Enzyme-Collagen Reactor Designed from an Hemodialysis Module
A bench scale polymembrane reactor has been developed using an artificial kidney module (RP5 model from Rhone-Poulenc, France) modified by substituting the dialysis membranes by glucohydrolases-collagen membranes prepared in our laboratory after an acyl-azide activation process (1) for the hydrolysis of a “maltodextrins” into glucose. The coupling was performed by soaking activated membranes (32.5 × 11.8 cm) in a two-enzyme solution containing 16.8 mg/ml amyloglucosidase from Aspergillus niger and 1.55 mg/ml a-amylase from Bacillus subtilis for 2 hours. The membrane activity was 150 mU/cm2 at 40°C, pH 4.8 with maltose 25 mM as standard substrate.
KeywordsBacillus Subtilis Aspergillus Niger Maximum Conversion Membrane Activity Collagen Membrane
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