Enzyme Purification by Liquid-Liquid Extraction
As was already demonstrated by us, aqueous two-phase systems can be used successfully for the large-scale purification of enzymes (1–3). In the examples published so far, the extraction was always one-stage process, where the combinations polyethylene glycol (PEG)/Dextran and PEG/potassium phosphate were used for constituting the two-phase systems. Using the latter which show a rather high rate of phase separation we are trying to enhance the effectivity of the phase partition method by developing continuous countercurrent procedures.
KeywordsAlcian Blue Enzyme Purification Bottom Phase Countercurrent Extraction Theoretical Stage
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