Acta. 25: 1628–1635, 1942.CrossRefGoogle Scholar
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    ASMüS, E. & GARSCHAGEN, H. Z. Anal. Chem.138: 414–418, 1953.CrossRefGoogle Scholar
  4. 4.
    KOHN, J. & WILCHEK, M. Biochem. Blophys. Res. Comm. 84: (1) 7–14, 1978.CrossRefGoogle Scholar
  5. 5.
    WEETALL, H.H. in Methods in Enzymology XLIV, Academic Press, New York, 1976, p. 139.Google Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • F. Pittner
    • 1
  • T. Miron
    • 1
  • G. Pittner
    • 1
  • M. Wilchek
    • 1
  1. 1.Department of BiophysicsThe Weizmann Institute of ScienceRehovotIsrael

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