Enzyme Purification by Liquid-Liquid Extraction

  • H. Hustedt
  • K. H. Kroner
  • U. Menge
  • M.-R. Kula

Abstract

As was already demonstrated by us, aqueous two-phase systems can be used successfully for the large-scale purification of enzymes (1–3). In the examples published so far, the extraction was always one-stage process, where the combinations polyethylene glycol (PEG)/Dextran and PEG/potassium phosphate were used for constituting the two-phase systems. Using the latter which show a rather high rate of phase separation we are trying to enhance the effectivity of the phase partition method by developing continuous countercurrent procedures.

Keywords

Polyethylene Glycol Fractionation Catalase Dextran 

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References

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    KRONER, K.H., HUSTEDT, H., GRANADA, S. & KULA, M.R. Biotechn. Bioeng. 20; 1967, 1978.CrossRefGoogle Scholar
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    HUSTEDT, H., KRONER, K.H., STACH, W. & KULA, M.R. Biotechn. Bioeng. 20: 1989, 1978.Google Scholar
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    HUSTEDT, H., kroner, K.H., MENGE, U. & KULA, M.R. Prepr. 1st Eur. Congr. Biotechnol., Dechema Publisher, Frankfurt/M., W. Germany, 1978, p. 48.Google Scholar
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    MULLER, E. & KREICHELT, H.J. vt-verfahrenstechnik-13: 142, 1979.Google Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • H. Hustedt
    • 1
  • K. H. Kroner
    • 1
  • U. Menge
    • 1
  • M.-R. Kula
    • 1
  1. 1.Gesellschaft fur Biotechnologische Forschung mbHBraunschweig-StockheimFederal Republic of Germany

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