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Alginate Bead Entrapped Yeast Cells for Continuous Inversion of Sucrose and Molasses

  • Yu-Yen Linko
  • L. Weckstrom
  • P. Linko

Abstract

Sucrose inversion may be catalyzed either by invertase or by hydrogen ions. However, acid hydrolysis by strong cation exchange resins is generally not suitable for molasses of high ion concentration. Most work with immobilized invertase has involved isolated partially purified enzymes. Johnson and Ciegler (1) have reported the entrapment of Aspergillus oryzae spores containing active invertase in ECTEOLA-cellulose for sucrose hydrolysis in a column reactor. Toda and Shoda (2) employed Saccharomyces pastorianus cells entrapped in agar pellets for inversion in a fluidized-bed reactor. In both cases relatively low substrate concentrations were used. We have previously reported on successful immobilization of S. cerevisiae yeast cells in cellulose and cellulose diacetate beads (3). We have now developed a method to obtain a stable active biocatalyst by the entrapment of invertase active yeast cells within calcium alginate gel beads.

Keywords

Sodium Alginate Invertase Activity Cation Exchange Resin Aspergillus Oryzae Cane Molas 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    JOHNSON, J.P. & CIEGLER, A. Archiv. Biochem. Biophys. 130: 384, 1969.CrossRefGoogle Scholar
  2. 2.
    TODA, E. & SHODA, M. Biotechnol. Bioeng. 27: 481, 1975.CrossRefGoogle Scholar
  3. 3.
    LINKO, P., POUTANEN, K., WECKSTROM, L. & LINKO, Y-Y. Biochemie. (in press).Google Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Yu-Yen Linko
    • 1
  • L. Weckstrom
    • 1
  • P. Linko
    • 1
  1. 1.Department of ChemistryHelsinki University of TechnologyEspoo 15Finland

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