Multinucleation and Polyploidization of Aging Human Cells in Culture

  • Toshiharu Matsumura
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 129)


Multinucleated cells and polyploid cells appear widely in liver, salivary glands, and other mammalian organs. In liver, the multi-nucleation and polyploidization of hepatocytes are the functions of development, nutrition, regeneration and aging (1,2,3,4). A schematic genealogy of cells has been proposed for the development of multinucleation and polyploidization in rodent liver (2).


Multinucleated Cell Binucleated Cell Polyploid Cell Population Doubling Level Clonogenic Cell 
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  1. 1.
    H. Tauchi and T. Sato, Some micromeasuring studies of hepatic cells in senility, J. Gerontol. 17:254 (1962).PubMedGoogle Scholar
  2. 2.
    C. Nadal and F. Zajdela, Polyploidie somatique dans le foie de rat. I. Le role des cellules binucleees dans la genese des cellules polyploides, Exp. Cell Res. 42:99 (1966).PubMedCrossRefGoogle Scholar
  3. 3.
    D. N. Wheatley, Binucleation in mammalian liver: Studies on the control of cytokinesis in vivo, Exp. Cell Res. 74:455 (1972).PubMedCrossRefGoogle Scholar
  4. 4.
    W. Ya. Brodsky and I. V. Uryvaeva, Cell polyploidy: Its relation to tissue growth and function, Int. Rev. Cytol. 50:275 (1977).PubMedCrossRefGoogle Scholar
  5. 5.
    L. Hayflick and P. S. Moorhead, The serial cultivation of human diploid cell strains, Exp. Cell Res. 25:585 (1961).CrossRefGoogle Scholar
  6. 6.
    A. Maciera-Coelho, J. Ponten, and L. Philipson, Inhibition of the division cycle in confluent cultures of human fibroblasts in vitro, Exp. Cell Res. 43:20 (1966).CrossRefGoogle Scholar
  7. 7.
    E. Saksela and P. S. Moorhead, Aneuploidy in the degenerative phase of serial cultivation of human cell strains, Proc. Natl, Acad. Sci. 50:390 (1963).CrossRefGoogle Scholar
  8. 8.
    M. C. Yoshida and S. Makino, A chromosome study of non-treated and an irradiated human in vitro cell line, Japan J. Human Gen. 5:39 (1963).Google Scholar
  9. 9.
    Z. K. Kadanka, J. D. Sparkes, and H. G. Macmorine, A study of the cytogenetics of the human cell strain WI-38, In Vitro 8:353 (1973).Google Scholar
  10. 10.
    R. C. Miller, W. W. Nichols, J. Pottash, and M. M. Aronson, In vitro aging: Cytogenetic comparison of diploid human fibroblasts and epitheliod cell lines, Exp. Cell Res. 110:63 (1977).PubMedCrossRefGoogle Scholar
  11. 11.
    T. Matsumura, Z. Zerrudo, and L. Hayflick, Senescent human diploid cells in culture: Survival, DNA synthesis and morphology, J. Gerontol., (1977) (in press).Google Scholar
  12. 12.
    T. Matsumura and S. Miyashita, Characteristics of human liver epithelial-like cells proliferating in culture, Proc. Japan Cancer Assoc, 37th Annual Meeting 120 (1978).Google Scholar
  13. 13.
    J. G. Rheinwald and H. Green, Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes, Nature 265:421 (1977).PubMedCrossRefGoogle Scholar
  14. 14.
    H. Imai, Human embryonic liver cell in culture. A preliminary report, Okayama Igakkai Zasshi 89:237 (1977).Google Scholar
  15. 15.
    R. A. Richman, T. H. Claus, S. Pilkis, and D. L. Friedman, Hormonal stimulation of DNA synthesis in primary cultures of adult rat hepatocytes, Proc. Natl. Acad. Sci. USA 73:3579 (1976).CrossRefGoogle Scholar
  16. 16.
    A. Ichihara, Relation of the characteristics of liver cells during culture, differentiation, and carcinogenesis, in: “Control Mechanisms in Cancer,” W. E. Criss, T. Ono, and J. R. Sabine, eds., Raven Press, New York (1976).Google Scholar
  17. 17.
    J. Bausher and W. I. Schaeffer, A diploid rat liver cell culture. I. Characterization and sensitivity to alfatoxin B1, In Vitro 9:286 (1974).Google Scholar
  18. 18.
    H. Masuji, H. Nakabayashi, and J. Sato, Long-term cultivation of two diploid epithelial cell lines derived from normal rat liver cells, Acta Med. Okayama 28:281 (1974).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Toshiharu Matsumura
    • 1
  1. 1.Department of Cancer Cell Research Institute of Medical ScienceUniversity of TokyoMinato-ku Tokyo 108Japan

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