Continuous Isolation of Yeast-Lytic Enzymes from Citophaga
Enzymic lysis represents an alternative technique to the mechanical methods of microbial cell disruption. Yeast cells may be disrupted to obtain protein, intracellular enzymes and other material by several mechanical methods (2) which have proved to be efficient but of costly operation. On the other hand, enzymic lysis can also complement mechanical cell disruption by producing the degradation and solubilization of the cell wall. For this, a suitable enzyme has to be efficiently produced and isolated.
KeywordsAmmonium Sulphate Cell Free Supernatant Precipitate Solid Enzymic Lysis Heavy Phase
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