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The use of Free and Immobilized Cells in the Presence of Organic Solvents: The Oxidation of Cholesterol by Nocardia Rhodochrous

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Enzyme Engineering

Abstract

We have been using Nocardia rhodochrous to oxidize cholesterol. Here we report the oxidation of cholesterol to cholestenone with free and immobilized cells induced for maximum cholesterol oxidase activity. Cholesterol, an almost water-insoluble substrate, is solubilized in a water-immiscible organic solvent. In such a heterogeneous system the reaction rates are dependent on several factors including the substrate concentrations, the amount of catalysts and the organic/aqueous ratio. Also the nature of the solvent can influence the observed rates by affecting the enzyme environment and/or the accessibilities of the substrates. Immobilized preparations have lower specific activities but retain their activities for longer than the free cells.

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References

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Author information

Authors and Affiliations

  1. Department of Chemical and Biochemical Engineering, University College London, London, WC1E 7JE, UK

    J. M. C. Duarte & M. D. Lilly

Authors
  1. J. M. C. Duarte
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  2. M. D. Lilly
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Editor information

Editors and Affiliations

  1. Corning Glass Works, Corning, New York, USA

    Howard H. Weetall

  2. The Ohio State University, Columbus, Ohio, USA

    Garfield P. Royer

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© 1980 Plenum Press, New York

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Duarte, J.M.C., Lilly, M.D. (1980). The use of Free and Immobilized Cells in the Presence of Organic Solvents: The Oxidation of Cholesterol by Nocardia Rhodochrous . In: Weetall, H.H., Royer, G.P. (eds) Enzyme Engineering. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3749-2_59

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  • DOI: https://doi.org/10.1007/978-1-4684-3749-2_59

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-3751-5

  • Online ISBN: 978-1-4684-3749-2

  • eBook Packages: Springer Book Archive

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