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Detoxification of Ammonia by Immobilized Urea Cycle Enzymes

  • Y. Miura
  • H. Urabe
  • K. Miyamoto
  • M. Okazaki

Abstract

The immobilization and characterization of urea cycle enzymes have been studied (1,2) because it may be possible to use them as an extracorporeal subunit of liver. The important points for an application of immobilized enzymes to an extracorporeal circulation system may be the stabilities of these enzymes, the biocompatibility, the reactivity, the homeostasis etc. In this work, we examine the stabilities of the immobilized urea cycle enzymes and the reactivity of the cycle reaction in a column reactor, and analyze the possibility for applying a hollow fiber system with the packed column reactor to the detoxification of ammonia in an extracorporeal circulation. In this study, we noted especially the two urea cycle enzymes, carbamoylphosphate synthetase (CPS) and argininosuccinate synthetase (ASS) which are known to catalyze the complicated reactions and have low specific activities and be relatively unstable in the urea cycle.

Keywords

Immobilize Enzyme Hollow Fiber Pyruvate Kinase Urea Cycle Column Reactor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    MIURA, Y., MIYAMOTO, K., RAZE, J. M. & URABE, H. J. Biomed. Mater. Res. 11: 743, 1977.CrossRefGoogle Scholar
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    MIURA, Y., MIYAMOTO, K., URABE, H., NAGATA, C., & HANE, Y. J. Biomed. Mater. Res. 11: 755, 1977.CrossRefGoogle Scholar
  3. 3.
    JONES, M. E. “Methods in Enzymology”, Vol. 5, Academic Press, 1962, p. 916.Google Scholar
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    RATNER, S. “Methods in Enzymology”, Vol. 12, Academic Press, 1970, p. 298 and 304.Google Scholar
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    ROCHOVANSKY, O. & RATNER, S. J. Biol. Chem. 236: 2254, 1961.Google Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Y. Miura
    • 1
  • H. Urabe
    • 1
  • K. Miyamoto
    • 1
  • M. Okazaki
    • 1
  1. 1.Department of Biochemical Engineering, Faculty of Pharmaceutical SciencesOsaka UniversitySuita, OsakaJapan

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