Advertisement

On-line Determination of Reaction Rate Versus Substrate Concentration for an Enzymatically Catalysed Reaction by Means of a Microcomputer System

  • R. Wichmann
  • C. Wandrey

Abstract

A microcomputer is used to record the change of conversion as a function of time during an enzymatically catalysed reaction. An electrical signal, which is proportional to substrate concentration is digitized and stored in constant time intervals. A sliding polynomial regression is used to smooth the concentration time curve and used to calculate the reaction rate from the first derivative of the determined polynoms (Figure 1).

Keywords

Substrate Concentration Flow Chart Electrical Signal Polynomial Regression Initial Reaction Rate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • R. Wichmann
    • 1
  • C. Wandrey
    • 1
  1. 1.Institut fuer BiotechnologieKernforschungsanlage JuelichJuelichWest Germany

Personalised recommendations