Cloning of the Penicillin G-Acylase Gene of Escherichia Coli ATCC 11105 on Multicopy Plasmids

  • H. Mayer
  • J. Collins
  • F. Wagner


The great advances in modern genetic methodology, so-called “genetic engineering” has so far found little application in the development of improved strains, i.e. strains which could compete with organisms currently used in biotechnological processes.


Phenylacetic Acid Penicillin Versus Penicillin ACYLASE Acylase Activity Sodium Phenylacetate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    ROLINSON, G.N., BATCHELOR, F.R., BUTTERWORTH, D., CAMERON-WOOD, J., COLE, M., EUSTACHE, G.C., HART, M.V., RICHARDS, M. & CHAIN, E.B. Nature187: 236–237, 1960.CrossRefGoogle Scholar
  2. 2.
    KUTZBACH, C. & RAUENBAC, E. Hoppe-Seyler’s Z. Physiol. Chem.354: 45–53, 1974.CrossRefGoogle Scholar
  3. 3.
    COLLINS, J. & HOHN, B. Proc. Natl. Acad. Scie. USA 9: 4242–4246, 1978.CrossRefGoogle Scholar
  4. 4.
    COLLINS, J. & BRUNING, H.J. Gene 4: 85–107, 1978.CrossRefGoogle Scholar
  5. 5.
    BOLIVAR, F., RODRIGUEZ, R.L., GREENE, P.J., BETLACH, M.C., HEYNEKER, H.L., BOYER, H.W., CROSA, J.H. & FALKOW, S. Gene2: 95–113, 1977.CrossRefGoogle Scholar
  6. 6.
    OOSTENDORP, I.G. Antonie van Leeuwenhoek38: 201, 1972.CrossRefGoogle Scholar
  7. 7.
    BOMSTEIN, J. & EVANS, W.G. Anal. Chem. 37: 576, 1965.CrossRefGoogle Scholar
  8. 8.
    SCHNEIDER, W.J. & ROEHR, M. Biochim. Biophys. Acta452: 177–185, 1976.Google Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • H. Mayer
    • 1
  • J. Collins
    • 1
  • F. Wagner
    • 2
  1. 1.Gesellschaft fur Biotechnologische Forschung mbHBraunschweigGermany
  2. 2.Lehrstuhl fur Biotechnologie und BiochemieTechnische Universitat BraunschweigBraunschweigW. Germany

Personalised recommendations