Alternate Cellular Models for Aging Studies

  • Edward L. Schneider
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 129)


Studies such as those described by Drs. Macieira-Coelho and Mitsui lead to increased insignt into the mechanisms of the limited proliferative capacity of human diploid fibroblasts. While I suggest that these elegant studies of the in vitro passage of human cells be continued, I urge the development of alternate models for the study of cellular aging. In our laboratory, we have examined human diploid cells as a function of in vitro passage and the age of the donor of these cells. The first studies in this latter area were conducted by Drs. Hayflick, Goldstein, Martin, and their co-workers (1–3). These investigators demonstrated the diminished proliferative potential of cell cultures with the increasing age of the donor. At the Gerontology Research Center in Baltimore, we have been fortunate to be able to conduct our studies on skin fibroblast cultures derived from young and old volunteer members of the Baltimore Longitudinal Study. We have examined the total replicative ability of these skin fibroblast cultures, as well as many of the parameters analyzed by Drs. Mitsui and Macieira-Coelho as a function of in vitro passage (4).


Cellular Aging Sister Chromatid Exchange Human Diploid Fibroblast Young Donor Skin Fibroblast Culture 
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    E. L. Schneider and Y. Mitsui, The relationship between in vitro cellular aging and in vivo human age, Proc. Nat. Acad. Sci. USA 73:3584 (1976).PubMedCrossRefGoogle Scholar
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    J. R. Smith, O. M. Pereira-Smith, and E. L. Schneider, Colony size distributions as a measure of in vivo and in vitro aging, Proc. Nat. Acad. Sci. USA 75:1353 (1978).PubMedCrossRefGoogle Scholar
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    E. L. Schneider and R. E. Monticone, Aging and sister chromatid exchange II. The effect of in vitro passage level of human fetal lung fibroblasts on baseline and mutagen-induced sister chromatid exchange frequencies, Exp. Cell Res. 115:269 (1978).PubMedCrossRefGoogle Scholar
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    E. L. Schneider and B. Gilman, Sister chromatid exchanges and aging III. The effect of donor age on mutagen induced sister chromatid exchange, Hum. Genet., in press (1979).Google Scholar
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    M. D. Hollenberg and E. L. Schneider, Receptors for insulin and epidermal growth factor-urogastrone in adult human fibroblasts do not change with donor age, Mech. Ageing Dev., in press (1979).Google Scholar
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    D. Kram, E. L. Schneider, R. R. Tice, and P. Gianas, Aging and sister chromatid exchange. I. The effect of aging on mitomycin-C induced sister chromatid exchange frequencies in mouse and rat bone marrow cells in vivo, Exp. Cell Res. 114:471 (1978).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Edward L. Schneider
    • 1
  1. 1.Section on Cellular Aging and Genetics Laboratory of Cellular and Molecular Biology Gerontology Research CenterNational Institute on Aging National Institutes of HealthBaltimoreUSA

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