Detoxification of Ammonia by Immobilized Urea Cycle Enzymes
The immobilization and characterization of urea cycle enzymes have been studied (1,2) because it may be possible to use them as an extracorporeal subunit of liver. The important points for an application of immobilized enzymes to an extracorporeal circulation system may be the stabilities of these enzymes, the biocompatibility, the reactivity, the homeostasis etc. In this work, we examine the stabilities of the immobilized urea cycle enzymes and the reactivity of the cycle reaction in a column reactor, and analyze the possibility for applying a hollow fiber system with the packed column reactor to the detoxification of ammonia in an extracorporeal circulation. In this study, we noted especially the two urea cycle enzymes, carbamoylphosphate synthetase (CPS) and argininosuccinate synthetase (ASS) which are known to catalyze the complicated reactions and have low specific activities and be relatively unstable in the urea cycle.
KeywordsImmobilize Enzyme Hollow Fiber Pyruvate Kinase Urea Cycle Column Reactor
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