Distribution of Staphylococcal Nuclease Insolubilized on Sepharose
We have studied the activity of micrococcal nuclease (EC 22.214.171.124, MW 16,800) insolubilized on CNBr-activated Sepahrose toward a high molecular weight substrate (heat-denatured DNA, MW around 500,000). The nuclease was immobilized directly through its amino groups on the activated Sepharose. (The derivatives prepared were the best as compared with others incorporating diazo or ethylene spacers (1)).
KeywordsEnzyme Concentration Outer Part Fluorescein Isothiocyanate Coupling Yield Enzyme Molecule
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