Enzymatic Synthesis of Pantothenic Acid by Escherichia Coli Cells

  • Y. Kawabata
  • A. L. Demain


Pantothenic acid is a vitamin of importantance for human and animal nutrition. At present, it is commerically produced by chemical synthesis. Pantothenic acid is naturally produced from pantioc acid and (S-alanine by most microbial species, its formation being catalyzed by pantothenic acid synthetase (E.C. (1,2). This enzyme required ATP which is converted to AMP during the enzyme reaction. It has been difficult to use these ATP-requiring enzymes in industry because of the cost of ATP. Attempts have been made to devise processes for ATP regeneration. One of these is the enzymatic regeneration of ATP from ADP using adenylate kinase, acetate kinase and acetyl phosphate (3,4). This method has been applied to the synthesis of gramicidin S by a cell-free enzyme system (5). This paper describes the enzymatic synthesis of pantothenic acid by Escherichia coli cells using ATP regeneration.


Enzymatic Synthesis Pantothenic Acid Adenylate Kinase Acetate Kinase Acetyl Phosphate 
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Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Y. Kawabata
    • 1
  • A. L. Demain
    • 1
  1. 1.Department of Nutrition and Food ScienceMassachusetts Institute of TechnologyCambridgeUSA

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