Enzyme Purification by Liquid-Liquid Extraction
As was already demonstrated by us, aqueous two-phase systems can be used successfully for the large-scale purification of enzymes (1–3). In the examples published so far, the extraction was always one-stage process, where the combinations polyethylene glycol (PEG)/Dextran and PEG/potassium phosphate were used for constituting the two-phase systems. Using the latter which show a rather high rate of phase separation we are trying to enhance the effectivity of the phase partition method by developing continuous countercurrent procedures.
KeywordsPolyethylene Glycol Fractionation Catalase Dextran
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