Change of Responsiveness to Growth Stimulation of Normal Cells during Aging

  • Tadao Ohno
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 129)


The sole determinant of life span of normal diploid cells in vitro has been considered to be the intrinsic doubling number of the cells (1,2,3). Recent investigations, however, suggest that extracellular factors can affect the proliferative potential of the cells. For example, hydrocortisone (4) and epidermal growth factor (5) elongate the life span of fibroblasts and keratinocytes, respectively. In vivo experiments also suggest that the milieu in old animals reduces the growth potential of hematopoietic stem cells (6). However, quantitative relationships between cellular growth potential and factors which are supposed, or have been known, to influence cellular growth potential are obscure. This report describes the relationship between the amount of growth stimulating factors and cellular growth potential in the course of aging in vitro, and discusses a possible growth property of divergent cells in a cell population.


Constant Growth Rate Human Lung Fibroblast Growth Stimulate Factor Population Doubling Level Dialyze Fetal Bovine Serum 
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Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Tadao Ohno
    • 1
  1. 1.National Institute of Radiological SciencesAnagawa, Chiba-shi 260Japan

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