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Distribution of Staphylococcal Nuclease Insolubilized on Sepharose

  • Jose M. Guisan
  • Victor M. Fernandez
  • Antonio Ballesteros

Abstract

We have studied the activity of micrococcal nuclease (EC 3.1.4.7, MW 16,800) insolubilized on CNBr-activated Sepahrose toward a high molecular weight substrate (heat-denatured DNA, MW around 500,000). The nuclease was immobilized directly through its amino groups on the activated Sepharose. (The derivatives prepared were the best as compared with others incorporating diazo or ethylene spacers (1)).

Keywords

Enzyme Concentration Outer Part Fluorescein Isothiocyanate Coupling Yield Enzyme Molecule 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Reference

  1. 1.
    GUISAN, J.M. & BALLESTEROS, A. J. Solid-Phase Biochem., in press (1979).Google Scholar
  2. 2.
    GUISAN, J.M & BALLESTEROS, A. in “Enzyme Engineering” Vol. 4 (G. B. Broun, G. Manecke and L. Wingard, Jr., eds.) Plenum Press, New York, 1978, p. 117.Google Scholar
  3. 3.
    KOHN, J. & WILCHEK, M. Blochem. Blophys. Res. Comm.84: 7, 1978.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Jose M. Guisan
    • 1
  • Victor M. Fernandez
    • 1
  • Antonio Ballesteros
    • 1
  1. 1.Consejo Superior de Investigaciones CientificasInstituto de Catalisis y PetroleoquimicaMadridSpain

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