Kinetics of the Proliferation of Human Fibroblasts during Serial Subcultivation inVitro
The studies of the kinetics of cell proliferation in vitro have been plagued by the tendency to establish clear-cut limits to the behavior of cells in regard to the division cycle. Investigators in the field have predominantly thought in terms of compartments instead of gradients, measured instants instead of variations during long periods of time, and expressed the results in terms of means instead of distributions.
KeywordsHuman Fibroblast Early Passage Saturation Density Late Passage Population Doubling Level
- 6.A. Macieira-Coelho, Cell cycle analysis in mammalian cells, in: “Tissue Culture: Methods and Applications,” P. F. Kruse, Jr. and M. K. Patterson, Jr., eds., Academic Press, New York (1973).Google Scholar