Mechanism of Age-Dependent Decrease in Sulfation of Chondroitin Sulfate

  • Sei-itsu Murota
  • Atsushi Honda
  • Midori Abe
  • Yo Mori
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 129)


A number of investigators have been concerned with the relationship between extracellular substances and aging (1,2). It is generally recognized that the amount of hexosamine-containing substances in tissue decreases with age, while the insoluble collagen content in the tissue increases with age (3). Recently, it has been reported that the decrease in elasticity of cartilage with age is related to the content and structure of the proteoglycan in the matrix (4).


Chondroitin Sulfate Keratan Sulfate Costal Cartilage Chondroitin Sulfate Chain Repeat Disaccharide Unit 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    H. G. Vogel, ed., “Connective Tissue and Ageing,” Excerpta Medica, Amsterdam (1973).Google Scholar
  2. 2.
    D. A. Hall, ed., “The Aging of Connective Tissue,” Academic Press, London (1976).Google Scholar
  3. 3.
    H. Boström, in: “Aging of Connective and Skeletal Tissue,” A. Engel and T. Larson, eds., Nordiska Bokhandelsforlag, Stockholm (1969).Google Scholar
  4. 4.
    S. Inerot, D. Heinegård, L. Audell, and S.-E. Olsson, Articular-cartilage proteoglycans in aging and osteoarthritis, Biochem. J. 169:143 (1978).PubMedGoogle Scholar
  5. 5.
    A. Honda, M. Abe, S.-I. Murota, and Y. Mori, The effect of aging on the synthesis of hexomsamine-containing substances from rat costal cartilage: A decrease in sulfation of chondroitin sulfate with aging, J. Biochem. 85:519 (1979).PubMedGoogle Scholar
  6. 6.
    N. Seno, K. Anno, K. Kondo, S. Nagase, and S. Saito, Improved method for electrophoretic separation and rapid quantitation of isomeric chondroitin sulfates on cellulose acetate strips, Anal. Biochem. 37:197 (1970).PubMedCrossRefGoogle Scholar
  7. 7.
    Y. H. Liau, N. I. Galicki, and M. I. Horowitz, Heterogeneity of rat rib chondroitin sulfate and susceptibility to rat gastric chondrosulfatase, Biochim. Biophys. Acta 539:315 (1978).PubMedCrossRefGoogle Scholar
  8. 8.
    M. B. Mathews and S. Glagov, Acid mucopolysaccharide patterns in aging human cartilage, J. Clin. Invest. 45:1103 (1966).PubMedCrossRefGoogle Scholar
  9. 9.
    H. Iwata, The determination and fine structures of chondroitin sulfate isomers of human cartilage and pathological tissues, J. Jap. Orthop. Assoc. 43:455 (1969).Google Scholar
  10. 10.
    H. J. Mankin and A. Z. Thrasher, The effect of age on glycosaminoglycan synthesis in rabbit articular and costal cartilages, J. Rheumatol. 4:343 (1977).PubMedGoogle Scholar
  11. 11.
    O. Habuchi, T. Yamagata, and S. Suzuki, Biosynthesis of the acetylgalactosamine 4,6-disulfate unit of squid chondroitin sulfate by transsulfation from 3′-phosphoadenosine 5′-phosphosulfate, J. Biol. Chem. 246:7357 (1971).PubMedGoogle Scholar
  12. 12.
    F. D’Ambramo and F. Lipmann, The formation of adenosine-3′-phosphate-5′-phosphosulfate in extracts of chick embryo cartilage and its conversion into chondroitin sulfate, Biochim. Biophys. Acta 25:211 (1957).CrossRefGoogle Scholar
  13. 13.
    S. Suzuki and J. L. Strominger, Enzymatic sulfation of mucopolysaccharides in hen oviduct. I. Transfer of sulfate from 3′-phosphoadenosine 5′-phosphosulfate to mucopolysaccharides, J. Biol. Chem. 235:257 (1960).PubMedGoogle Scholar
  14. 14.
    S. Suzuki and J. L. Strominger, Enzymatic sulfation of mucopolysaccharides in hen oviduct. II. Mechanism of the reaction studied with oligosaccharides and monosaccharides as acceptors, J. Biol. Chem. 235:267 (1960).PubMedGoogle Scholar
  15. 15.
    S. Suzuki and J. L. Strominger, Enzymatic sulfation of mucopolysaccharides in hen oviduct. III. Mechanism of sulfation of chondroitin and chondroitin sulfate A, J. Biol. Chem. 235:274 (1960).PubMedGoogle Scholar
  16. 16.
    H. C. Robinson, The sulfation of chondroitin sulfate in embryonic chicken cartilage, Biochem. J. 113:543 (1969).PubMedGoogle Scholar
  17. 17.
    S. DeLuca, M. E. Richmond, and J. E. Silbert, Biosynthesis of chondroitin sulfate: Sulfation of the polysaccharide chain, Biochemistry 12:3911 (1973).PubMedCrossRefGoogle Scholar
  18. 18.
    G. W. Hart, Glycosaminoglycan sulfotransferases of the developing chick cornea, J. Biol Chem. 253:347 (1978).PubMedGoogle Scholar
  19. 19.
    H. Saito, T. Yamagata, and S. Suzuki, Enzymatic methods for the determination of small quantitites of isomeric chondroitin sulfates, J. Biol. Chem. 243:1536 (1968).PubMedGoogle Scholar
  20. 20.
    Z. Simunek and H. Muir, Changes in the protein-polysaccharides of pig articular cartilage during prenatal life, development and old age, Biochem. J. 126:515 (1972).PubMedGoogle Scholar
  21. 21.
    D. Heinegård and V. C. Hascall, Aggregation of cartilage proteoglvcans. III. Characteristics of the proteins isolated from trypsin digests of aggregrates, J. Biol. Chem. 249:4250 (1974).PubMedGoogle Scholar
  22. 22.
    R. Silberberg and P. Lesker, Enzyme activity in aging articular cartilage, Experientia 27:133 (1971).PubMedCrossRefGoogle Scholar
  23. 23.
    A. Wasteson, U. Lindahl, and A. Hallén, Mode of degradation of the chondroitin sulfate proteoglycan in rat costal cartilage, Biochem. J. 130:729 (1972).PubMedGoogle Scholar
  24. 24.
    J. D. Salvo and M. Schubert, Specific interaction of some cartilage protein-polysaccharides with freshly precipitating calcium phosphate, J. Biol. Chem. 242:705 (1967).PubMedGoogle Scholar
  25. 25.
    R. D. Campo, C. D. Tourtellotte, and R. J. Bielen, The protein-polysaccharides of articular, epiphyseal plate and costal cartilages, Biochim. Biophys. Acta 177:501 (1969).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Sei-itsu Murota
    • 1
  • Atsushi Honda
    • 1
    • 2
  • Midori Abe
    • 1
  • Yo Mori
    • 2
  1. 1.Department of PharmacologyTokyo Metropolitan Institute of GerontologyItabashi-ku, Tokyo 173Japan
  2. 2.Department of BiochemistryTokyo College of PharmacyHachioji-shi, Tokyo 192-03Japan

Personalised recommendations