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Enzyme-Collagen Reactor Designed from an Hemodialysis Module

  • P. R. Coulet
  • F. Paul
  • D. Dupret
  • D. C. Gautheron

Abstract

A bench scale polymembrane reactor has been developed using an artificial kidney module (RP5 model from Rhone-Poulenc, France) modified by substituting the dialysis membranes by glucohydrolases-collagen membranes prepared in our laboratory after an acyl-azide activation process (1) for the hydrolysis of a “maltodextrins” into glucose. The coupling was performed by soaking activated membranes (32.5 × 11.8 cm) in a two-enzyme solution containing 16.8 mg/ml amyloglucosidase from Aspergillus niger and 1.55 mg/ml a-amylase from Bacillus subtilis for 2 hours. The membrane activity was 150 mU/cm2 at 40°C, pH 4.8 with maltose 25 mM as standard substrate.

Keywords

Bacillus Subtilis Aspergillus Niger Maximum Conversion Membrane Activity Collagen Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    COULET, P.R., JULLIARD, J.H & GAUTHERON, D.C. Biotechnol. Bioeng. 16: 1055, 1974.CrossRefGoogle Scholar
  2. 2.
    BRILLOUET, J.M., COULET, P.R. & GAUTHERON, D.C. Biotechnol. Bioeng. 18: 1821, 1976.CrossRefGoogle Scholar
  3. 3.
    THEVENOT, D.R., STERNBERG, R., COULET, P.R., LAURENT, J. & GAUTHERON, D.C. Anal. Chem. 51: 96, 1979.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • P. R. Coulet
    • 1
  • F. Paul
    • 1
  • D. Dupret
    • 1
  • D. C. Gautheron
    • 1
  1. 1.LBTM-CNRSUniversite Claude Bernard (Lyon I)VilleurbanneFrance

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