Preparation and Characterization of Metal Oxide-Coated Supports for Oxidase Enzymes
In our early studies on the immobilization of L-amino acid oxidase (1), we co-immobilized catalase in order to decompose H2O2 that is produced in the primary reaction of L-amino acids to α-keto acids. The catalase activity was lost relatively rapidly, leading to a loss in oxidase activity. This problem led us to investigate other methods for achieving in situ degradation of H2O2 by non-enzymatic catalysis. Similar approaches to this problem have also been investigated recently using glucose oxidase (2–4) and D-amino acid oxidase (5) systems.
KeywordsManganese Oxide Cobalt Oxide Acid Oxidase Oxidase Enzyme Ruthenium Oxide
Unable to display preview. Download preview PDF.
- 1.Fink, D.J., Falb, R.D. & Bean, M.K. A.I.Ch.E. Sum. Ser. No. 127, 74: 18, 1978.Google Scholar
- 3.Cho, Y.K. & Bailey, J.E. Biotechnol. Bioeng. 19: 769:775, 1977.Google Scholar
- 4.Carter, R. in “Enzyme Engineering”, Vol. 5 (H. H. Weetall and G. P. Royer, eds.), Plenum Press, New York, 1979, p.Google Scholar
- 5.BRODELIUS, P. ibid, p.Google Scholar
- 7.Bergmeyer, H.U. & Bernt, E. in “Methods of Enzymatic Analysis,” Vol. 3, 2nd English Edition (H. U. Bergmeyer, ed.), Academic Press, New York, 1974, p. 1212.Google Scholar