Abstract
Pantothenic acid is a vitamin of importantance for human and animal nutrition. At present, it is commerically produced by chemical synthesis. Pantothenic acid is naturally produced from pantioc acid and (S-alanine by most microbial species, its formation being catalyzed by pantothenic acid synthetase (E.C. 6.3.2.1) (1,2). This enzyme required ATP which is converted to AMP during the enzyme reaction. It has been difficult to use these ATP-requiring enzymes in industry because of the cost of ATP. Attempts have been made to devise processes for ATP regeneration. One of these is the enzymatic regeneration of ATP from ADP using adenylate kinase, acetate kinase and acetyl phosphate (3,4). This method has been applied to the synthesis of gramicidin S by a cell-free enzyme system (5). This paper describes the enzymatic synthesis of pantothenic acid by Escherichia coli cells using ATP regeneration.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
MAAS, W.K. J. Biol. Chem. 198: 23, 1952.
BROWN, G.M. in “Comprehensive Biochemistry,” Vol. 21 (M. Florkin and E. H. Stolty, eds.) Elsevier, Amsterdam, 1971, p. 75.
GARDNER, C. R., COLTON, C.K., LANGER, R.S., HAMILTON, B.K., ARCHER, M.C. & WHITESIDES, G.M. in “Enzyme Engineering,” Vol. 2 (E. K. Pye and L. B. Wingard, Jr., eds.) Plenum Press, New York, 1974, p. 209.
WHITESIDES, G. M., CHMURNY, A., GARRETT, P., LAMOTTE, A. & COLTON, C.K. in “Enzyme Engineering,” Vol. 2 (E. K. Pye and L. B. Wingard, Jr., eds.) Plenum Press, New York, 1974, p. 217.
STRAMONDO, J.G., SOLOMON, B.A., COLTON, C.K. & WANG, D.I.C., Alche Symposium Series 74: No. 12, 1, 1978.
MIOZZARI, G.F., NIEDERBERGER, P & HUETTER, R. Anal. Biochem. 90: 220, 1978.
KOKIMA, H., MATSUYA, Y., OZAWA, H., KONNO, M. & UEMURA, T., J. Agr. Chem. Soc. (Japan) 32: 33, 1958.
KAWABATA, Y. & DEMAIN, A. L. in “Immobilized Microbial Cells,” ACS Symposium Series No. 106, (K. Venkatsubramanian, ed.) American Chemical Society, Washington D. C., 1979, p. 133.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1980 Plenum Press, New York
About this chapter
Cite this chapter
Kawabata, Y., Demain, A.L. (1980). Enzymatic Synthesis of Pantothenic Acid by Escherichia Coli Cells. In: Weetall, H.H., Royer, G.P. (eds) Enzyme Engineering. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3749-2_63
Download citation
DOI: https://doi.org/10.1007/978-1-4684-3749-2_63
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-3751-5
Online ISBN: 978-1-4684-3749-2
eBook Packages: Springer Book Archive