Synthesis of Coenzymes by Immobilized Cell System

  • Hideaki Yamada
  • Sakayu Shimizu
  • Yoshiki Tani
  • Tsunetoshi Hino


Recent developments in the utilization of coenzymes for medicine, in animal feed and as reagents for biochemical analysis have raised interest in the economical production of coenzymes and brought a number of papers on preparative methods for coenzymes. In previous papers (1–4), we revealed that such useful coenzymes as CoA, FAD and pyridoxal 5′-phosphate can conveniently and efficiently be synthesized by enzymatic processes which employ microbial cells as enzyme sources. In the present paper, we describe the immobilization of these microbial cells and the continuous synthesis of these coenzymes by the immobilized cells as stable catalysts.


Immobilize Cell Sodium Laurylsulfate Pantothenic Acid Substrate Mixture Continuous Synthesis 
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  1. 1.
    OGATA, K. in “Advances in Applied Microbiology,” Vol. 19 (D. Perlman, ed.) Academic Press, New York, 1975, p. 209.Google Scholar
  2. 2.
    SHIMIZU, S., TANI, Y. & OGATA, K. in “Methods in Enzymology,” Vol. 62 (D. B. McCormick & L. D. Wright, Eds.) Academic Press, New York, 1979, p. 236.Google Scholar
  3. 3.
    SHIMIZU, S., YAMANE, K., TANI, Y. & YAMADA, H. in “Abstr. Annu. Mtg. Soc. Ferment. Technol., Japan, “1977, p. 60.Google Scholar
  4. 4.
    TANI, Y., KAWATA, S., TOCHIKURA, T. & OGATA, K. Agric. Biol. Chem. 33: 314, 1969.CrossRefGoogle Scholar
  5. 5.
    OGATA, K., SHIMIZU, S. & TANI, Y. Agric. Biol. Chem. 34: 1757, 1970.CrossRefGoogle Scholar
  6. 6.
    SHIMIZU, S., MIYATA, K., TANI, Y. & OGATA, K. Agric. Biol. Chem. 37: 607, 1973.CrossRefGoogle Scholar
  7. 7.
    SHIMZU, S., MORIOKA, H., TANI, Y. & OGATA, K. J. Ferment. Technol. 53: 77, 1975.Google Scholar
  8. 8.
    SHIMZU, S.., KUBO, K., TANI, Y. & OGATA, K. Agric. Biol. Chem. 37: 2863, 1973.CrossRefGoogle Scholar
  9. 9.
    SHIMIZU, S., KUBO, K., MORIOKA, H., TANI, Y. & OGATA, K. Agric. Biol. Chem. 38: 1015, 1974.CrossRefGoogle Scholar
  10. 10.
    SHIMIZU, S., YAMANE, K., TANI, Y., YAMADA, H., HINO, T. & OKAMURA, S. in “Abstr. 308th Mtg. Agric. Chem. Soc. Japan (Kansai Division),” 1978, p. 2.Google Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Hideaki Yamada
    • 1
  • Sakayu Shimizu
    • 1
  • Yoshiki Tani
    • 1
  • Tsunetoshi Hino
    • 2
  1. 1.Department of Agricultural ChemistryKyoto UniversityKyoto 606Japan
  2. 2.Research Institute for Production DevelopmentKyoto 606Japan

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